20.500.12556/DiRROS-28829 Calprest ELISA vs. Liaison® chemiluminescence evaluating accuracy, efficiency, and clinical utility in fecal calprotectin testing Background: Ulcerative colitis (UC) management relies on accurately assessing disease activity. Fecal calprotectin (FC) is a promising non-invasive biomarker, but method-specific differences in measurement can affect interpretation. Objective: To compare the performance of Calprest ELISA and DiaSorin Liaison CLIA in measuring FC concentrations and their correlation with endoscopic findings in UC. Methods: Stool samples from 40 UC patients were analyzed using both methods, with 138 samples collected across three clinical timepoints. Spearman’s correlation, Wilcoxon test, Bland–Altman analysis, and ROC curves were used to evaluate method agreement and diagnostic performance relative to Mayo endoscopic scores. Results: A total of 135 paired results showed strong correlation (ρ = 0.795, p < 0.001) but significant inter-method differences (p = 0.039). Liaison tended to yield higher FC values. ROC analysis established optimal cut-offs for detecting endoscopic remission and active disease: 47.95/69.55 µg/g (Liaison) and 65/125 µg/g (Calprest). Calprest demonstrated slightly better diagnostic accuracy. Conclusions: Both methods are reliable for monitoring UC activity. Calprest offers greater dynamic range, while Liaison excels in automation and speed. Method-specific thresholds should guide clinical interpretation to ensure accurate disease monitoring. ulcerative colitis fecal calprotectin statistical analysis endoscopy ELISA CLIA endoscopic Mayo index ulcerozni kolitis fekalni kalprotektin statistična analiza endoskopija ELISA CLIA endoskopski Mayo indeks true false true Angleški jezik Slovenski jezik Neznano 2026-04-08 14:47:17 2026-04-08 14:47:18 2026-04-09 03:51:24 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Opis vira z dne 14. 1. 2026; str. [1]-11 issue 1, [article no.] 143 Vol. 11 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.348-002 2227-9059 10.3390/biomedicines14010143 264601091 RAZ_Osredkar_Josko_2026.pdf RAZ_Osredkar_Josko_2026.pdf 1 C7D8A625DC49362780CCB9D3C180F2D9 4764392bb99f4dae0b77e135e302f5f0067eb188de89af0e59930ef7dc26c5da 3dbc8c7d-3349-11f1-b941-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42958 https://www.mdpi.com/2227-9059/14/1/143 1 15538abd-3349-11f1-b941-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=42957 Univerzitetni klinični center Ljubljana 0 0 0