20.500.12556/DiRROS-21753 Protein stacking on the APTES-functionalized pyrochlore ▫$Bi_2Ru_2O_7$▫ clusters for ultrasensitive and selective immunosensing With their unique physicochemical properties, such as metallic-like conductivity, favorable (electro)catalytic properties, electrochemical stability, and ease of functionalization, pyrochlores have found applications in various fields such as solid oxide fuel cells, batteries, thick film resistors, and temperature sensors; however, there are no reports on their application in electrochemical immunosensing. In this study, we exploited the (electro)catalytic nature and stability of the pyrochlore Bi2Ru2O7 clusters silanized with (3-aminopropyl)triethoxysilane (APTES) to demonstrate their potential for the effective stacking of functional proteins. Characterization of the clusters by XPS disclosed a dual environment of Bi, also indicating the presence of Bi2O3 alongside APTES-Bi2Ru2O7 clusters and, importantly, the predominant involvement of pyrochlore moieties in subsequent protein stacking. After stacking protein A and antibodies, the immunosensor revealed a nearly interference-free operation, high sensitivity, a detection limit of 118 fM SARS-CoV-2 spike protein, and operation in a wide examined concentration range of 10−5−10−1 μg mL−1 with an r2 of 0.98. In combination with a short incubation time of 30 min, the pyrochlore-based immunosensor provides a solid platform for future point-of-need applications. Bi2Ru2O7 pyrochlore APTES SARS-CoV-2 spike protein immunosensors electrochemical elektrokemija piroklor proteini beljakovine kataliza true false true American Chemical Society ACS applied materials & interfaces Angleški jezik Slovenski jezik © 2025 The Authors. Published by American Chemical Society Neznano 2025-03-24 09:26:18 2025-03-24 09:39:05 2025-10-24 03:49:45 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Soavtorji: Nika Vranešič, Dino Metarapi, Kristina Mervič, Milan Žunić, Aleksandra Dapčević, Matjaž Finšgar, Samo B. Hočevar; Opis vira z dne 11. 3. 2025; str. 10792-10801 iss. 7 Vol. 17 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-01-01 543.384 1944-8252 10.1021/acsami.4c17869 228608771 https://pubs.acs.org/doi/10.1021/acsami.4c17869 1 aa056c5e-0889-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31324 RAZ_Tasic_Nikola_2025.pdf RAZ_Tasic_Nikola_2025.pdf 1 6ED82F843731F978E824727C0F952C75 cb5c534ec0a1cd09edd103aa5b698b2a41fdcd818f0519a5a7d83f025551dbbc 273abb48-088b-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31325 Kemijski inštitut 0 0 0