| Title: | Assessment of differential expression of oncogenes in adenocarcinoma of stomach with fluorescent labeling and simultaneous amplification of gene transcripts |
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| Authors: | ID Rajčević, Uroš (Author)
ID Hudler, Petra (Author)
ID Mijovski, Gordan (Author)
ID Gorjanc, Gregor (Author)
ID Hölzl, Georg (Author)
ID Repše, Stanislav (Author)
ID Juvan, Robert (Author)
ID Kovač, Milena (Author)
ID Huber, Christian G. (Author)
ID Komel, Radovan (Author) |
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| Files: |  PDF - Presentation file, download (86,60 KB)
MD5: D31A7E12C727D1E84C44783B435BC213
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  OI - Institute of Oncology
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| Abstract: | Background. Gastric cancer is one of the leading malignancies with a poor prognosis and low survival rates. Although the mechanisms underlying its development are still unknown, there is a consensus that genetic instability, inactivation of tumor suppressor genes and over-expression of oncogenes are involved in the early and late stages of gastric carcinogenesis. In the present study we wanted to display differential expression of seven oncogenes,namely CCNE1, EGF, ERBB3, FGF4, HRG1, HGFR and TDGF1. Patients and methods. We employed a method based on the multiplex reverse transcription polymerase chain (RT-PCR) method with a fluorescence detection. Results. More than half of patients (74.3%) out of total 74 with gastric adenocarcinoma had over-expressed at least one oncogene, with the exception of FGF4, which was expressed in tumor tissue of less than one third of patients. 56.8% of the patients patients showed over-expression of two or more oncogenes. Conclusions. Patients with precancerous lesions had elevated levels of TDGF1 or cripto-1 (64.9%) and CCNE1 (57.1%), suggesting that they could be used as markers for an early detection of malignant changes in stomach. Finally, the fluorescent multiplex RT-PCR method could be of value for rapid assessment of oncogene mRNA levels in small samples of tumor or precancerous biopsies. |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.01.2007 |
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| Publisher: | Association of Radiology and Oncology |
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| Year of publishing: | 2007 |
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| Number of pages: | str. 174-182 |
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| Numbering: | Letn. 41, št. 4 |
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| Source: | Ljubljana |
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| PID: | 20.500.12556/DiRROS-18220  |
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| UDC: | 616-006 |
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| ISSN on article: | 1318-2099 |
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| COBISS.SI-ID: | 3864346 |
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| Copyright: | by Authors |
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| Note: | Soavtorji: Petra Hudler, Goran Mijovski, gregor Gorjanc, Georg Hoelzl, Stanislav Repše, Robert Juvan, Milena Kovač, Christian G. Huber, Radovan Komel;
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| Publication date in DiRROS: | 20.02.2024 |
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| Views: | 1050 |
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| Downloads: | 325 |
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