20.500.12556/DiRROS-12693 Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as emerging human pathogens. MABC consists of 3 subspecies - M. abscessus subsp. bolletti, M. abscessus subsp. massiliense and M. abscessus subsp. abscessus. Here we present a novel method for subspecies differentiation of M. abscessus named Subspecies-Specific Sequence Detection (SSSD). This method is based on the presence of signature sequences present within the genomes of each subspecies of MABC. We tested this method against a virtual database of 1505 genome sequences of MABC. Further, we detected signature sequences of MABC in 45 microbiological samples through DNA hybridization. SSSD showed high levels of sensitivity and specificity for differentiation of subspecies of MABC, comparable to those obtained by rpoB sequence typing. Mycobacterium abscessus complex nontuberculous mycobacteria diagnosis kompleks Mycobacterium abscessus netuberkulozne mikobakterije diagnostika true false true Springer Nature Angleški jezik Ni določen © The Author(s) 2020 Neznano 2020-11-23 13:04:07 2020-11-23 13:04:08 2022-08-18 03:38:53 0000-00-00 00:00:00 2020 0 Velika Britanija 0 Nasl. z nasl. zaslona; Soavtorici iz Slovenije: Sara Truden, Manca Žolnir-Dovč; Opis vira z dne 19. 11. 2020; Article no. 16415; str. 1-9 Vol. 10 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 2045-2322 10.1038/s41598-020-73607-x 38347779 18727432 RAZ_Minias_Alina_i2020.pdf RAZ_Minias_Alina_i2020.pdf 1 A46DF102BA18581FE05EFC69C4D9A26E 1f40a95c50d665419eb47bd3f86d3f6c894c02e05d488d773dc5ff68d8c1c7ef 87a300cd-17b6-11ed-b6b8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=17202 RAZ_Minias_Alina_i2020.zip RAZ_Minias_Alina_i2020.zip 1 19FA2F9CEA4C9546680DD62253778CEB b4ab93a1a64d73ff079e6df422fdb84d101b450dd0e93731d8edfb25fc91fe3e 87b3aab7-17b6-11ed-b6b8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=17203 https://www.nature.com/articles/s41598-020-73607-x.pdf 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=15454 Univerzitetna klinika za pljučne bolezni in alergijo Golnik 0 0 0