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Title:Survey results on nucleic acid tests of infectious diseases : present status and need for rapid and near-patient diagnostics
Authors:ID Neukammer, Jörg (Author)
ID Hussels, Martin (Author)
ID Kummrow, Andreas (Author)
ID Devonshire, Alison S. (Author)
ID Foy, Carole A. (Author)
ID Huggett, Jim F. (Author)
ID Parkes, Helen C. (Author)
ID Žel, Jana (Author)
ID Milavec, Mojca (Author)
ID Schimmel, Heinz (Author)
ID Unger, Wolfgang (Author)
ID Akgöz, Müslüm (Author)
ID McHugh, Timothy D. (Author)
ID Tomič, Viktorija (Author)
ID Grunert, Hans-Peter (Author)
ID Zeichhardt, Heinz (Author)
Files:URL URL - Source URL, visit http://www.egms.de/static/pdf/journals/lab/2015-6/lab000016.pdf
 
.pdf PDF - Presentation file, download (5,32 MB)
MD5: AC9DEA49636CC29DDC897132B4899313
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:This survey discusses current and emerging isothermal and rapid polymerase chain reaction (PCR) based nucleic acid amplification methods for near-patient diagnostics. To assess the clinical need of rapid diagnostics for infectious diseases based on nucleic acid tests (NATs) we performed and analysed a questionnaire among laboratories participating in corresponding INSTAND ring trials for external quality assurance. The questions concerning new amplification technologies like isothermal nucleic acid amplification, potentially suited to significantly decrease turnaround times, were complemented by questions to evaluate the present status of NATs. Besides end-users, companies were also addressed by sending out a manufacturer specific questionnaire. Analysis of the answers from 48 laboratories in 14 European countries revealed that a much shorter turnaround time is requested for selected pathogens compared to about 2 h or longer when applying temperature cycling amplification, i.e. PCR. In this context, most frequently mentioned were methicillin-resistant Staphylococcus aureus (MRSA), norovirus, influenza A and B viruses, cytomegalovirus (CMV) as well as hepatitis B virus (HBV) and hepatitis C virus (HCV). At present, 8% of the laboratories having participated in this survey apply isothermal amplification of nucleic acids to identify infectious pathogens.
Keywords:nucleic acid tests, infectious diseases, virus detection, bacteria detection, isothermal nucleic acid amplification, status report, questionnaire, NAT, PCR
Publication status:Published
Publication version:Version of Record
Publication date:11.02.2015
Year of publishing:2015
Number of pages:str. 1-12
Numbering:Vol. 6
PID:20.500.12556/DiRROS-19785 New window
UDC:61
ISSN on article:1869-4241
DOI:10.3205/lab000016 New window
COBISS.SI-ID:3412815 New window
Note:Nasl. z nasl. zaslona; Opis vira z dne 27. 5. 2015;
Publication date in DiRROS:26.07.2024
Views:454
Downloads:392
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Record is a part of a journal

Title:GMS : Zeitschrift zur förderung der qualitätssicherung in medizinischen laboratorien
Shortened title:GMS, Z. Förd. Qual.sich. med. Lab.
Publisher:INSTAND e.V.
ISSN:1869-4241
COBISS.SI-ID:3412559 New window

Document is financed by a project

Funder:Other - Other funder or multiple funders
Funding programme:European Metrology Research Programme (EMRP)
Project number:HLT-08, 2011
Acronym:INFECT-MET

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

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