1. Potato (Solanum tuberosum) - a new host for the root-knot nematode Meloidogyne inornataBarbara Gerič Stare, Nik Susič, Maja Starovič, Saša Širca, 2022, drugi znanstveni članki Ključne besede: pathogenicity, plant-parasitic nematodes, nematodes, root-knot nematodes, potato Objavljeno v DiRROS: 27.07.2023; Ogledov: 528; Prenosov: 228
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2. Analysis of putative apoplastic effectors from the nematode, Globodera rostochiensis, and identification of an expansin-like protein that can induce and suppress host defensesBarbara Gerič Stare, Shawkat Ali, Maxime Magne, Shiyan Chen, Olivier Côte, Natasa Obradovic, Lubna Jamshaid, Xiaohong Wang, Guy Bélair, Peter Moffett, 2015, izvirni znanstveni članek Ključne besede: nematode, expansin-like protein, expansin, potato cyst nematode, potato pest, parasitic nematodes, plant celular function Objavljeno v DiRROS: 06.09.2022; Ogledov: 677; Prenosov: 250
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3. Uporaba naprednih tehnologij za celostno obvladovanje ogorčic koreninskih šišk (Meloidogyne spp., Nematoda: Meloidogynidae)Nik Susič, 2020, doktorska disertacija Povzetek: Tropske vrste ogorčic koreninskih šišk (RKN) so izredno polifagni škodljivci v kmetijstvu, kjer povzročajo velike izgube pridelka. Nekatere vrste, kot so Meloidogyne ethiopica, M. inornata in M. luci (MEG), je zaradi visoke sorodnosti težavno razlikovati. Sekvencirali smo 7 izolatov ogorčic iz skupine MEG ter po zlaganju zaporedij genomov določili filogenetsko umestitev v rod. Zloženi genom ogorčice M. luci SI-Smartno V13 je s 327 kontigi, N50 dolžini kontigov 1,7 Mb in končni dolžini zloženega zaporedja 209,2 Mb, trenutno najpopolnejši javno dostopen genom RKN. Filogenetska analiza je pokazala na umestitev vseh izolatov iz skupine MEG v enoten monofiletski klad, vrsta M. luci se je bistveno razlikovala od vrst M. ethiopica in M. inornata. Preizkušali smo uporabnost hiperspektralnega slikanja za zgodnje razločevanje biotskega stresa (napad RKN) od abiotskega stresa (suša) pri rastlinah paradižnika. Z analizo hiperspektralnih posnetkov v območju spektra 400-2500 nm je bilo mogoče razlikovati med dobro zalitimi ter sušnimi rastlinami s 100 % natančnostjo, z 90-100 % natančnostjo pa je bilo mogoče razlikovati med zdravimi in napadenimi rastlinami. Ovrednotili smo nematicidno aktivnost in analizirali genoma bakterij Bacillus firmus I-1582 in Bacillus sp. ZZV12-4809, v katerih smo našli številne potencialne dejavnike virulence. V lončnih poskusih in na mikroparcelah je sev I-1582 zmanjšal število ogorčic M. luci za 51-53 % v primerjavi s kontrolo. Bakterije I-1582 so delovale nematicidno ter spodbudile rast rastlin, kar smo pokazali z meritvami morfologije rastlin, relativne vsebnosti klorofila, vsebnostjo elementov in analizo hiperspektralnih posnetkov. Z analizo hiperspektralnih posnetkov z nadzorovano klasifikacijo smo uspešno razlikovali med rastlinami tretiranimi z B. firmus in rastlinami brez bakterij – v lončnem poskusu smo dosegli 97,4 % uspešnost, na mikroparcelah pa 96,3 %. Ključne besede: ogorčice koreninskih šišk, filogenomika, hiperspektralno slikanje, biotično varstvo rastlin, Meloidogyne spp., Bacillus firmus, doktorske disertacije Objavljeno v DiRROS: 06.09.2022; Ogledov: 927; Prenosov: 283
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4. Microarray-based uncovering of reference genes for quantitative real-time PCR in potato tuber infected with PVYBarbara Gerič Stare, Aleš Sedlar, Vladimir Meglič, 2021, izvirni znanstveni članek Ključne besede: potato, potato tuber, tuber infection, potato virus Y, real-time PCR, PCR, polymerase chain reaction, reference genes, stored ppotato tubers, pathogens Objavljeno v DiRROS: 31.05.2021; Ogledov: 1630; Prenosov: 366
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6. Record of a new location for tropical root knot nematode Meloidogyne luci in SloveniaBarbara Gerič Stare, Polona Strajnar, Saša Širca, Nik Susič, Gregor Urek, izvirni znanstveni članek Povzetek: The plant parasitic nematode Meloidogyne ethiopica Whitehead has been reported from several European countries, including Slovenia, where it was first found in 2003. However, the description in 2014 of a new sister species, Meloidogyne luci Carneiro et al., 2014, called for the reclassification of all European and Turkish populations of M. ethiopica reported up to this date as M. luci. Both species M. ethiopica and M. luci have a very wide host range of
monocotyledons and dicotyledons. They are pests of important agricultural crops such as maize, potatoes and variety of vegetables, and represent a serious threat for agricultural production in greenhouses as well as in the open fields in many EPPO countries. This paper reports a new location of the tropical root knot nematode species M. luci found in Slovenia in 2015. Ključne besede: parasitic nematode, root knot nematode, classification, populations Objavljeno v DiRROS: 20.07.2018; Ogledov: 2983; Prenosov: 1275
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7. Discrimination between abiotic and biotic drought stress in tomatoes using hyperspectral imagingNik Susič, Uroš Žibrat, Saša Širca, Polona Strajnar, Jaka Razinger, Matej Knapič, Andrej Vončina, Gregor Urek, Barbara Gerič Stare, izvirni znanstveni članek Povzetek: Crop plants are subjected to various biotic and abiotic stresses. Both root-knot nematodes (biotic stress) and water deficiency (abiotic stress) lead to similar drought symptoms in the plant canopy. In this work, hyperspectral imaging was used for early detection of nematode infestation and water deficiency (drought) stress in tomato plants. Hyperspectral data in the range from 400 to 2500 nm of plants subjected to different watering regimes and nematode infestation levels were analysed by partial least squares – discriminant analysis (PLS-DA) and partial least squares – support vector machine (PLS-SVM) classification. PLS-SVM classification achieved up to 100% accuracy differentiating between well-watered and water-deficient plants, and between 90 and 100% when identifying nematode-infested plants. Grouping the data according to the time of imaging increased the accuracy of classification. Shortwave infrared spectral regions associated with the OH and CH stretches were most relevant for the identification of nematode infested plants and severity of infestation. This study demonstrates the capability of hyperspectral imaging to identify and discriminate between biotic and abiotic plant stresses. Ključne besede: nematode, Hyperspectral imaging, Drought stress, Root-knot nematode, Tomato Objavljeno v DiRROS: 20.07.2018; Ogledov: 4108; Prenosov: 2558
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