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Povzetek: Small RNAs (sRNAs) are short noncoding RNAs involved in the regulation of a wide range of biological processes in plants. Advances in high-throughput sequencing and development of new computational tools had facilitated the discovery of different classes of sRNAs, their quantification, and elucidation of their functional role in gene expression regulation by target transcript predictions. The workflow presented here allows identification of different sRNA species: known and novel potato miRNAs, and their sequence variants (isomiRs), as well as identification of phased small interfering RNAs (phasiRNAs). Moreover, it includes steps for differential expression analysis to search for regulated sRNAs across different tested biological conditions. In addition, it describes two different methods for predicting sRNA targets, in silico prediction, and degradome sequencing data analysis. All steps of the workflow are written in a clear and user-friendly way; thus they can be followed also by the users with minimal bioinformatics knowledge. We also included several in-house scripts together with valuable notes to facilitate data (pre)processing steps and to reduce the analysis time.
Objavljeno v DiRROS: 06.08.2025; Ogledov: 421; Prenosov: 205
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Povzetek: The paper examines Serbian press coverage of the resurgence of Eastern Ortho-doxy in Serbia during the 1980s. It is based on an analysis of the most influential daily, weekly, and monthly newspapers and magazines printed in Serbia between 1980 and 1990. The research aims to reconstruct perceptions of the revival of re-ligiosity in Serbia during this period and to identify its root causes. The research showed a close causal connection between the change in public perception of religion, that is, the revival of religiosity in Serbia, and the changed political en-vironment in former Yugoslavia in the 1980s. The research suggests that the esca-lation of tensions in Kosovo triggered the ethnic mobilization of Serbian society and consequently strengthened the influence of the Serbian Orthodox Church in Serbia in the late 1980s
Ključne besede: Serbia, religiosity, 1980s, Yugoslavia, Eastern Ortodoxy
Objavljeno v DiRROS: 06.08.2025; Ogledov: 262; Prenosov: 191
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Povzetek: This article presents the concept and dynamics of secularization policies and the atheization process in Montenegrin society following World War II. The pro-cess of secularization began with the Communist party’s rise to power and had its most significant manifestations until the mid-1950s. In this research, in addition to the specific secularization policies of the communist authorities, an analysis of the relationship of the state, that is, the communist authorities, towards the religious communities in Montenegro is given. Montenegro was the Yugoslav republic in which the process of atheization and secularization took place most intensively in relation to the federal level. The research presented in the article also includes an analysis of the results of the secularization of the three largest religious commu-nities in Montenegro.
Ključne besede: secularization, communism, Montenegro, religious communities, atheism
Objavljeno v DiRROS: 06.08.2025; Ogledov: 302; Prenosov: 168
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Povzetek: Background Shear flow deformability cytometry is an emerging microfluidic technique that has undergone significant advances in the last few years and offers considerable potential for clinical diagnostics and disease monitoring. By simultaneously measuring mechanical and morphological parameters of single cells, it offers a comprehensive extension of traditional cell analysis, delivering unique insight into cell deformability, which is gaining recognition as a novel biomarker for health and disease. Due to its operating principle, the method is particularly suitable for the clinical analysis of blood samples. Results This review focuses on the recent developments in shear flow deformability cytometry, which is a widely adopted variant of deformability cytometry. It has a strong potential for applications in clinical practice due to its robust and simple operation, demonstrated applications with whole blood samples, as well as its high throughput, which can reach approximately 1000 cells per second. We begin by discussing some basic factors that influence the mechanical properties of cells and give an overview of deformability cytometry and its operational principles for samples from blood, cultured cells and tissues. Next, we review recent clinically relevant applications in analysis of blood and cancer cells. Finally, we address key challenges to clinical adoption, such as regulatory approval, scalable manufacturing, and workflow integration, emphasizing the need for further validation studies to facilitate clinical implementation. Significance This article uniquely emphasizes the clinical relevance of microfluidic shear flow deformability cytometry, by giving an overview of mechanical and morphological biomarkers studied in clinically significant samples. In addition, it addresses critical barriers to clinical translation. By identifying these obstacles, this article aims to demonstrate the potential of deformability cytometry to bridge the gap between the research and the routine medical practice.
Ključne besede: deformability cytometry, microfluidics, cell mechanics, mechanical biomarkers, blood analysis, disease, single-cell, clinical method
Objavljeno v DiRROS: 06.08.2025; Ogledov: 342; Prenosov: 215
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Povzetek: particularly in comparison with the neighbouring republic of Croatia, where the ethnicization of religion, beginning in the early 1970s, was rather pronoun-ced, relations between the Catholic Church and state authorities in Slovenia – the northernmost republic of Yugoslavia – remained relatively calm and cooperative throughout the late socialist period. Based on an analysis of a wide range of pu-blic and archival documents, this paper demonstrates how Slovenian religious policy was proposed as a sophisticated example of how believers could be success-fully integrated into modern socialist society, and was presented as such to Vati-can diplomats, international experts, and foreign journalists. Up until 1990, the communication between party officials and the Church hierarchy conveyed a di-stinct tone of courtesy, and local priests generally encountered a supportive or at least unobstructive attitude when, for instance, proposing the construction of new churches. At the same time, however, the more independent intellectuals, Catholi-cs and Marxists alike, who urged the party to abandon its orthodox Marxist-Leni-nist understanding of religion in favour of a genuine dialogue, were marginalized. Thus, a proper debate about a topic so essential for socialist secular society never took place, and the late socialist religious policy left behind an ambiguous legacy.
Ključne besede: Church-State relations, Yugoslavia, Slovenia, self-management socialism, civil rights of believers
Objavljeno v DiRROS: 05.08.2025; Ogledov: 330; Prenosov: 197
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