| Title: | Reduced ATP turnover during hibernation in relaxed skeletal muscle |
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| Authors: | ID De Napoli, Cosimo (Author)
ID Schmidt, Luisa (Author)
ID Montesel, Mauro (Author)
ID Cussonneau, Laura (Author)
ID Sanniti, Samuele (Author)
ID Marcucci, Lorenzo (Author)
ID Germinario, Elena (Author)
ID Kindberg, Jonas (Author)
ID Evans, Alina L. (Author)
ID Narici, Marco Vincenzo (Author) |
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| Files: |  PDF - Presentation file, download (2,43 MB)
MD5: 154F23FC648FF3F313F100F3D13C255B
 URL - Source URL, visit https://www.nature.com/articles/s41467-024-55565-4
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  ZRS Koper - Science and Research Centre Koper
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| Abstract: | Hibernating brown bears, due to a drastic reduction in metabolic rate, show only moderate muscle wasting. Here, we evaluate if ATPase activity of resting skeletal muscle myosin can contribute to this energy sparing. By analyzing single muscle fibers taken from the samebears,eitherduring hibernationorin summer,wefindthatfibersfromhibernatingbearshaveamilddeclineinforce production and a significant reduction in ATPase activity. Single fiber proteomics, western blotting, and immunohistochemical analyses reveal major remodeling of the mitochondrial proteome during hibernation. Furthermore, using bioinformatical approaches and western blotting we find that phosphorylated myosin light chain, a known stimulator of basal myosin ATPase activity, is decreased in hibernating and disused muscles. These results suggestthatskeletalmusclelimitsenergylossbyreducingmyosinATPaseactivity, indicating a possible role for myosin ATPase activity modulation in multiple muscle wasting conditions. |
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| Keywords: | translational research, animal physiology, metabolism |
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| Publication version: | Version of Record |
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| Publication date: | 01.01.2025 |
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| Year of publishing: | 2025 |
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| Number of pages: | str. 1-14 |
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| Numbering: | Vol. 16, art. no. ǂ80 |
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| PID: | 20.500.12556/DiRROS-25252  |
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| UDC: | 591.5 |
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| ISSN on article: | 2041-1723 |
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| DOI: | 10.1038/s41467-024-55565-4 |
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| COBISS.SI-ID: | 264672515 |
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| Copyright: | ©The Author(s) |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 14. 1. 2026;
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| Publication date in DiRROS: | 14.01.2026 |
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| Views: | 58 |
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| Downloads: | 29 |
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| Metadata: |    |
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