| Title: | Calprest ELISA vs. Liaison® chemiluminescence : evaluating accuracy, efficiency, and clinical utility in fecal calprotectin testing |
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| Authors: | ID Osredkar, Joško (Author)
ID Ekart, Nina (Author)
ID Drobne, David (Author) |
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| Files: |  PDF - Presentation file, download (949,23 KB)
MD5: C7D8A625DC49362780CCB9D3C180F2D9
 URL - Source URL, visit https://www.mdpi.com/2227-9059/14/1/143
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| Language: | English |
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| Typology: | 1.03 - Other scientific articles |
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| Organization: |  UKC LJ - Ljubljana University Medical Centre
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| Abstract: | Background: Ulcerative colitis (UC) management relies on accurately assessing disease activity. Fecal calprotectin (FC) is a promising non-invasive biomarker, but method-specific differences in measurement can affect interpretation. Objective: To compare the performance of Calprest ELISA and DiaSorin Liaison CLIA in measuring FC concentrations and their correlation with endoscopic findings in UC. Methods: Stool samples from 40 UC patients were analyzed using both methods, with 138 samples collected across three clinical timepoints. Spearman’s correlation, Wilcoxon test, Bland–Altman analysis, and ROC curves were used to evaluate method agreement and diagnostic performance relative to Mayo endoscopic scores. Results: A total of 135 paired results showed strong correlation (ρ = 0.795, p < 0.001) but significant inter-method differences (p = 0.039). Liaison tended to yield higher FC values. ROC analysis established optimal cut-offs for detecting endoscopic remission and active disease: 47.95/69.55 µg/g (Liaison) and 65/125 µg/g (Calprest). Calprest demonstrated slightly better diagnostic accuracy. Conclusions: Both methods are reliable for monitoring UC activity. Calprest offers greater dynamic range, while Liaison excels in automation and speed. Method-specific thresholds should guide clinical interpretation to ensure accurate disease monitoring. |
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| Keywords: | ulcerative colitis, fecal calprotectin, statistical analysis, endoscopy, ELISA, CLIA, endoscopic Mayo index |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2026 |
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| Number of pages: | str. [1]-11 |
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| Numbering: | Vol. 11, issue 1, [article no.] 143 |
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| PID: | 20.500.12556/DiRROS-28829  |
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| UDC: | 616.348-002 |
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| ISSN on article: | 2227-9059 |
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| DOI: | 10.3390/biomedicines14010143 |
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| COBISS.SI-ID: | 264601091 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 14. 1. 2026;
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| Publication date in DiRROS: | 08.04.2026 |
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| Views: | 39 |
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| Downloads: | 16 |
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