1. Production of volatile moth sex pheromones in transgenic Nicotiana benthamiana plantsRubèn Mateos Fernández, Elena Moreno Gimenéz, Silvia Gianoglio, Alfredo Quijano-Rubio, Jose Gavaldá-García, Lucía Estellés, Alba Rubert, José Luis Rambla, Marta Vazquez-Vilar, Estefanía Huet, Asunción Fernández-del-Carmen, Ana Espinosa-Ruiz, Mojca Juteršek, Sandra Vacas, Ismael Navarro Fuertes, Vicente Navarro-Llopis, Jaime Primo, Diego Orzaez, 2021, izvirni znanstveni članek Povzetek: Plant-based bioproduction of insect sex pheromones has been proposed as an innovative strategy to increase the sustainability of pest control in agriculture. Here, we describe the engineering of transgenic plants producing (Z)-11-hexadecenol (Z11-16OH) and (Z)-11-hexadecenyl acetate (Z11-16OAc), two main volatile components in many Lepidoptera sex pheromone blends. We assembled multigene DNA constructs encoding the pheromone biosynthetic pathway and stably transformed them into Nicotiana benthamiana plants. The constructs contained the Amyelois transitella AtrΔ11 desaturase gene, the Helicoverpa armigera fatty acyl reductase HarFAR gene, and the Euonymus alatus diacylglycerol acetyltransferase EaDAct gene in different configurations. All the pheromone-producing plants showed dwarf phenotypes, the severity of which correlated with pheromone levels. All but one of the recovered lines produced high levels of Z11-16OH, but very low levels of Z11-16OAc, probably as a result of recurrent truncations at the level of the EaDAct gene. Only one plant line (SxPv1.2) was recovered that harboured an intact pheromone pathway and which produced moderate levels of Z11-16OAc (11.8 μg g-1 FW) and high levels of Z11-16OH (111.4 μg g-1). Z11-16OAc production was accompanied in SxPv1.2 by a partial recovery of the dwarf phenotype. SxPv1.2 was used to estimate the rates of volatile pheromone release, which resulted in 8.48 ng g-1 FW per day for Z11-16OH and 9.44 ng g-1 FW per day for Z11-16OAc. Our results suggest that pheromone release acts as a limiting factor in pheromone biodispenser strategies and establish a roadmap for biotechnological improvements.
Objavljeno v DiRROS: 19.07.2024; Ogledov: 34; Prenosov: 26
 Celotno besedilo (1,99 MB)
Gradivo ima več datotek! Več... |
2. Transcriptional deregulation of stress-growth balance in Nicotiana benthamiana biofactories producing insect sex pheromonesMojca Juteršek, Marko Petek, Živa Ramšak, Elena Moreno Gimenéz, Silvia Gianoglio, Rubèn Mateos Fernández, Diego Orzaez, Kristina Gruden, Špela Baebler, 2022, izvirni znanstveni članek Povzetek: Plant biofactories are a promising platform for sustainable production of high-value compounds, among which are insect sex pheromones, a green alternative to conventional insecticides in agriculture. Recently, we have constructed transgenic Nicotiana benthamiana plants (“Sexy Plants”, SxP) that successfully produce a blend of moth (Lepidoptera) sex pheromone compounds (Z)-11-hexadecen-1-ol and (Z)-11-hexadecenyl acetate. However, efficient biosynthesis of sex pheromones resulted in growth and developmental penalty, diminishing the potential for commercial use of SxP in biomanufacturing. To gain insight into the underlying molecular responses, we analysed the whole-genome transcriptome and evaluated it in relation to growth and pheromone production in low- and high-producing transgenic plants of v1.0 and v1.2 SxP lines. In our study, high-producing SxPv1.2 plants accumulated the highest amounts of pheromones but still maintained better growth compared to v1.0 high producers. For an in-depth biological interpretation of the transcriptomic data, we have prepared a comprehensive functional N. benthamiana genome annotation as well as gene translations to Arabidopsis thaliana, enabling functional information transfer by using Arabidopsis knowledge networks. Differential gene expression analysis, contrasting pheromone producers to wild-type plants, revealed that while only a few genes were differentially regulated in low-producing plants, high-producing plants exhibited vast transcriptional reprogramming. They showed signs of stress-like response, manifested as downregulation of photosynthesis-related genes and significant differences in expression of hormonal signalling and secondary metabolism-related genes, the latter presumably leading to previously reported volatilome changes. Further network analyses confirmed stress-like response with activation of jasmonic acid and downregulation of gibberellic acid signalling, illuminating the possibility that the observed growth penalty was not solely a consequence of a higher metabolic burden imposed upon constitutive expression of a heterologous biosynthetic pathway, but rather the result of signalling pathway perturbation. Our work presents an example of comprehensive transcriptomic analyses of disadvantageous stress signalling in N. benthamiana biofactory that could be applied to other bioproduction systems.
Ključne besede: transcriptomics, plant biotechnology, jasmonic acid, growthstress tradeoffs, network analysis, growth penalty, insect sex pheromone
Objavljeno v DiRROS: 17.07.2024; Ogledov: 48; Prenosov: 14
Celotno besedilo (7,68 MB)
Gradivo ima več datotek! Več... |
3. Analysis of the geological control on the spatial distribution of potentially toxic concentrations of As and F- in groundwater on a Pan-European scaleElena Giménez-Forcada, Juan Antonio Luque-Espinar, María Teresa López-Bahut, Juan Grima-Olmedo, Jorge Jiménez-Sánchez, Carlos Ontiveros-Beltranena, José Angel Díaz-Muñoz, Daniel Elster, Ferid Skopljak, Denitza D. Voutchkova, Birgitte Hansen, Klaus Hinsby, Jörg Schullehner, Eline Malcuit, Laurence Gourcy, Teodóra Szőcs, Nóra Gál, Daði Þorbjörnsson, Katie Tedd, Dāvis Borozdins, Henry Debattista, Nina Rman, 2022, izvirni znanstveni članek Povzetek: The distribution of the high concentrations of arsenic (As) and fluoride (F-) in groundwater on a Pan-European scale could be explained by the geological European context (lithology and structural faults). To test this hypothesis, seventeen countries and eighteen geological survey organizations (GSOs) have participated in the dataset. The methodology has used the HydroGeoToxicity (HGT) and the Baseline Concentration (BLC) index. The results prove that most of the waters considered in this study are in good conditions for drinking water consumption, in terms of As and/or F- content. A low proportion of the analysed samples present HGT≥ 1 levels (4% and 7% for As and F-, respectively). The spatial distribution of the highest As and/or F- concentrations (via BLC values) has been analysed using GIS tools. The highest values are identified associated with fissured hard rock outcrops (crystalline rocks) or Cenozoic sedimentary zones, where basement fractures seems to have an obvious control on the distribution of maximum concentrations of these elements in groundwaters.
Ključne besede: trace elements, arsenic fluoride, groundwater, geo-hydrochemistry, spatial analysis
Objavljeno v DiRROS: 30.01.2023; Ogledov: 1139; Prenosov: 267
Celotno besedilo (16,39 MB)
Gradivo ima več datotek! Več... |
4. Terminologies and characteristics of natural mineral and thermal waters in selected European countriesDaniel Elster, Teodóra Szőcs, Nóra Gál, Birgitte Hansen, Denitza D. Voutchkova, Jörg Schullehner, Julie Lions, Lucio Martarelli, Elena Giménez-Forcada, José Angel Díaz-Muñoz, Eline Malcuit, Gerhard Schubert, Gerhard Hobiger, Nina Rman, 2022, pregledni znanstveni članek Povzetek: This study discusses 1) the national legislative frameworks, terminologies, and criteria for the recognition of natural mineral waters and thermal waters in selected European countries (Austria, Bosnia and Herzegovina, Denmark, France, Hungary, Iceland, Italy, Lithuania, Poland, Portugal, Romania, Serbia, Slovenia, and Spain), and 2) it provides a first extensive multi-national overview of hydrogeological and hydrogeochemical characteristics of numerous water sources from those regions.
Ključne besede: hydrogeology, regulatory framework, hydrogeochemical composition, natural mineral water, thermal water, Europe
Objavljeno v DiRROS: 26.07.2022; Ogledov: 849; Prenosov: 289
Celotno besedilo (10,31 MB) |
