20.500.12556/DiRROS-20110 A primer on the analysis of high-throughput sequencing data for detection of plant viruses High-throughput sequencing (HTS) technologies have become indispensable tools assisting plant virus diagnostics and research thanks to their ability to detect any plant virus in a sample without prior knowledge. As HTS technologies are heavily relying on bioinformatics analysis of the huge amount of generated sequences, it is of utmost importance that researchers can rely on efficient and reliable bioinformatic tools and can understand the principles, advantages, and disadvantages of the tools used. Here, we present a critical overview of the steps involved in HTS as employed for plant virus detection and virome characterization. We start from sample preparation and nucleic acid extraction as appropriate to the chosen HTS strategy, which is followed by basic data analysis requirements, an extensive overview of the in-depth data processing options, and taxonomic classification of viral sequences detected. By presenting the bioinformatic tools and a detailed overview of the consecutive steps that can be used to implement a well-structured HTS data analysis in an easy and accessible way, this paper is targeted at both beginners and expert scientists engaging in HTS plant virome projects. plant virus high-throughput sequencing bioinformatics detection discovery varstvo rastlin rastlinski virus bioinformatika mikroorganizmi zaznavanje odkritje true false true Angleški jezik Slovenski jezik Neznano 2024-08-05 16:13:26 2024-08-05 16:13:27 2024-08-07 04:01:50 0000-00-00 00:00:00 2021 0 0 Nasl. z nasl. zaslona; Opis vira z dne 15. 4. 2021; str. 1-31 iss. 4 Vol. 9 14 Apr. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-04-14 632 2076-2607 10.3390/microorganisms9040841 59812099 https://www.mdpi.com/2076-2607/9/4/841 1 21127df0-5324-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28042 RAZ_Kutnjak_Denis_2021.pdf RAZ_Kutnjak_Denis_2021.pdf 1 C8EF082B94A6B563D8932C4C809E66A4 eb186a6f67b2c1f0ecdd9af31520bb800418fdbc77dcdfaacb40b125525a4a24 b6f14eeb-5324-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28043 https://doi.org/10.3390/microorganisms9040841 0 c8638623-5324-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28044 Nacionalni inštitut za biologijo 0 0 0