| Title: | Snake venom c-type lectin-like protein Vaa-snaclec-3/2 efficiently prevents carotid artery thrombosis in a mouse model without compromising blood coagulation |
|---|
| Authors: | ID Žužek, Monika C. (Author)
ID Križaj, Igor (Author)
ID Brvar, Miran (Author)
ID Trobec, Tomaž (Author)
ID Kranjc Brezar, Simona (Author)
ID Dobaja, Mojca (Author)
ID Leonardi, Adrijana (Author)
ID Požek, Kity (Author)
ID Vrecl, Milka (Author)
ID Frangež, Robert (Author) |
|---|
| Files: |  PDF - Presentation file, download (7,95 MB)
MD5: 871AC60620B902304C4084D2D90D5F8D
 URL - Source URL, visit https://www.mdpi.com/2072-6651/17/11/523
|
|---|
| Language: | English |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: |  UKC LJ - Ljubljana University Medical Centre
|
|---|
| Abstract: | Platelets play pivotal roles in thromboembolic diseases, such as myocardial infarction and ischemic stroke. In patients envenomed by the snake Vipera a. ammodytes (Vaa), pronounced and transient thrombocytopenia without bleeding is observed. We previously showed that Vaa-snaclec-3/2, the snake venom C-type lectin-like protein, mediates this effect ex vivo. Here, we extended our study of the antithrombotic potential of this protein in vivo using a mouse model of ferric chloride (FeCl3)-induced carotid artery thrombosis. Prior to inducing thrombus formation, the mice received 1, 5, 10, 20, or 50 μg/kg Vaa-snaclec-3/2 intravenously. Afterward, the arterial blood flow was monitored with a perivascular Doppler probe. Additionally, the platelet count in the peripheral venous blood; tail bleeding time; and liver, lung, kidney, spleen, and heart histology were evaluated. The lowest dose of Vaa-snaclec-3/2 that we showed to cause severe thrombocytopenia and completely inhibit FeCl3-induced thrombus formation was 20 µg/kg. This dose prolonged the median tail bleeding time from 86.5 to 153.5 s but did not induce acute spontaneous hemorrhage, as demonstrated by histological analysis. Histology revealed no signs of apoptosis, necrosis or other degenerative changes in the inspected organs of mice exposed to 20 μg/kg Vaa-snaclec-3/2. Platelet clusters were observed only in the lungs, which appear to be the primary site of platelet sequestration and the cause of thrombocytopenia. Taken together, our findings highlight the high potential of Vaa-snaclec-3/2 as a safe and effective antithrombotic agent for the transient prevention of thrombosis in acute clinical settings. |
|---|
| Keywords: | snake venom, antithrombotic, snaclec, arterial thrombosis, mice |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Year of publishing: | 2025 |
|---|
| Number of pages: | str. 1-14 |
|---|
| Numbering: | Vol. 17, issue 11, [article no.] 523 |
|---|
| PID: | 20.500.12556/DiRROS-29072  |
|---|
| UDC: | 615:616-092:636.09 |
|---|
| ISSN on article: | 2072-6651 |
|---|
| DOI: | 10.3390/toxins17110523 |
|---|
| COBISS.SI-ID: | 254604035 |
|---|
| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 24. 10. 2025;
|
|---|
| Publication date in DiRROS: | 20.04.2026 |
|---|
| Views: | 190 |
|---|
| Downloads: | 162 |
|---|
| Metadata: |    |
|---|
|
:
|
Copy citation |
|---|
| | | | Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
