20.500.12556/DiRROS-18220 Assessment of differential expression of oncogenes in adenocarcinoma of stomach with fluorescent labeling and simultaneous amplification of gene transcripts Background. Gastric cancer is one of the leading malignancies with a poor prognosis and low survival rates. Although the mechanisms underlying its development are still unknown, there is a consensus that genetic instability, inactivation of tumor suppressor genes and over-expression of oncogenes are involved in the early and late stages of gastric carcinogenesis. In the present study we wanted to display differential expression of seven oncogenes,namely CCNE1, EGF, ERBB3, FGF4, HRG1, HGFR and TDGF1. Patients and methods. We employed a method based on the multiplex reverse transcription polymerase chain (RT-PCR) method with a fluorescence detection. Results. More than half of patients (74.3%) out of total 74 with gastric adenocarcinoma had over-expressed at least one oncogene, with the exception of FGF4, which was expressed in tumor tissue of less than one third of patients. 56.8% of the patients patients showed over-expression of two or more oncogenes. Conclusions. Patients with precancerous lesions had elevated levels of TDGF1 or cripto-1 (64.9%) and CCNE1 (57.1%), suggesting that they could be used as markers for an early detection of malignant changes in stomach. Finally, the fluorescent multiplex RT-PCR method could be of value for rapid assessment of oncogene mRNA levels in small samples of tumor or precancerous biopsies. onkologija rak želodca genetika neoplazem prepis polimeraz onkogeni true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-02-20 13:46:56 2024-02-20 13:46:57 2024-02-23 03:38:09 0000-00-00 00:00:00 2007 0 0 Soavtorji: Petra Hudler, Goran Mijovski, gregor Gorjanc, Georg Hoelzl, Stanislav Repše, Robert Juvan, Milena Kovač, Christian G. Huber, Radovan Komel; str. 174-182 št. 4 Letn. 41 2007 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2007-01-01 616-006 1318-2099 3864346 RAZ_Rajcevic_Uros_2007.pdf RAZ_Rajcevic_Uros_2007.pdf 1 D31A7E12C727D1E84C44783B435BC213 ac4beb69fcff55bd4224a499d1fe68c1e5d4dac619f5ec9c7391a00bda8f70d8 f9d3a37b-cfe5-11ee-93e4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24746 Onkološki inštitut Ljubljana 0 0 0