| Title: | Critical evaluation of enzymatic extraction for quantification of europium-doped polystyrene nanoplastics in tomato tissues by single particle ICP-MS |
|---|
| Authors: | ID Sahai, Harshit (Author)
ID Leban, Pia, Institut "Jožef Stefan" (Author)
ID Heath, Ester, Institut "Jožef Stefan" (Author)
ID Kacjan-Maršić, Nina (Author)
ID Železnikar, Špela (Author)
ID Podlogar, Matejka, Institut "Jožef Stefan" (Author)
ID Radošević, Tina, Institut "Jožef Stefan" (Author)
ID Martinez Bueno, María Jesús (Author)
ID Vidmar, Janja, Institut "Jožef Stefan" (Author) |
|---|
| Files: |  URL - Source URL, visit https://www.sciencedirect.com/science/article/pii/S0147651326002022?via%3Dihub
 PDF - Presentation file, download (6,56 MB)
MD5: 2C99E9F5774093AC33EADD56E4809EA6
|
|---|
| Language: | English |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: |  IJS - Jožef Stefan Institute
|
|---|
| Abstract: | This study critically evaluates an analytical approach combining enzymatic extraction and single particle ICP-MS (spICP-MS) for quantifying europium-doped polystyrene nanoplastics (Eu-PS-NPs) bioaccumulated in tomato tissues. Optimization of extraction parameters identified citrate buffer at pH 6.5 and a digestion temperature of 37 °C as the most effective extraction conditions, while maintaining particle stability. Experiments with spiked tomato tissues demonstrated that extraction efficiency is highly tissue-specific, with optimal digestion of 24 h for stem, leaf, and fruit, and 36 h for root tissues, and enzyme concentrations ranging from 10 to 100 mg per sample. Under optimized conditions, good extraction recoveries (85 – 116 %) were achieved for particle number and mass concentrations, particle size and mass, and total Eu mass, with the majority of extracted Eu associated with NPs and around 10 % as ionic Eu. In contrast, analysis of tomato samples exposed to Eu-PS-NPs during their growth revealed substantially lower and tissue-dependent extraction recoveries. Root and stem tissues yielded only 18 – 32 % of total Eu mass concentration, while leaves showed recoveries ≤ 21 % under most extraction conditions. Fruit samples exhibited higher apparent recoveries (66 – 80 % after 24 h digestion), likely due to the acidic environment promoting Eu leaching from NPs. Across all exposed tissues, ionic Eu fraction dominates (reaching up to 97 % in fruits), indicating extensive leaching from Eu-PS-NPs in the tomato plants. These findings underscore the importance of accounting for matrix effects, metal leaching, and the limitation of extrapolating recoveries from spiked controls to exposed samples when interpreting spICP-MS data from plant exposure studies with metal-doped NPs. |
|---|
| Keywords: | nanoplastics, europium |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Submitted for review: | 01.10.2025 |
|---|
| Article acceptance date: | 09.02.2026 |
|---|
| Publication date: | 11.02.2026 |
|---|
| Publisher: | Elsevier |
|---|
| Year of publishing: | 2026 |
|---|
| Number of pages: | str. 1-13 |
|---|
| Numbering: | Vol. 311, [article no.] 119873 |
|---|
| Source: | Nizozemska |
|---|
| PID: | 20.500.12556/DiRROS-27667  |
|---|
| UDC: | 54 |
|---|
| ISSN on article: | 1090-2414 |
|---|
| DOI: | 10.1016/j.ecoenv.2026.119873 |
|---|
| COBISS.SI-ID: | 268580611 |
|---|
| Copyright: | © 2026 The Authors. |
|---|
| Note: | Nasl. z nasl. zaslona;
Soavtorice iz Slovenije: Pia Leban, Ester Heath, Nina Kacjan Maršić, Špela Železnikar, Matejka Podlogar, Tina Radošević, Janja Vidmar;
Opis vira z dne 16. 2. 2026;
|
|---|
| Publication date in DiRROS: | 18.02.2026 |
|---|
| Views: | 41 |
|---|
| Downloads: | 19 |
|---|
| Metadata: |    |
|---|
|
:
|
Copy citation |
|---|
| | | | Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
