| Title: | Protein stacking on the APTES-functionalized pyrochlore ▫$Bi_2Ru_2O_7$▫ clusters for ultrasensitive and selective immunosensing |
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| Authors: | ID Tasić, Nikola (Author)
ID Vranešič, Nika (Author)
ID Metarapi, Dino (Author)
ID Mervič, Kristina (Author)
ID Žunić, Milan (Author)
ID Dapčević, Aleksandra (Author)
ID Finšgar, Matjaž (Author)
ID Hočevar, Samo B. (Author) |
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| Files: |  URL - Source URL, visit https://pubs.acs.org/doi/10.1021/acsami.4c17869
 PDF - Presentation file, download (1,47 MB)
MD5: 6ED82F843731F978E824727C0F952C75
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  KI - National Institute of Chemistry
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| Abstract: | With their unique physicochemical properties, such as metallic-like conductivity, favorable (electro)catalytic properties, electrochemical stability, and ease of functionalization, pyrochlores have found applications in various fields such as solid oxide fuel cells, batteries, thick film resistors, and temperature sensors; however, there are no reports on their application in electrochemical immunosensing. In this study, we exploited the (electro)catalytic nature and stability of the pyrochlore Bi2Ru2O7 clusters silanized with (3-aminopropyl)triethoxysilane (APTES) to demonstrate their potential for the effective stacking of functional proteins. Characterization of the clusters by XPS disclosed a dual environment of Bi, also indicating the presence of Bi2O3 alongside APTES-Bi2Ru2O7 clusters and, importantly, the predominant involvement of pyrochlore moieties in subsequent protein stacking. After stacking protein A and antibodies, the immunosensor revealed a nearly interference-free operation, high sensitivity, a detection limit of 118 fM SARS-CoV-2 spike protein, and operation in a wide examined concentration range of 10−5−10−1 μg mL−1 with an r2 of 0.98. In combination with a short incubation time of 30 min, the pyrochlore-based immunosensor provides a solid platform for future point-of-need applications. |
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| Keywords: | Bi2Ru2O7 pyrochlore, APTES, SARS-CoV-2, spike protein, immunosensors, electrochemical |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.01.2025 |
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| Publisher: | American Chemical Society |
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| Year of publishing: | 2025 |
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| Number of pages: | str. 10792-10801 |
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| Numbering: | Vol. 17, iss. 7 |
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| Source: | ACS applied materials & interfaces |
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| PID: | 20.500.12556/DiRROS-21753  |
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| UDC: | 543.384 |
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| ISSN on article: | 1944-8252 |
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| DOI: | 10.1021/acsami.4c17869 |
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| COBISS.SI-ID: | 228608771 |
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| Copyright: | © 2025 The Authors. Published by American Chemical Society |
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| Note: | Nasl. z nasl. zaslona;
Soavtorji: Nika Vranešič, Dino Metarapi, Kristina Mervič, Milan Žunić, Aleksandra Dapčević, Matjaž Finšgar, Samo B. Hočevar;
Opis vira z dne 11. 3. 2025;
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| Publication date in DiRROS: | 24.03.2025 |
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| Views: | 672 |
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| Downloads: | 403 |
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