20.500.12556/DiRROS-29072 Snake venom c-type lectin-like protein Vaa-snaclec-3/2 efficiently prevents carotid artery thrombosis in a mouse model without compromising blood coagulation Platelets play pivotal roles in thromboembolic diseases, such as myocardial infarction and ischemic stroke. In patients envenomed by the snake Vipera a. ammodytes (Vaa), pronounced and transient thrombocytopenia without bleeding is observed. We previously showed that Vaa-snaclec-3/2, the snake venom C-type lectin-like protein, mediates this effect ex vivo. Here, we extended our study of the antithrombotic potential of this protein in vivo using a mouse model of ferric chloride (FeCl3)-induced carotid artery thrombosis. Prior to inducing thrombus formation, the mice received 1, 5, 10, 20, or 50 μg/kg Vaa-snaclec-3/2 intravenously. Afterward, the arterial blood flow was monitored with a perivascular Doppler probe. Additionally, the platelet count in the peripheral venous blood; tail bleeding time; and liver, lung, kidney, spleen, and heart histology were evaluated. The lowest dose of Vaa-snaclec-3/2 that we showed to cause severe thrombocytopenia and completely inhibit FeCl3-induced thrombus formation was 20 µg/kg. This dose prolonged the median tail bleeding time from 86.5 to 153.5 s but did not induce acute spontaneous hemorrhage, as demonstrated by histological analysis. Histology revealed no signs of apoptosis, necrosis or other degenerative changes in the inspected organs of mice exposed to 20 μg/kg Vaa-snaclec-3/2. Platelet clusters were observed only in the lungs, which appear to be the primary site of platelet sequestration and the cause of thrombocytopenia. Taken together, our findings highlight the high potential of Vaa-snaclec-3/2 as a safe and effective antithrombotic agent for the transient prevention of thrombosis in acute clinical settings. snake venom antithrombotic snaclec arterial thrombosis mice kačji strup kače miši true false true Angleški jezik Slovenski jezik Neznano 2026-04-20 12:35:56 2026-04-20 12:35:56 2026-04-21 04:15:24 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 24. 10. 2025; str. 1-14 issue 11, [article no.] 523 Vol. 17 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615:616-092:636.09 2072-6651 10.3390/toxins17110523 254604035 RAZ_Zuzek_Monika_C._2025.pdf RAZ_Zuzek_Monika_C._2025.pdf 1 871AC60620B902304C4084D2D90D5F8D 07248d924467f73ce414404dfbc4970eb4b4722db007c34bda365c68d12142a4 d4ff27e4-3ca4-11f1-92e1-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43418 https://www.mdpi.com/2072-6651/17/11/523 1 ba2930c1-3ca4-11f1-92e1-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=43417 Univerzitetni klinični center Ljubljana 0 0 0