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Naslov:Mitochondria can substitute for parvalbumin to lowercytosolic calcium levels in the murine fast skeletal muscle
Avtorji:ID Marcucci, Lorenzo (Avtor)
ID Nogara, Leonardo (Avtor)
ID Canato, Marta (Avtor)
ID Germinario, Elena (Avtor)
ID Raffaello, Anna (Avtor)
ID Carraro, Michela (Avtor)
ID Bernardi, Paolo (Avtor)
ID Pietrangelo, Laura (Avtor)
ID Boncompagni, Simona (Avtor)
ID Protasi, Feliciano (Avtor)
ID Paolocci, Nazareno (Avtor)
ID Reggiani, Carlo (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (3,97 MB)
MD5: 444CDA853D07DE6199EF14F51714D3ED
 
URL URL - Izvorni URL, za dostop obiščite https://onlinelibrary.wiley.com/doi/epdf/10.1111/apha.14208
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo ZRS Koper - Znanstveno-raziskovalno središče Koper / Centro di Ricerche Scientifiche Capodistria
Povzetek:Aim: Parvalbumin (PV) is a primary calcium buffer in mouse fast skeletal musclefibers. Previous work showed that PV ablation has a limited impact on cytosolicCa2+ ([Ca2+]cyto) transients and contractile response, while it enhances mitochon-drial density and mitochondrial matrix-free calcium concentration ([Ca2+]mito).Here, we aimed to quantitatively test the hypothesis that mitochondria act tocompensate for PV deficiency.Methods: We determined the free Ca 2+ redistribution during a 2 s 60 Hz tetanicstimulation in the sarcoplasmic reticulum, cytosol, and mitochondria. Via a re-action–diffusion Ca 2+ model, we quantitatively evaluated mitochondrial uptakeand storage capacity requirements to compensate for PV lack and analyzed pos-sible extracellular export.Results: [Ca 2+]mito during tetanic stimulation is greater in knock-out (KO)(1362 ± 392 nM) than in wild-type (WT) (855 ± 392 nM), p < 0.05. Under the as-sumption of a non-linear intramitochondrial buffering, the model predicts an ac-cumulation of 725 μmoles/Lfiber (buffering ratio 1:11 000) in KO, much higherthan in WT (137 μmoles/Lfiber, ratio 1:4500). The required transport rate via mi-tochondrial calcium uniporter (MCU) reaches 3 mM/s, compatible with availableliterature. TEM images of calcium entry units and Mn2+ quenching showed a greater capacity of store- operated calcium entry in KO compared to WT. However,levels of [Ca 2+]cyto during tetanic stimulation were not modulated to variations ofextracellular calcium.Conclusions: The model-based analysis of experimentally determined calciumdistribution during tetanic stimulation showed that mitochondria can act as abuffer to compensate for the lack of PV. This result contributes to a better under-standing of mitochondria's role in modulating [Ca2+]cyto in skeletal muscle fibers.
Ključne besede:calcium, mitochondria, mouse skeletal muscle fibers, parvalbumin, reaction-diffusion model
Verzija publikacije:Objavljena publikacija
Poslano v recenzijo:09.07.2024
Datum sprejetja članka:15.07.2024
Datum objave:30.07.2024
Leto izida:2024
Št. strani:str. 1-14
Številčenje:Vol. 240, ǂiss.ǂ9
PID:20.500.12556/DiRROS-20828 Novo okno
UDK:612
ISSN pri članku:1748-1716
DOI:10.1111/apha.14208 Novo okno
COBISS.SI-ID:214814467 Novo okno
Avtorske pravice:© 2024 The Author(s)
Opomba:Opis vira z dne 13. 11. 2024; Nasl. z nasl. zaslona;
Datum objave v DiRROS:13.11.2024
Število ogledov:286
Število prenosov:2975
Metapodatki:XML DC-XML DC-RDF
:
MARCUCCI, Lorenzo, NOGARA, Leonardo, CANATO, Marta, GERMINARIO, Elena, RAFFAELLO, Anna, CARRARO, Michela, BERNARDI, Paolo, PIETRANGELO, Laura, BONCOMPAGNI, Simona, PROTASI, Feliciano, PAOLOCCI, Nazareno in REGGIANI, Carlo, 2024, Mitochondria can substitute for parvalbumin to lowercytosolic calcium levels in the murine fast skeletal muscle. Acta physiologica [na spletu]. 2024. Vol. 240, no. 9, p. 1–14. [Dostopano 11 april 2025]. DOI 10.1111/apha.14208. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=20828
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Gradivo je del revije

Naslov:Acta physiologica
Založnik:Blackwell Publishing Ltd
ISSN:1748-1716
COBISS.SI-ID:2917396 Novo okno

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:30.07.2024

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:kalcij, mitohondriji, mišična vlakna skeletnih mišic miši, parvalbumin, reaction–diffusion model


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