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Title:Mitochondria can substitute for parvalbumin to lowercytosolic calcium levels in the murine fast skeletal muscle
Authors:ID Marcucci, Lorenzo (Author)
ID Nogara, Leonardo (Author)
ID Canato, Marta (Author)
ID Germinario, Elena (Author)
ID Raffaello, Anna (Author)
ID Carraro, Michela (Author)
ID Bernardi, Paolo (Author)
ID Pietrangelo, Laura (Author)
ID Boncompagni, Simona (Author)
ID Protasi, Feliciano (Author)
ID Paolocci, Nazareno (Author)
ID Reggiani, Carlo (Author)
Files:.pdf PDF - Presentation file, download (3,97 MB)
MD5: 444CDA853D07DE6199EF14F51714D3ED
 
URL URL - Source URL, visit https://onlinelibrary.wiley.com/doi/epdf/10.1111/apha.14208
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo ZRS Koper - Science and Research Centre Koper
Abstract:Aim: Parvalbumin (PV) is a primary calcium buffer in mouse fast skeletal musclefibers. Previous work showed that PV ablation has a limited impact on cytosolicCa2+ ([Ca2+]cyto) transients and contractile response, while it enhances mitochon-drial density and mitochondrial matrix-free calcium concentration ([Ca2+]mito).Here, we aimed to quantitatively test the hypothesis that mitochondria act tocompensate for PV deficiency.Methods: We determined the free Ca 2+ redistribution during a 2 s 60 Hz tetanicstimulation in the sarcoplasmic reticulum, cytosol, and mitochondria. Via a re-action–diffusion Ca 2+ model, we quantitatively evaluated mitochondrial uptakeand storage capacity requirements to compensate for PV lack and analyzed pos-sible extracellular export.Results: [Ca 2+]mito during tetanic stimulation is greater in knock-out (KO)(1362 ± 392 nM) than in wild-type (WT) (855 ± 392 nM), p < 0.05. Under the as-sumption of a non-linear intramitochondrial buffering, the model predicts an ac-cumulation of 725 μmoles/Lfiber (buffering ratio 1:11 000) in KO, much higherthan in WT (137 μmoles/Lfiber, ratio 1:4500). The required transport rate via mi-tochondrial calcium uniporter (MCU) reaches 3 mM/s, compatible with availableliterature. TEM images of calcium entry units and Mn2+ quenching showed a greater capacity of store- operated calcium entry in KO compared to WT. However,levels of [Ca 2+]cyto during tetanic stimulation were not modulated to variations ofextracellular calcium.Conclusions: The model-based analysis of experimentally determined calciumdistribution during tetanic stimulation showed that mitochondria can act as abuffer to compensate for the lack of PV. This result contributes to a better under-standing of mitochondria's role in modulating [Ca2+]cyto in skeletal muscle fibers.
Keywords:calcium, mitochondria, mouse skeletal muscle fibers, parvalbumin, reaction-diffusion model
Publication version:Version of Record
Submitted for review:09.07.2024
Article acceptance date:15.07.2024
Publication date:30.07.2024
Year of publishing:2024
Number of pages:str. 1-14
Numbering:Vol. 240, ǂiss.ǂ9
PID:20.500.12556/DiRROS-20828 New window
UDC:612
ISSN on article:1748-1716
DOI:10.1111/apha.14208 New window
COBISS.SI-ID:214814467 New window
Copyright:© 2024 The Author(s)
Note:Opis vira z dne 13. 11. 2024; Nasl. z nasl. zaslona;
Publication date in DiRROS:13.11.2024
Views:279
Downloads:2972
Metadata:XML DC-XML DC-RDF
:
MARCUCCI, Lorenzo, NOGARA, Leonardo, CANATO, Marta, GERMINARIO, Elena, RAFFAELLO, Anna, CARRARO, Michela, BERNARDI, Paolo, PIETRANGELO, Laura, BONCOMPAGNI, Simona, PROTASI, Feliciano, PAOLOCCI, Nazareno and REGGIANI, Carlo, 2024, Mitochondria can substitute for parvalbumin to lowercytosolic calcium levels in the murine fast skeletal muscle. Acta physiologica [online]. 2024. Vol. 240, no. 9, p. 1–14. [Accessed 6 April 2025]. DOI 10.1111/apha.14208. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=20828
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Record is a part of a journal

Title:Acta physiologica
Publisher:Blackwell Publishing Ltd
ISSN:1748-1716
COBISS.SI-ID:2917396 New window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:30.07.2024

Secondary language

Language:Slovenian
Keywords:kalcij, mitohondriji, mišična vlakna skeletnih mišic miši, parvalbumin, reaction–diffusion model


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