1801. Risk factors for severe sting reactions and side effects during venom immunotherapyGunter Sturm, Eva Schadelbauer, Giorgia Marta, Patrizia Bonadonna, Mitja Košnik, 2025, pregledni znanstveni članek Povzetek: Understanding the risk factors leading to severe systemic sting reactions (SSRs) is crucial for initiating venom immunotherapy (VIT) and for educating affected individuals and their families. Some of these risk factors are well established, some are no longer considered risk factors, and some remain controversial. Well-established risk factors for severe SSRs include clonal mast cell disease, high baseline serum tryptase, and advanced age. The absence of skin symptoms and the rapid onset of symptoms are indicators of severe SSRs. Recent publications indicate that antihypertensive treatment and stings in the head and neck area are not risk factors for severe SSRs. VIT is the only available treatment that can potentially prevent further anaphylactic reactions. Although rare and generally manageable, individuals undergoing VIT may experience systemic adverse events (sAEs). More sAEs are expected in patients undergoing bee VIT compared with vespid VIT. The role of elevated baseline serum tryptase as a risk factor for sAEs remains debated, but if it is a factor, the risk is increased by only about 1.5-fold. Rapid updosing protocols, depending on the specific regimen, can also be associated with more sAEs. Severe initial SSRs, antihypertensive medication, high skin test reactivity, and high specific IgE levels are not risk factors for sAEs.
Ključne besede: immunology, anaphylaxis, Hymenoptera venom allergy, risk factors, severe systemic sting reactions, side effects, venom immunotherapy
Objavljeno v DiRROS: 02.07.2025; Ogledov: 465; Prenosov: 379
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1802. Usage prevalence of angioedema patient-reported outcome measures : results from the UCARE and ACARE PROMUSE studyIvan Cherrez-Ojeda, Jean Bousquet, Ana M. Giménez-Arnau, Kiran V Godse, Dorota Krasowska, Joanna Bartosińska, Paulina Szczepanik-Kułak, Bartłomiej Wawrzycki, Pavel Kolkhir, Anastasiia Allenova, Mitja Košnik, 2024, drugi znanstveni članki Ključne besede: allergy, angioedema, measures, results of tests, patient reported outcome measures
Objavljeno v DiRROS: 02.07.2025; Ogledov: 410; Prenosov: 386
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1803. Combination of experimental and bioinformatic approaches for identification of immunologically relevant protein–peptide InteractionsJerneja Debeljak, Peter Korošec, Julij Šelb, Matija Rijavec, Mitja Košnik, Mojca Lunder, 2023, izvirni znanstveni članek Povzetek: Protein–peptide interactions are an essential player in cellular processes and, thus, of great interest as potential therapeutic agents. However, identifying the protein’s interacting surface has been shown to be a challenging task. Here, we present a methodology for protein–peptide interaction identification, implementing phage panning, next-generation sequencing and bioinformatic analysis. One of the uses of this methodology is identification of allergen epitopes, especially suitable for globular inhaled and venom allergens, where their binding capability is determined by the allergen’s conformation, meaning their interaction cannot be properly studied when denatured. A Ph.D. commercial system based on the M13 phage vector was used for the panning process. Utilization of various bioinformatic tools, such as PuLSE, SAROTUP, MEME, Hammock and Pepitope, allowed us to evaluate a large amount of obtained data. Using the described methodology, we identified three peptide clusters representing potential epitopes on the major wasp venom allergen Ves v 5.
Ključne besede: phage panning, next-generation sequencing, bioinformatic analysis, allergen Ves v 5, epitopes
Objavljeno v DiRROS: 02.07.2025; Ogledov: 417; Prenosov: 383
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1805. Programmable protein-DNA composite nanostructures : from nanostructure construction to protein-induced micro-scale material self-assembly and functionalizationWeijun Zhou, Žiga Strmšek, Jaka Snoj, Miha Škarabot, Roman Jerala, 2025, izvirni znanstveni članek Povzetek: The integration of DNA and protein-designed nanostructures represents a transformative approach to the development of programmable biopolymers for nanoscale construction. While DNA nanostructures excel in the readily programmable precision and scalability of base pairing, protein assemblies exploit the chemical diversity of amino acids for greater functional versatility. Here a platform is presented that unifies these two paradigms by combining coiled-coil protein origami with DNA nanostructures through orthogonal protein-protein (SpyCatcher-SpyTag) and protein-DNA (DCV-DNA) covalent conjugation strategies. This dual-functionalization strategy enables the construction of stable and versatile protein-DNA composites capable of hierarchical self-assembly. This shows that these composites drive the transformation of DNA nanotubes into large-scale, patterned nanofibers or nanorods, with the proteins regularly distributed over their surface and retaining their enzymatic and fluorescent functions. In addition, a DNA-luciferase circuit is developed through split enzyme reconstitution to achieve reversible regulation of enzymatic activity, highlighting the dynamic functionality of these composites. This introduces a modular approach to producing multifunctional bio-nanomaterials, highlighting the potential of protein-DNA composite nanostructures as a bridge between molecular design and functional nanomaterials and paves the way for the development of dynamic bio-devices and programmable biomaterials.
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1806. Anti-icing performance and anti-corrosive properties of superhydrophobic nanosecond-laser-textured aluminum surfaces with a self-assembled monolayer coatingMatic Može, Peter Rodič, Tim Štrus, Miha Štucin, Robert Lovšin, Nina Kovač, Samo Jereb, Armin Hadžić, Matevž Zupančič, Iztok Golobič, 2025, izvirni znanstveni članek Povzetek: Water and ice accumulation on surfaces present significant challenges in numerous fields, causing issues such as corrosion of metallic surfaces and a broad range of problems associated with increased weight, altered hydro-/aerodynamics, vibrations, and heat transfer resistance. Currently, passive solutions, such as functionalized surfaces that also offer improved anti-corrosive properties, are being focused on. This study presents the fabrication and evaluation of four functionalized superhydrophobic surfaces on 1050A aluminum alloy samples using nanosecond laser texturing and self-assembled monolayer grafting to enhance anti-icing and corrosion protection. The developed surfaces exhibited superhydrophobicity with static and dynamic contact angles above 163° and a contact angle hysteresis below 2°, maintaining their nonwetting properties after multiple icing/deicing cycles. Three surfaces significantly lowered ice nucleation temperature with the lowest average value of -20.1 °C recorded on the surface with shallow, non-distinct features. The freezing delay was prolonged up to 468 % and 944 % at -15 °C and -20 °C, respectively, in comparison with the untreated reference surface. The superior anti-icing properties of the two surfaces with shallow structures, fabricated using a lower laser pulse fluence than those with deep structures, are ascribed to a lower number of potential active nucleation sites for condensation of water vapor and freezing initiation. In comparison with an untreated surface, ice adhesion was reduced by up to 60 % on surfaces with shallow features but increased between 33 % and 84 % on surfaces with deep morphological features. Repeatable cascade freezing was observed on one of the surfaces, which also exhibited the poorest anti-icing performance. Surfaces with non-distinct or irregular channels exhibited better corrosion properties than surfaces featuring well-defined laser-etched channels. The surface with the lowest surface roughness exhibited the best properties in all evaluations, providing a good starting point for future optimization with a focus on surfaces with micron scale and submicron features instead of the more traditional surfaces with structures on the scale of tens of microns. Overall, this functionalization approach is promising for industrial use, simultaneously offering enhanced robustness, anti-icing behavior, and corrosion protection.
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1809. Poročilo o spremljanju stanja gozdov za leto 2024 : vsebinsko poročilo o spremljanju stanja gozdov v skladu s Pravilnikom o varstvu gozdov (2009)Anže Martin Pintar, Pia Höfferle, Janez Kermavnar, Nike Krajnc, Lado Kutnar, Tom Levanič, Gal Oblišar, Nikica Ogris, Matej Rupel, Primož Simončič, Saša Šercer, Urša Vilhar, Daniel Žlindra, 2025, končno poročilo o rezultatih raziskav Ključne besede: varstvo gozdov, monitoring, stanje gozdov, zdravstveno stanje krošenj, popis povzročiteljev poškodb, meteorološke meritve, osutost dreves, poročila, Slovenija
Objavljeno v DiRROS: 01.07.2025; Ogledov: 863; Prenosov: 327
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1810. Poslovanje slovenskega gradbeništva v zadnjem desetletjuBojan Ivanc, 2025, izvirni znanstveni članek Povzetek: Dinamika poslovanja v gradbeništvu je v zadnjem desetletju dosegla lokalno dno v letu 2016, potem pa je pričela močno naraščati. Leti 2022 in 2023 sta bili po rasti izjemni leti, predvsem zaradi rasti del, financiranih iz kohezijskih sredstev in Načrta za okrevanje in odpornost. Leto 2024 je bilo spet nekoliko šibkejše, predvsem zaradi padca gradbenih del, kar je bilo povezano z zamudami pri izdaji gradbenih dovoljenj na upravnih enotah. Rast gradbenih del je sovpadala z rastjo števila gradbenih podjetij, katerih povprečna velikost, merjena s številom zaposlenih, je sčasoma vseeno nekoliko porasla. Dinamika črpanja kohezijskih sredstev je pomembno vplivala na dinamiko poslovanja v gradbeništvu. Gradbeništvo je predstavljalo okoli 6 % ustvarjene dodane vrednosti v slovenskem gospodarstvu, s čimer se je slovensko gradbeništvo uvrstilo na enajsto mesto med 27 EU državami. Prejemki zaposlenih so v zadnjem desetletju naraščali hitreje kot v povprečju in so bili v letu 2024 le še za desetino nižji od slovenskega povprečja. Kljub visoki rasti plač se je z izzivom pomanjkanja usposobljenih delavcev v zadnjih treh letih (2022-2024) soočala že polovica gradbenih podjetij. To zelo verjetno vpliva na nižjo produktivnost dela in na visoko rast plač oziroma stroškov dela na zaposlenega. Zaradi visokega deleža stroškov dela v dodani vrednosti gradbena podjetja ne morejo dodatno dvigovati plač, kar bi prispevalo k zmanjševanju primanjkljaja ponudbe delovne sile
Ključne besede: gradbena dela, dovoljenja, dodana vrednost, kazalnik zaupanja, omejitveni dejavniki
Objavljeno v DiRROS: 01.07.2025; Ogledov: 322; Prenosov: 160
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