20.500.12556/DiRROS-25252 Reduced ATP turnover during hibernation in relaxed skeletal muscle Hibernating brown bears, due to a drastic reduction in metabolic rate, show only moderate muscle wasting. Here, we evaluate if ATPase activity of resting skeletal muscle myosin can contribute to this energy sparing. By analyzing single muscle fibers taken from the samebears,eitherduring hibernationorin summer,wefindthatfibersfromhibernatingbearshaveamilddeclineinforce production and a significant reduction in ATPase activity. Single fiber proteomics, western blotting, and immunohistochemical analyses reveal major remodeling of the mitochondrial proteome during hibernation. Furthermore, using bioinformatical approaches and western blotting we find that phosphorylated myosin light chain, a known stimulator of basal myosin ATPase activity, is decreased in hibernating and disused muscles. These results suggestthatskeletalmusclelimitsenergylossbyreducingmyosinATPaseactivity, indicating a possible role for myosin ATPase activity modulation in multiple muscle wasting conditions. translational research animal physiology metabolism translacijsko raziskovanje živalska fiziologija presnova true false false Angleški jezik Slovenski jezik ©The Author(s) Neznano 2026-01-14 12:23:17 2026-01-14 12:23:17 2026-01-15 03:48:55 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 14. 1. 2026; str. 1-14 art. no. ǂ80 Vol. 16 Jan. 2025 0000-00-00 Zaloznikova NiDoloceno NiDoloceno 0000-00-00 0000-00-00 2025-01-01 591.5 2041-1723 10.1038/s41467-024-55565-4 264672515 26-01-14-Narici-264672515.pdf 26-01-14-Narici-264672515.pdf 1 154F23FC648FF3F313F100F3D13C255B 5f0b904434f49577d196aa25fd55eb3bad49198ecd5b67c18eaa9217ba480a57 8687dee6-f13b-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=38122 https://www.nature.com/articles/s41467-024-55565-4 1 6f6d5732-f13b-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=38121 Znanstveno-raziskovalno središče Koper / Centro di Ricerche Scientifiche Capodistria 0 0 0