| Title: | In vitro genotoxicity assessment of graphene quantum dots using a 3D HepG2 model : version v1 |
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| Authors: | ID Durmišević, Irma (Author)
ID Haverić, Anja (Author)
ID Kološa, Katja (Author)
ID Žegura, Bojana (Author) |
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| Files: |  URL - Source URL, visit https://zenodo.org/records/18415123
 DOCX - Research data, download (26,18 KB)
MD5: D520AC026E3FF358179B70DAE975BEA6
 JSON - Metadata, download (4,59 KB)
MD5: B177FF5617D223969E3C32F9A4B5D067
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| Language: | English |
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| Typology: | 2.20 - Complete scientific database of research data |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Graphene quantum dots (GQDs) are nano-sized fragments of graphene sheets, with great potential for applications in optics, electrochemistry, and biomedicine, including drug delivery. In this study, two types of GQDs were investigated: green-emitting (G-GQD) and blue-emitting (B-GQD) and their genotoxic potential was examined on the protein level. Flow cytometric analysis of γH2AX, a marker of DNA double-strand breaks, and phosphorylated histone H3 (p-H3), a marker of aneugenic activity, was performed in HepG2 spheroids after 24 hours of exposure to G-GQDs and B-GQDs. HepG2 spheroids were exposed to graded concentrations of B- and G-GQD (12.5, 25, 50, and 100 μg/mL) for 24 hours, respectively. Spheroids were dissociated into single-cell suspensions, washed twice with 1x PBS and fixed in 4% PFA. For flow cytometric analysis, cells were labelled with anti-H2AX pS139 antibody and anti-histone H3 pS128 antibody. REA-APC and REA-PE controls were applied to exclude non-specific antibody binding. For each sample, 10,000 events were acquired using a MACSQuant Analyzer 10 flow cytometer and MACSQuantify™ software (Miltenyi Biotech, Germany). Raw data were exported from the MACSQuantify software and analysed with FlowJo V10 software (Becton Dickinson, New Jersey, USA). |
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| Keywords: | nanomaterials, graphene quantum dots, HepG2 spheroids, toxicity |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 29.01.2026 |
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| Place of publishing: | Genève |
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| Place of performance: | Genève |
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| Publisher: | Zenodo |
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| Year of publishing: | 2026 |
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| Year of performance: | 2026 |
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| Number of pages: | 1 spletni vir |
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| PID: | 20.500.12556/DiRROS-31433  |
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| UDC: | 579 |
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| DOI: | 10.5281/zenodo.18415123 |
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| COBISS.SI-ID: | 280617475 |
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| Note: | Nasl. z. nasl. zaslona;
Opis vira z dne 5. 6. 2026;
Soavtorji: Haverić, Anja; Kološa, Katja; Zegura, Bojana;
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| Publication date in DiRROS: | 31.07.2026 |
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| Views: | 43 |
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| Downloads: | 31 |
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| Metadata: |    |
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