| Title: | Immunophenotyping, activation, and expansion of gamma delta T cells: flow cytometry profiling and ex vivo manipulation strategies |
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| Authors: | ID Rupar, Urška (Author)
ID Bertoncelj, Pia (Author)
ID Leben Zupet, Katja (Author)
ID Markelj, Gašper (Author)
ID Vesel, Tina (Author)
ID Šamec, Neja (Author)
ID Avčin, Tadej (Author)
ID Pohar, Jelka (Author)
ID Smole, Anže (Author) |
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| Files: |  URL - Source URL, visit https://doi.org/10.3389/fimmu.2026.1829178
 PDF - Presentation file, download (1,57 MB)
MD5: F7137F15E2B0BF36EE3AC68022E16906
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Gamma delta (γδ) T cells exhibit unique antiviral, antibacterial, and antitumor properties. Advancing the fundamental understanding of γδ T cell immunobiology and unlocking their full therapeutic potential requires detailed immunophenotypic profiling alongside optimized strategies for ex vivo activation and expansion. In this review, we synthesize current knowledge of γδ T cell immunobiology, emphasizing (i) comprehensive phenotypic characterization using flow cytometry and (ii) emerging approaches for their therapeutic implementation, with a focus on activation and ex vivo expansion methods. By integrating insights from phenotypic analysis with a comparative evaluation of expansion techniques, we provide an overview of γδ T cell immunobiology and their translational potential in immunotherapy. |
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| Keywords: | barrier immunity, cellular immunotherapy, flow cytometry, gamma delta (γδ) T cells, immune dysregulation, immunophenotyping, γδ T cell activation, γδ T cell expansion, immunology |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 31.07.2026 |
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| Year of publishing: | 2026 |
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| Number of pages: | 1-14 str. |
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| Numbering: | Vol. 17 |
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| PID: | 20.500.12556/DiRROS-31461  |
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| UDC: | 616-097 |
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| ISSN on article: | 1664-3224 |
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| DOI: | 10.3389/fimmu.2026.1829178 |
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| COBISS.SI-ID: | 286715651 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 3. 8. 2026;
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| Publication date in DiRROS: | 03.08.2026 |
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| Views: | 31 |
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| Downloads: | 21 |
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