20.500.12556/DiRROS-19785 Survey results on nucleic acid tests of infectious diseases present status and need for rapid and near-patient diagnostics This survey discusses current and emerging isothermal and rapid polymerase chain reaction (PCR) based nucleic acid amplification methods for near-patient diagnostics. To assess the clinical need of rapid diagnostics for infectious diseases based on nucleic acid tests (NATs) we performed and analysed a questionnaire among laboratories participating in corresponding INSTAND ring trials for external quality assurance. The questions concerning new amplification technologies like isothermal nucleic acid amplification, potentially suited to significantly decrease turnaround times, were complemented by questions to evaluate the present status of NATs. Besides end-users, companies were also addressed by sending out a manufacturer specific questionnaire. Analysis of the answers from 48 laboratories in 14 European countries revealed that a much shorter turnaround time is requested for selected pathogens compared to about 2 h or longer when applying temperature cycling amplification, i.e. PCR. In this context, most frequently mentioned were methicillin-resistant Staphylococcus aureus (MRSA), norovirus, influenza A and B viruses, cytomegalovirus (CMV) as well as hepatitis B virus (HBV) and hepatitis C virus (HCV). At present, 8% of the laboratories having participated in this survey apply isothermal amplification of nucleic acids to identify infectious pathogens. nucleic acid tests infectious diseases virus detection bacteria detection isothermal nucleic acid amplification status report questionnaire NAT PCR true false true Angleški jezik Ni določen Neznano 2024-07-25 04:13:21 2024-07-26 13:35:39 2024-07-27 04:00:54 0000-00-00 00:00:00 2015 0 0 Nasl. z nasl. zaslona; Opis vira z dne 27. 5. 2015; str. 1-12 Vol. 6 2015 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2015-02-11 61 1869-4241 10.3205/lab000016 3412815 http://www.egms.de/static/pdf/journals/lab/2015-6/lab000016.pdf 1 b4022006-4a1a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27403 RAZ_Neukammer_Jorg_2015.pdf RAZ_Neukammer_Jorg_2015.pdf 1 AC9DEA49636CC29DDC897132B4899313 9a5489a206beec158a773924d0a1a401422be39d53f87c509eba4c06934805a1 af04af04-4b32-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27632 Nacionalni inštitut za biologijo 0 0 0