| Title: | CRISPR/Cas-mediated plant genome editing : outstanding challenges a decade after implementation |
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| Authors: | ID Cardi, Teodoro (Author)
ID Murovec, Jana (Author)
ID Bakhsh, Allah (Author)
ID Galović, Vladislava (Author)
ID Lukan, Tjaša (Author)
ID Yıldırım, Kubilay (Author)
ID Zlatković, Milica (Author)
ID Van Laere, Katrijn (Author) |
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| Files: |  URL - Source URL, visit https://www.cell.com/action/showPdf?pii=S1360-1385%2823%2900164-4
 PDF - Presentation file, download (4,40 MB)
MD5: 8F76B958351D20986DDA9CD15E8DF917
URL - Source URL, visit https://doi.org/10.1016/j.tplants.2023.05.012
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| Language: | English |
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| Typology: | 1.02 - Review Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding of the plant genome. CRISPR/Cas has been used for over a decade to modify plant genomes for the study of specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops. Although the CRISPR/Cas system is very efficient for genome editing, many bottlenecks and challenges slow down further improvement and applications. In this review we discuss the challenges that can occur during tissue culture, transformation, regeneration, and mutant detection. We also review the opportunities provided by new CRISPR platforms and specific applications related to gene regulation, abiotic and biotic stress response improvement, and de novo domestication of plants. |
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| Keywords: | CRISPR applications, CRISPR platforms, gene regulations, mutant detection, plant regeneration |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.10.2023 |
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| Year of publishing: | 2023 |
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| Number of pages: | Str. 1144-1165 |
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| Numbering: | Vol. 28, iss. 10 |
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| PID: | 20.500.12556/DiRROS-20063  |
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| UDC: | 60 |
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| ISSN on article: | 1360-1385 |
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| DOI: | 10.1016/j.tplants.2023.05.012 |
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| COBISS.SI-ID: | 156416259 |
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| Publication date in DiRROS: | 05.08.2024 |
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| Views: | 265 |
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| Downloads: | 260 |
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| Metadata: |    |
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