| Title: | A primer on the analysis of high-throughput sequencing data for detection of plant viruses |
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| Authors: | ID Kutnjak, Denis (Author)
ID Tamisier, Lucie (Author)
ID Adams, Ian (Author)
ID Boonham, Neil (Author)
ID Candresse, Thierry (Author)
ID Chiumenti, Michela (Author)
ID De Jonghe, Kris (Author)
ID Kreuze, Jan F. (Author)
ID Lefebvre, Marie (Author)
ID Silva, Goncalo (Author)
ID Malapi-Wight, Martha (Author)
ID Margaria, Paolo (Author)
ID Mavrič Pleško, Irena (Author)
ID McGreig, Sam (Author)
ID Miozzi, Laura (Author)
ID Remenant, Benoit (Author)
ID Reynard, Jean-Sébastien (Author)
ID Rollin, Johan (Author)
ID Rott, Mike (Author)
ID Schumpp, Olivier (Author)
ID Massart, Sébastien (Author)
ID Haegeman, Annelies (Author) |
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| Files: |  URL - Source URL, visit https://www.mdpi.com/2076-2607/9/4/841
 PDF - Presentation file, download (3,87 MB)
MD5: C8EF082B94A6B563D8932C4C809E66A4
URL - Source URL, visit https://doi.org/10.3390/microorganisms9040841
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| Language: | English |
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| Typology: | 1.02 - Review Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | High-throughput sequencing (HTS) technologies have become indispensable tools assisting plant virus diagnostics and research thanks to their ability to detect any plant virus in a sample without prior knowledge. As HTS technologies are heavily relying on bioinformatics analysis of the huge amount of generated sequences, it is of utmost importance that researchers can rely on efficient and reliable bioinformatic tools and can understand the principles, advantages, and disadvantages of the tools used. Here, we present a critical overview of the steps involved in HTS as employed for plant virus detection and virome characterization. We start from sample preparation and nucleic acid extraction as appropriate to the chosen HTS strategy, which is followed by basic data analysis requirements, an extensive overview of the in-depth data processing options, and taxonomic classification of viral sequences detected. By presenting the bioinformatic tools and a detailed overview of the consecutive steps that can be used to implement a well-structured HTS data analysis in an easy and accessible way, this paper is targeted at both beginners and expert scientists engaging in HTS plant virome projects. |
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| Keywords: | plant virus, high-throughput sequencing, bioinformatics, detection, discovery |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 14.04.2021 |
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| Year of publishing: | 2021 |
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| Number of pages: | str. 1-31 |
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| Numbering: | Vol. 9, iss. 4 |
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| PID: | 20.500.12556/DiRROS-20110  |
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| UDC: | 632 |
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| ISSN on article: | 2076-2607 |
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| DOI: | 10.3390/microorganisms9040841 |
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| COBISS.SI-ID: | 59812099 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 15. 4. 2021;
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| Publication date in DiRROS: | 05.08.2024 |
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| Views: | 310 |
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| Downloads: | 259 |
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