20.500.12556/DiRROS-31461 Immunophenotyping, activation, and expansion of gamma delta T cells: flow cytometry profiling and ex vivo manipulation strategies Gamma delta (γδ) T cells exhibit unique antiviral, antibacterial, and antitumor properties. Advancing the fundamental understanding of γδ T cell immunobiology and unlocking their full therapeutic potential requires detailed immunophenotypic profiling alongside optimized strategies for ex vivo activation and expansion. In this review, we synthesize current knowledge of γδ T cell immunobiology, emphasizing (i) comprehensive phenotypic characterization using flow cytometry and (ii) emerging approaches for their therapeutic implementation, with a focus on activation and ex vivo expansion methods. By integrating insights from phenotypic analysis with a comparative evaluation of expansion techniques, we provide an overview of γδ T cell immunobiology and their translational potential in immunotherapy. barrier immunity cellular immunotherapy flow cytometry gamma delta (γδ) T cells immune dysregulation immunophenotyping γδ T cell activation γδ T cell expansion immunology celična imunoterapija pretočna citometrija celice γδ T imunska disregulacija imunofenotipizacija aktivacija celic γδ T ekspanzija celic γδ T true false true Angleški jezik Slovenski jezik Neznano 2026-08-03 11:28:37 2026-08-03 11:28:37 2026-08-04 03:53:12 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Opis vira z dne 3. 8. 2026; 1-14 str. Vol. 17 Jul. 2026 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2026-07-31 616-097 1664-3224 10.3389/fimmu.2026.1829178 286715651 https://doi.org/10.3389/fimmu.2026.1829178 1 b7cce5f5-8f1d-11f1-a7fa-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=48739 RAZ_Rupar_Urska_2026.pdf RAZ_Rupar_Urska_2026.pdf 1 F7137F15E2B0BF36EE3AC68022E16906 2a06d6d2d34bd375c170c813b26bd5a38d98b6a40aa394c38227c3ef7d0b18ee 420a899c-8f1e-11f1-a7fa-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=48741 Nacionalni inštitut za biologijo 0 0 0