1701. Poročilo o preskusu št.: LVG 2024-038 : vzorec št. 2024/00049Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, izvedensko mnenje, arbitražna odločba Ključne besede: varstvo gozdov, morfološke analize, Quercus ilex, sadike, šibki patogeni, molekularne analize, javna gozdarska služba
Objavljeno v DiRROS: 23.05.2024; Ogledov: 336; Prenosov: 182
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1703. Poročilo o preskusu št.: LVG 2024-022 : vzorec št. 2024/00043/THTine Hauptman, Špela Hočevar, Barbara Piškur, 2024, izvedensko mnenje, arbitražna odločba Ključne besede: varstvo gozdov, morfološke analize, Quercus petraea, Fusarium sp., Tolypocladium sp., molekularne analize, javna gozdarska služba
Objavljeno v DiRROS: 23.05.2024; Ogledov: 294; Prenosov: 199
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1704. Poročilo o preskusu št.: LVG 2024-021 : vzorec št. 2024/00006/G4Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, izvedensko mnenje, arbitražna odločba Ključne besede: varstvo gozdov, morfološke analize, Carpinus betulus, Phaeoacremonium fraxinopennsylvanicum, Anisandrus dispar, javna gozdarska služba
Objavljeno v DiRROS: 23.05.2024; Ogledov: 313; Prenosov: 231
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1705. Genotoxicity and heating performance of VxFe3-xO4 nanoparticles in health applicationsBeatriz Sanz-Sagué, Amaia Sáenz-Hernández, Bojana Žegura, Alja Štern, Katja Kološa, Iza Rozman, 2024, izvirni znanstveni članek Povzetek: The applications of magnetic nanoparticles (MNPs) as biocatalysts in different biomedical areas have been evolved very recently. One of the main challenges in this field is to design affective MNPs surfaces with catalytically active atomic centres, while producing minimal toxicological side effects on the hosting cell or tissues. MNPs of vanadium spinel ferrite (VFe2O4) are a promising material for mimicking the action of natural enzymes in degrading harmful substrates due to the presence of active V5+ centres. However, the toxicity of this material has not been yet studied in detail enough to grant biomedical safety. In this work, we have extensively measured the structural, compositional, and magnetic properties of a series of VxFe3-xO4 spinel ferrite MNPs to assess the surface composition and oxidation state of V atoms, and also performed systematic and extensive in vitro cytotoxicity and genotoxicity testing required to assess their safety in potential clinical applications. We could establish the presence of V5+ at the particle surface even in water-based colloidal samples at pH 7, as well as different amounts of V2+ and V3+ substitution at the A and B sites of the spinel structure. All samples showed large heating efficiency with Specific Loss Power values up to 400 W/g (H0 = 30 kA/m; f = 700 kHz). Samples analysed for safety in human hepatocellular carcinoma (HepG2) cell line with up to 24h of exposure showed that these MNPs did not induce major genomic abnormalities such as micronuclei, nuclear buds, or nucleoplasmic bridges (MNIs, NBUDs, and NPBs), nor did they cause DNA double-strand breaks (DSBs) or aneugenic effects—types of damage considered most harmful to cellular genetic material. The present study is an essential step towards the use of these type of nanomaterials in any biomedical or clinical application.
Ključne besede: magnetic nanoparticles, vanadium ferrite, cytotoxicity, genotoxicity, specific power absorption, cell viability
Objavljeno v DiRROS: 23.05.2024; Ogledov: 420; Prenosov: 386
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1706. Adsorption efficiency of cadmium (II) by different alkali-activated materialsNataša Mladenović Nikolić, Ljiljana Kljajević, Snežana B. Nenadović, Jelena Potočnik, Sanja Knežević, Sabina Dolenec, Katarina Trivunac, 2024, izvirni znanstveni članek Povzetek: The objective of this study was to demonstrate the potential utilization of fly ash (FA), wood ash (WA), and metakaolin (MK) in developing new alkali-activated materials (AAMs) for the removal of cadmium ions from waste water. The synthesis of AAMs involved the dissolution of solid precursors, FA, WA, and MK, by a liquid activator (Na2SiO3 and NaOH). In concentrated solutions of the activator, the formation of an aluminosilicate gel structure occurred. DRIFT spectroscopy of the AAMs indicated main vibration bands between 1036 cm−1 and 996 cm−1, corresponding to Si-O-Si/Si-O-Al bands. Shifting vibration bands were seen at 1028 cm−1 to 1021 cm−1, indicating that the Si-O-Si/Si-O-Al bond is elongating, and the bond angle is decreasing. Based on the X-ray diffraction results, alkali-activated samples consist of an amorphous phase and residual mineral phases. The characteristic “hump” of an amorphous phase in the range from 20 to 40◦ 2θ was observed in FA and in all AWAFA samples. By the XRD patterns of the AAMs obtained by the activation of a solid three-component system, a new crystalline phase, gehlenite, was identified. The efficiency of AAMs in removing cadmium ions from aqueous solutions was tested under various conditions. The highest values of adsorption capacity, 64.76 mg/g (AWAFA6), 67.02 mg/g (AWAFAMK6), and 72.84 mg/gmg/g (AWAMK6), were obtained for materials activated with a 6 M NaOH solution in the alkali activator. The Langmuir adsorption isotherm and pseudo-second kinetic order provided the best fit for all investigated AAMs.
Ključne besede: fly ash, metakaolin, wood ash, adsorption kinetics, cadmium (II)
Objavljeno v DiRROS: 23.05.2024; Ogledov: 398; Prenosov: 322
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1707. Susceptibility of X17CrNi16-2 martensitic stainless steel to hydrogen embrittlement after conventional and deep cryogenic heat treatmentMirjam Bajt Leban, Bojan Zajec, Bojan Podgornik, Črtomir Donik, Patricia Jovičević Klug, Miha Hren, Andraž Legat, 2024, izvirni znanstveni članek Povzetek: A low carbon/ high chromium martensitic stainless steel, X17CrNi16-2, was heat treated using two different hardening and tempering regimes (1050 ◦ C/480 ◦ C or 980 ◦C/600 ◦C) — promoting either a high strength or high toughness state, respectively and further combined with deep cryogenic treatment (DCT) at 196 ◦C for 24 h. Over recent years DCT has been recognized as a promising technique to improve the properties of steel, predominantly with respect to its tensile strength, toughness and wear resistance. The influence of DCT on the hydrogen embrittlement resistance of martensitic stainless steel has not yet, however, been reported.
A slow strain rate tensile test (SSRT) with simultaneous cathodic hydrogen charging was selected as the method to assess potential susceptibility to hydrogen embrittlement (HE). Relatively low-intensity hydrogen charging, utilizing a constant current density of 0.1 mA/cm2, in a non-corrosive, slightly alkaline buffer solution, led to a clear reduction in the ultimate tensile stress. This reduction, and therefore the HE susceptibility, was more pronounced in the steel with a higher strength (i.e. that subject to the 1050 ◦C/ 480 ◦C heat treatment condition). Furthermore, DCT did not appear to have any impact on the steel’s mechanical properties in the presence of hydrogen. Fractographic analysis showed clear evidence of HE in the hydrogen-charged specimens.
This paper presents results of the SSRT tests and further fractography results, and discusses the impact of conventional and deep cryogenic treatment on HE susceptibility.
Ključne besede: martensitic stainless steel, deep cryogenic treatment, hydrogen embrittlement, slow strain rate tensile test
Objavljeno v DiRROS: 23.05.2024; Ogledov: 446; Prenosov: 385
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1708. Compensational role between cathepsinsUrša Pečar Fonović, Janko Kos, Ana Mitrović, 2024, pregledni znanstveni članek Povzetek: Cathepsins, a family of lysosomal peptidases, play a crucial role in maintaining cellular homeostasis by regulating protein turnover and degradation as well as many specific regulatory actions that are important for proper cell function and human health. Alterations in the activity and expression of cathepsins have been observed in many diseases such as cancer, inflammation, neurodegenerative disorders, bone remodelling-related conditions and others. These changes are not exclusively harmful, but rather appear to be a compensatory response on the lack of one cathepsin in order to maintain tissue integrity. The upregulation of specific cathepsins in response to the inhibition or dysfunction of other cathepsins suggests a fine-tuned system of proteolytic balance and understanding the compensatory role of cathepsins may improve therapeutic potential of cathepsin's inhibitors. Selectively targeting one cathepsin or modulating their activity could offer new treatment strategies for a number of diseases. This review emphasises the need for comprehensive research into cathepsin biology in the context of disease. The identification of the specific cathepsins involved in compensatory responses, the elucidation of the underlying molecular mechanisms and the development of targeted interventions could lead to innovative therapeutic approaches.
Ključne besede: lysosomal peptidases, compensation, bone resorption
Objavljeno v DiRROS: 23.05.2024; Ogledov: 363; Prenosov: 253
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