20.500.12556/DiRROS-19266 Impact of deoxynivalenol and zearalenone as single and combined treatment on DNA, cell cycle and cell proliferation in HepG2 cells The study aimed to investigate toxicity and the mechanism of toxicity of two Fusarium mycotoxins, deoxynivalenol (DON) and zearalenone (ZEA). DON and ZEA were applied to HepG2 cells as single compounds and in combination at low environmentally relevant concentrations. HepG2 cells were exposed to DON (0.5, 1, and 2 µM), ZEA (5, 10, and 20 µM) or their combinations (1 µM DON + 5 µM ZEA, 1 µM DON + 10 µM ZEA and 1 µM DON + 20 µM ZEA) for 24 h and cell viability, DNA damage, cell cycle and proliferation were assessed. Both mycotoxins reduced cell viability, however, combined treatment with DON and ZEA resulted in higher reduction of cell viability. DON (1 µM) induced primary DNA damage, while DON (1 µM) in combination with higher ZEA concentrations showed antagonistic effects compared to DON alone at 1 µM. DON arrested HepG2 cells in G2 phase and significantly inhibited cell proliferation, while ZEA had no significant effect on cell cycle. The combined treatment with DON and ZEA arrested cells in G2 phase to a higher extend compared to treatment with single mycotoxins. Potentiating effect observed after DON and ZEA co-exposure at environmentally relevant concentrations indicates that in risk assessment and setting governments’ regulations, mixtures of mycotoxins should be considered. mycotoxins comet assay flow cytometry co-exposure food monitoring mikotoksini deoxynivalenol zearalenone citotoksičnost genotoksičnost celični cikel true false true Angleški jezik Slovenski jezik Neznano 2024-07-12 03:38:36 2024-07-12 08:09:32 2024-07-13 03:49:22 0000-00-00 00:00:00 2023 0 0 Nasl. z nasl. zaslona; Opis vira z dne 21. 2. 2023; Soavtorji: Klara Hercog, Martina Štampar, Goran Gajski, Marko Gerić, Marijana Sokolović and Bojana Žegura; Št. članka 4082; str. [1]-13 iss. 4 Vol. 24 Feb. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-02-17 576 1422-0067 10.3390/ijms24044082 142398979 https://doi.org/10.3390/ijms24044082 1 751611c8-3fef-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26526 RAZ_Domijan_Ana-Marija_2023.pdf RAZ_Domijan_Ana-Marija_2023.pdf 1 F67289B39C9B1E050FF44CE2FB9F0CC8 46e14d6f3c6599a201280b7a489115b1b39277e4b035b73158551d1e5cfc0282 a8ca6e2f-4015-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26548 Nacionalni inštitut za biologijo 0 0 0