| Title: | Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex |
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| Authors: | ID Minias, Alina (Author)
ID Żukowska, Lidia (Author)
ID Lach, Jakub (Author)
ID Jagielski, Tomasz (Author)
ID Strapagiel, Dominik (Author)
ID Kim, Su-Young (Author)
ID Koh, Won-Jung (Author)
ID Adam, Heather (Author)
ID Bittner, Ruth (Author)
ID Truden, Sara, Klinika Golnik (Author)
ID Žolnir-Dovč, Marija, Klinika Golnik (Author)
ID Dziadek, Jarosław (Author) |
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| Files: |  PDF - Presentation file, download (1,23 MB)
MD5: A46DF102BA18581FE05EFC69C4D9A26E
 ZIP - Supplement, download (4,12 MB)
MD5: 19FA2F9CEA4C9546680DD62253778CEB
 URL - Source URL, visit https://www.nature.com/articles/s41598-020-73607-x.pdf
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  UKPBAG - University Clinic of Respiratory and Allergic Diseases Golnik
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| Abstract: | Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as emerging human pathogens. MABC consists of 3 subspecies - M. abscessus subsp. bolletti, M. abscessus subsp. massiliense and M. abscessus subsp. abscessus. Here we present a novel method for subspecies differentiation of M. abscessus named Subspecies-Specific Sequence Detection (SSSD). This method is based on the presence of signature sequences present within the genomes of each subspecies of MABC. We tested this method against a virtual database of 1505 genome sequences of MABC. Further, we detected signature sequences of MABC in 45 microbiological samples through DNA hybridization. SSSD showed high levels of sensitivity and specificity for differentiation of subspecies of MABC, comparable to those obtained by rpoB sequence typing. |
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| Keywords: | Mycobacterium abscessus complex, nontuberculous mycobacteria, diagnosis |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Place of publishing: | Velika Britanija |
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| Publisher: | Springer Nature |
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| Year of publishing: | 2020 |
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| Number of pages: | str. 1-9 |
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| Numbering: | Vol. 10 |
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| PID: | 20.500.12556/DiRROS-12693  |
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| UDC: | 579 |
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| ISSN on article: | 2045-2322 |
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| DOI: | 10.1038/s41598-020-73607-x |
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| COBISS.SI-ID: | 38347779 |
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| Copyright: | © The Author(s) 2020 |
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| Note: | Nasl. z nasl. zaslona;
Soavtorici iz Slovenije: Sara Truden, Manca Žolnir-Dovč;
Opis vira z dne 19. 11. 2020;
Article no. 16415;
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| Publication date in DiRROS: | 23.11.2020 |
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| Views: | 12474 |
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| Downloads: | 1309 |
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| Metadata: |    |
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