61. Transcription factors gene expression in chronic rhinosinusitis with and without nasal polypsTanja Soklič, Matija Rijavec, Mira Šilar, Ana Koren, Izidor Kern, Irena Hočevar-Boltežar, Peter Korošec, 2019, izvirni znanstveni članek Povzetek: Background. Chronic rhinosinusitis (CRS) current therapeutic approaches still fail in some patients with severe persistent symptoms and recurrences after surgery. We aimed to evaluate the master transcription factors gene expression levels of T cell subtypes in chronic rhinosinusitis with nasal polyps (CRSwNP) and chronic rhinosinusitis without nasal polyps (CRSsNP) that could represent new, up-stream targets for topical DNAzyme treatment. Patients and methods. Twenty-two newly diagnosed CRS patients (14 CRSwNP and 8 CRSsNP) were prospectively biopsied and examined histopathologically. Gene expression levels of T-box transcription factor (T-bet, TBX21), GATA binding protein 3 (GATA3), Retinoic acid-related orphan receptor C (RORC) and Forkhead box P3 (FOXP3) were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). Results. Eosinophilic CRSwNP was characterized by higher level of GATA3 gene expression compared to noneosinophilic CRSwNP, whereas there was no difference in T-bet, RORC and FOXP3 between eosinophilic and noneosinophilic CRSwNP. In CRSsNP, we found simultaneous upregulation of T-bet, GATA3 and RORC gene expression levels in comparison to CRSwNP; meanwhile, there was no difference in FOXP3 gene expression between CRSwNP and CRSsNP. Conclusions. In eosinophilic CRSwNP, we confirmed the type 2 inflammation by elevated GATA3 gene expression level. In CRSsNP, we unexpectedly found simultaneous upregulation of T-bet and GATA3 that is currently unexplained; however, it might originate from activated CD8+ cells, abundant in nasal mucosa of CRSsNP patients. The elevated RORC in CRSsNP could be part of homeostatic nasal immune response that might be better preserved in CRSsNP patients compared to CRSwNP patients. Further data on transcription factors expression rates in CRS phenotypes are needed.
Ključne besede: sinusitis, nasal polyps, Th1 cells, Th2 cells, Th17 cells, transcription factors, chronic rhinosinusitis
Objavljeno v DiRROS: 09.10.2020; Ogledov: 1545; Prenosov: 840
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62. Heritable risk for severe anaphylaxis associated with increased [alpha]-tryptase-encoding germline copy number at TPSAB1Jonathan J. Lyons, Jack Chovanec, Michael P. O'Connell, Yihui Liu, Julij Šelb, Roberta Zanotti, Yun Bai, Jiwon Kim, Quang T. Le, Tom DiMaggio, Matija Rijavec, Peter Korošec, 2020, izvirni znanstveni članek Povzetek: Background: An elevated basal serum tryptase level is associated with severe systemic anaphylaxis, most notably caused by Hymenoptera envenomation. Although clonal mast cell disease is the culprit in some individuals, it does not fully explain this clinical association. Objective: Our aim was to determine the prevalence and associated impact of tryptase genotypes on anaphylaxis in humans. Methods: Cohorts with systemic mastocytosis (SM) and venom as well as idiopathic anaphylaxis from referral centers in Italy, Slovenia, and the United States, underwent tryptase genotyping by droplet digital PCR. Associated anaphylaxis severity (Mueller scale) was subsequently examined. Healthy volunteers and controls with nonatopic disease were recruited and tryptase was genotyped by droplet digital PCR and in silico analysis of genome sequence, respectively. The effects of pooled and recombinant human tryptases, protease activated receptor 2 agonist and antagonist peptides, and a tryptase-neutralizing mAb on human umbilical vein endothelial cell permeability were assayed using a Transwell system. Results: Hereditary [alpha]-tryptasemia (H[alpha]T)--a genetic trait caused by increased [alpha]-tryptase-encoding Tryptase-[alpha]/[beta]1 (TPSAB1) copy number resulting in elevated BST level--was common in healthy individuals (5.6% [n = 7 of 125]) and controls with nonatopic disease (5.3% [n = 21 of 398]). H[alpha]T was associated with grade IV venom anaphylaxis (relative risk = 2.0; P < .05) and more prevalent in both idiopathic anaphylaxis (n = 8 of 47; [17%; P = .006]) and SM (n = 10 of 82 [12.2%; P = .03]) relative to the controls. Among patients with SM, concomitant H[alpha]T was associated with increased risk for systemic anaphylaxis (relative risk = 9.5; P = .007). In vitro, protease-activated receptor-2-dependent vascular permeability was induced by pooled mature tryptases but not [alpha]- or [beta]-tryptase homotetramers. Conclusions: Risk for severe anaphylaxis in humans is associated with inherited differences in [alpha]-tryptase-encoding copies at TPSAB1.
Ključne besede: mastocytosis, venoms, hypersensitivity, anaphylaxis - diagnosis, mast cells, idiopathic anaphylaxis, mast cell activation, hereditary alpha-tryptasemia
Objavljeno v DiRROS: 11.09.2020; Ogledov: 2056; Prenosov: 380
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63. Poškodbe drevja zaradi abiotskih naravnih motenj na bukovih rastiščih v Sloveniji s poudarkom na snegolomihBlanka Klinar, Matija Klopčič, Andrej Bončina, 2020, izvirni znanstveni članek Povzetek: Raziskovali smo poškodovanost drevja zaradi snega v pretežno mešanih gozdnih sestojih na bukovih rastiščih v Sloveniji. V analizo smo vključili 22.609 oddelkov s prevladujočimi bukovimi rastišči, v katerih je bil delež bukve vsaj 10 % lesne zaloge sestojev. Delež iglavcev v sanitarnem poseku je bil večji pri snegolomih in vetrolomih, pri žledolomih sta bila deleža listavcev in iglavcev skoraj enaka. V bukovih gozdovih je zaradi snega najbolj poškodovano drevje v mlajših in srednjedobnih razvojnih fazah debelin 20%40 cm. Na podvzorcu 363 oddelkov, za katere smo pridobili podatke o lastnostih snežne odeje, smo z binarno logistično regresijo izdelali model verjetnosti pojava snegoloma. V model smo vključili spremenljivke količine snega v spomladanskih mesecih, fitoregijo, skalnatost, naklon terena in matično podlago. Najpomembnejša spremenljivka je bila količina snega v spomladanskih mesecih. Razlog za relativno visok sanitarni posek v bukovih gozdovih je povečan delež smreke v gozdnih sestojih, bukev je veliko manj dovzetna za poškodbe zaradi snega kot smreka. Za povečanje odpornosti sestojev predlagamo pogostejša klasična redčenja nižjih jakosti ali uvedbo situacijskih redčenj v sestojih mlajših in srednjedobnih sestojih.
Ključne besede: Fagus sylvatica, bukova rastišča, binarna logistična regresija, pojav poškodbe, snegolomi, naravne motnje, gozdovi
Objavljeno v DiRROS: 09.09.2020; Ogledov: 5337; Prenosov: 1655
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64. Unsuccessful desensitization to paclitaxel in a patient with high basophil sensitivityPeter Kopač, Ana Koren, Maja Jošt, Dushan Mangaroski, Mitja Lainščak, Peter Korošec, 2020, drugi znanstveni članki Ključne besede: basophil activation test, drug hypersensitivity, immunologic desensitization, paclitaxel
Objavljeno v DiRROS: 07.08.2020; Ogledov: 1704; Prenosov: 492
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65. Heat shock protein 27 as a predictor of prognosis in patients admitted to hospital with acute COPD exacerbationMatthias Zimmermann, Denise Traxler, Christine Bekos, Elisabeth Simader, Thomas Mueller, Alexandra Graf, Mitja Lainščak, Robert Marčun, Mitja Košnik, Matjaž Fležar, Aleš Rozman, Peter Korošec, 2020, izvirni znanstveni članek Povzetek: Episodes of acute exacerbations are major drivers of hospitalisation and death from COPD. To date, there are no objective biomarkers of disease activity or biomarkers to predict patient outcome. In this study, 211 patients hospitalised for an acute exacerbation of COPD have been included. At the time of admission,routine blood tests have been performed including complete blood count, C-reactive protein, cardiac troponin T and NT-proBNP. Heat shock protein 27 (HSP27) serum concentrations were determined at time of admission, discharge and 180 days after discharge by ELISA. We were able to demonstrate significantly increased HSP27 serum concentrations in COPD patients at time of admission to hospital as compared to HSP27 concentrations obtained 180 days after discharge. In univariable Cox regression analyses, a HSP27 serum concentration >/= 3098 pg/mL determined at admission was a predictor of all-cause mortality at 90 days, 180 days, 1 year and 3 years. In multivariable analyses, an increased HSP27 serum concentration at admission retained its prognostic ability with respect to all-cause mortality for up to 1year follow-up. However, an increased HSP27 serum concentration at admission was not an independent predictor of long-term all-cause mortality at 3 years. Elevated serum HSP27 concentrations significantly predicted short-term mortality in patients admitted to hospital with acute exacerbation of COPD and could help to improve outcomes by identifying high-risk patients.
Ključne besede: COPD, acute exacerbation, disease activity
Objavljeno v DiRROS: 29.07.2020; Ogledov: 1739; Prenosov: 1071
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66. Smernice za obravnavo bolnic z rakom jajčnikov, jajcevodov in s primarnim peritonealnim seroznim rakomMatija Barbič, Sonja Bebar, Olga Cerar, Branko Cvjetičanin, Janka Čarman, Astrid Djurišić, Snježana Frković-Grazio, Barbara Gazić, Andreja Gornjec, Katja Jakopič, Borut Kobal, Mateja Krajc, Sebastjan Merlo, Maja Marolt-Mušič, Maja Pakiž, Špela Smrkolj, Manja Šešek, Erik Škof, Iztok Takač, Aleš Vakselj, Vesna Zadnik, Helena Barbara Zobec Logar, 2015, strokovna monografija Ključne besede: zdravstvena obravnava, smernice, rak jajčnikov, ginekologija, neoplazme, ginekološki raki
Objavljeno v DiRROS: 09.06.2020; Ogledov: 1787; Prenosov: 557
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68. Postopki za obravnavo bolnic z rakom jajčnikov, jajcevodov in s primarnim peritonealnim seroznim rakomMatija Barbič, Sonja Bebar, Olga Cerar, Branko Cvjetičanin, Janka Čarman, Astrid Djurišić, Snježana Frković-Grazio, Barbara Gazić, Andreja Gornjec, Katja Jakopič, Borut Kobal, Mateja Krajc, Sebastjan Merlo, Maja Marolt-Mušič, Maja Pakiž, Špela Smrkolj, Manja Šešek, Erik Škof, Iztok Takač, Aleš Vakselj, Vesna Zadnik, Helena Barbara Zobec Logar, 2015, strokovna monografija Ključne besede: zdravstvena obravnava, rak jajčnikov, ginekologija, neoplazme, ginekološki raki
Objavljeno v DiRROS: 27.05.2020; Ogledov: 1938; Prenosov: 585
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69. Smernice za obravnavo bolnic z rakom jajčnikov, jajcevodov in s primarnim peritonealnim seroznim rakomHelena Barbara Zobec Logar, Špela Smrkolj, Sebastjan Merlo, Sonja Bebar, Borut Kobal, Erik Škof, Olga Cerar, Branko Cvjetičanin, Aleš Vakselj, Manja Šešek, Janka Čarman, Astrid Djurišić, Barbara Gazić, Andreja Gornjec, Katja Jakopič, Mateja Krajc, Maja Marolt-Mušič, Vesna Zadnik, Matija Barbič, Iztok Takač, Maja Pakiž, 2016, strokovna monografija Ključne besede: zdravstvena obravnava, smernice
Objavljeno v DiRROS: 27.05.2020; Ogledov: 2087; Prenosov: 520
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70. Robinija (Robinia pseudoacacia L.) v Beli krajini: razširjenost, priraščanje, pomlajevanje in upravljanjeBlaž Bahor, Matija Klopčič, 2019, izvirni znanstveni članek Povzetek: Raziskovali smo razširjenost, priraščanje in pomlajevanje robinije v Beli krajini. Uporabili smo podatke Zavoda za gozdove Slovenije, del podatkov o pomlajevanju smo dodatno posneli. S podatki o gozdnih sestojih smo izračunali delež robinije v lesni zalogi in izdelali karto razširjenosti robinije ter karto površin, pomlajenih z robinijo. Debelinski in volumenski prirastek robinij smo izračunali s pomočjo podatkov s stalnih vzorčnih ploskev ter ju primerjali s prirastki drugih drevesnih vrst. S terensko pridobljenimi podatki smo analizirali pomladek na naključno izbranih stalnih vzorčnih ploskvah. Ugotovili smo, da se robinija pojavlja na 6,6 % gozdne površine, obilneje ob gozdnih robovih zunaj strnjenih gozdnih kompleksov. Lesna zaloga robinije sestavlja 3,5 % celotne lesne zaloge gozdov. Debelinski in volumenski prirastek robinije je višji od prirastka večine drevesnih vrst, s katerimi se najpogosteje pojavlja v sestojih. V prihodnosti je pričakovati nadaljnje širjenje robinije, zato jo je treba ustrezno upoštevati pri gospodarjenju z gozdovi.
Ključne besede: robinija, razširjenost, priraščanje, pomlajevanje, Bela krajina
Objavljeno v DiRROS: 29.11.2019; Ogledov: 6798; Prenosov: 2259
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