| Title: | Impact of deoxynivalenol and zearalenone as single and combined treatment on DNA, cell cycle and cell proliferation in HepG2 cells |
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| Authors: | ID Domijan, Ana-Marija (Author)
ID Hercog, Klara (Author)
ID Štampar, Martina (Author)
ID Gajski, Goran (Author)
ID Gerić, Marko (Author)
ID Sokolović, Marijana (Author)
ID Žegura, Bojana (Author) |
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| Files: |  URL - Source URL, visit https://doi.org/10.3390/ijms24044082
 PDF - Presentation file, download (1,60 MB)
MD5: F67289B39C9B1E050FF44CE2FB9F0CC8
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | The study aimed to investigate toxicity and the mechanism of toxicity of two Fusarium mycotoxins, deoxynivalenol (DON) and zearalenone (ZEA). DON and ZEA were applied to HepG2 cells as single compounds and in combination at low environmentally relevant concentrations. HepG2 cells were exposed to DON (0.5, 1, and 2 µM), ZEA (5, 10, and 20 µM) or their combinations (1 µM DON + 5 µM ZEA, 1 µM DON + 10 µM ZEA and 1 µM DON + 20 µM ZEA) for 24 h and cell viability, DNA damage, cell cycle and proliferation were assessed. Both mycotoxins reduced cell viability, however, combined treatment with DON and ZEA resulted in higher reduction of cell viability. DON (1 µM) induced primary DNA damage, while DON (1 µM) in combination with higher ZEA concentrations showed antagonistic effects compared to DON alone at 1 µM. DON arrested HepG2 cells in G2 phase and significantly inhibited cell proliferation, while ZEA had no significant effect on cell cycle. The combined treatment with DON and ZEA arrested cells in G2 phase to a higher extend compared to treatment with single mycotoxins. Potentiating effect observed after DON and ZEA co-exposure at environmentally relevant concentrations indicates that in risk assessment and setting governments’ regulations, mixtures of mycotoxins should be considered. |
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| Keywords: | mycotoxins, comet assay, flow cytometry, co-exposure, food monitoring |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 17.02.2023 |
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| Year of publishing: | 2023 |
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| Number of pages: | str. [1]-13 |
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| Numbering: | Vol. 24, iss. 4 |
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| PID: | 20.500.12556/DiRROS-19266  |
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| UDC: | 576 |
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| ISSN on article: | 1422-0067 |
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| DOI: | 10.3390/ijms24044082 |
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| COBISS.SI-ID: | 142398979 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 21. 2. 2023;
Soavtorji: Klara Hercog, Martina Štampar, Goran Gajski, Marko Gerić, Marijana Sokolović and Bojana Žegura;
Št. članka 4082;
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| Publication date in DiRROS: | 12.07.2024 |
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| Views: | 466 |
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| Downloads: | 232 |
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