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Abstract: One of the key steps of the molecular identification of bark beetles is obtaining a sufficient quantity of high-quality DNA extract. In this study, we investigated the influence of different storage procedures for Ips typographus (L.) specimens and various DNA extraction protocols on the quantity and quality of DNA intended for use in molecular diagnostics. Adult beetles were frozen at -20 °C, either dry or in ethanol. We tested four different protocols for DNA extraction. We compared the quantity of extracted DNA and assessed its quality with PCR and Sanger sequencing. Different storage protocols had no significant effect on the quantity of DNA extracted. However, freezing specimens in ethanol provided higher-quality DNA for molecular applications. Only two of the extraction protocols produced sequenceable amplicons, and the difference in the amount of extracted DNA between them was not significant. We propose the optimal combination of storing specimens in ethanol at -20°C and using the Nucleospin Insect DNA extraction kit from Macherey Nagel, enabling a timeefficient identification process.
Keywords: early detection, specimen storage, total DNA extraction, PCR, polymerase chain reaction, Sanger sequencing, molecular diagnostics
Published in DiRROS: 02.02.2024; Views: 711; Downloads: 257
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Abstract: Invasive alien species (IAS) adversely impact biodiversity, ecosystem functions and socio-economics. Citizen science can be an effective tool for IAS surveillance, management and research, providing large datasets over wide spatial extents and long time periods, with public participants generating knowledge that supports action. We demonstrate how citizen science has contributed knowledge across the biological invasion process, especially for early detection and distribution mapping. However, we recommend that citizen science could be used more for assessing impacts and evaluating the success of IAS management. Citizen science does have limitations and we explore solutions to two key challenges: ensuring data accuracy and dealing with uneven spatial coverage of potential recorders (which limits the dataset’s ‘fit for purpose’). Greater co-development of citizen science with public stakeholders will help us better realise its potential across the biological invasion process and across ecosystems globally while meeting the needs of participants, local communities, scientists and decision-makers.
Keywords: biosecurity, community science, invasion continuum, invasive alien species, monitoring, volunteers
Published in DiRROS: 25.01.2024; Views: 397; Downloads: 227
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Abstract: Članek prikazuje rezultate raziskave razširjenosti vrste Ips duplicatus (Sahlberg, 1836) v Sloveniji v letu 2023. Uporabili smo 14 režastih pasti tipa Theysohn, ki so bile opremljene s feromonom Dupliwit. V raziskavo smo vključili tudi tri križne prestrezne pasti brez feromona, ki so bile postavljene v sklopu drugega projekta in smo v njih odkrili vrsto I. duplicatus. Prisotnost vrste smo potrdili na šestih lokacijah, skupno pa smo ujeli osem osebkov. Predstavljena je razprava o pomenu teh najdb v kontekstu razširjenosti, spremljanja in upravljanja dvojnozobega smrekovega lubadarja.
Keywords: gozdovi, varstvo gozdov, monitoring, navadna smreka, Picea abies, podlubniki, invazivna tujerodna vrsta
Published in DiRROS: 10.01.2024; Views: 374; Downloads: 153
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Keywords: varstvo gozdov, morfološke analize, Ips duplicatus, dvojnozobi smrekov lubadar
Published in DiRROS: 19.12.2023; Views: 357; Downloads: 0

Abstract: The Environmental Impact Classification for Alien Taxa (EICAT) is an important tool for biological invasion policy and management and has been adopted as an International Union for Conservation of Nature (IUCN) standard to measure the severity of environmental impacts caused by organisms living outside their native ranges. EICAT has already been incorporated into some national and local decision-making procedures, making it a particularly relevant resource for addressing the impact of non-native species. Recently, some of the underlying conceptual principles of EICAT, particularly those related to the use of the precautionary approach, have been challenged. Although still relatively new, guidelines for the application and interpretation of EICAT will be periodically revisited by the IUCN community, based on scientific evidence, to improve the process. Some of the criticisms recently raised are based on subjectively selected assumptions that cannot be generalized and may harm global efforts to manage biological invasions. EICAT adopts a precautionary principle by considering a species’ impact history elsewhere because some taxa have traits that can make them inherently more harmful. Furthermore, non-native species are often important drivers of biodiversity loss even in the presence of other pressures. Ignoring the precautionary principle when tackling the impacts of non-native species has led to devastating consequences for human well-being, biodiversity, and ecosystems, as well as poor management outcomes, and thus to significant economic costs. EICAT is a relevant tool because it supports prioritization and management of non-native species and meeting and monitoring progress toward the Kunming–Montreal Global Biodiversity Framework (GBF) Target 6.
Keywords: biological invasions, evidence synthesis, impact assessment, managing invasive species, precautionary principle
Published in DiRROS: 11.12.2023; Views: 473; Downloads: 279
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