| Title: | Rapid loop-mediated isothermal amplification assays for grapevine yellows phytoplasmas on crude leaf-vein homogenate has the same performance as qPCR |
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| Authors: | ID Kogovšek, Polona (Author)
ID Mehle, Nataša (Author)
ID Pugelj, Anja (Author)
ID Jakomin, Tjaša (Author)
ID Schroers, Hans-Josef (Author)
ID Ravnikar, Maja (Author)
ID Dermastia, Marina (Author) |
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| Files: |  PDF - Presentation file, download (335,75 KB)
MD5: 11C1F9D71E4A5550EF502263A98754A7
 URL - Source URL, visit https://doi.org/10.1007/s10658-016-1070-z
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
 KIS - Agricultural Institute of Slovenia
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| Abstract: | A fluorescence-based real-time loop-mediated isothermal amplification (LAMP) assay for ‘Candidatus Phytoplasama solani’ (Bois noir phytoplasma; BNp) detection was developed and optimised for rapid laboratory and on-site BNp detection. This assay is highly specific, rapid and as sensitive as qPCR. It was validated according to European and Mediterranean Plant Protection Organisation recommendations. In addition, 286 grapevine leaf samples from the 2015 growing season were tested with this new real-time LAMP assay and an assay previously developed for detection of Flavescence dorée phytoplasma (FDp). These LAMP assays for detection of both BNp and FDp used without any DNA extraction step, which is a required step for qPCR analysis, were comparably effective to qPCR, and positive results were obtained in less than 35 min. |
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| Keywords: | real-time LAMP, grapevine yellows phytoplasma, validation |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.01.2017 |
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| Year of publishing: | 2017 |
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| Number of pages: | str. 75-84 |
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| Numbering: | Vol. 148, iss. 1 |
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| PID: | 20.500.12556/DiRROS-19701  |
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| UDC: | 581.2 |
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| ISSN on article: | 0929-1873 |
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| DOI: | 10.1007/s10658-016-1070-z |
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| COBISS.SI-ID: | 4088399 |
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| Publication date in DiRROS: | 24.07.2024 |
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| Views: | 396 |
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| Downloads: | 230 |
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