20.500.12556/DiRROS-19701 Rapid loop-mediated isothermal amplification assays for grapevine yellows phytoplasmas on crude leaf-vein homogenate has the same performance as qPCR A fluorescence-based real-time loop-mediated isothermal amplification (LAMP) assay for ‘Candidatus Phytoplasama solani’ (Bois noir phytoplasma; BNp) detection was developed and optimised for rapid laboratory and on-site BNp detection. This assay is highly specific, rapid and as sensitive as qPCR. It was validated according to European and Mediterranean Plant Protection Organisation recommendations. In addition, 286 grapevine leaf samples from the 2015 growing season were tested with this new real-time LAMP assay and an assay previously developed for detection of Flavescence dorée phytoplasma (FDp). These LAMP assays for detection of both BNp and FDp used without any DNA extraction step, which is a required step for qPCR analysis, were comparably effective to qPCR, and positive results were obtained in less than 35 min. real-time LAMP grapevine yellows phytoplasma validation true false true Angleški jezik Ni določen Neznano 2024-07-24 03:45:59 2024-07-24 14:56:11 2024-11-27 08:49:20 0000-00-00 00:00:00 2017 0 0 str. 75-84 iss. 1 Vol. 148 2017 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2017-01-01 581.2 0929-1873 10.1007/s10658-016-1070-z 4088399 RAZ_Kogovsek_Polona_2017.pdf RAZ_Kogovsek_Polona_2017.pdf 1 11C1F9D71E4A5550EF502263A98754A7 532e2d21bb99ecb5e3df9c4b7e43e590187378f35870877c0c4f7f9320ea4515 b1d8d35a-49ab-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27367 https://doi.org/10.1007/s10658-016-1070-z 0 a94007a6-49ab-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27366 Nacionalni inštitut za biologijo Kmetijski inštitut Slovenije 0 0 0