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Title:Transcription factors gene expression in chronic rhinosinusitis with and without nasal polyps
Authors:Soklič, Tanja (Author)
Rijavec, Matija (Author)
Šilar, Mira (Author)
Koren, Ana (Author)
Kern, Izidor (Author)
Hočevar-Boltežar, Irena (Author)
Korošec, Peter (Author)
Tipology:1.01 - Original Scientific Article
Organisation:Logo UKPBAG - University Clinic of Respiratory and Allergic Diseases Golnik
Abstract:Background. Chronic rhinosinusitis (CRS) current therapeutic approaches still fail in some patients with severe persistent symptoms and recurrences after surgery. We aimed to evaluate the master transcription factors gene expression levels of T cell subtypes in chronic rhinosinusitis with nasal polyps (CRSwNP) and chronic rhinosinusitis without nasal polyps (CRSsNP) that could represent new, up-stream targets for topical DNAzyme treatment. Patients and methods. Twenty-two newly diagnosed CRS patients (14 CRSwNP and 8 CRSsNP) were prospectively biopsied and examined histopathologically. Gene expression levels of T-box transcription factor (T-bet, TBX21), GATA binding protein 3 (GATA3), Retinoic acid-related orphan receptor C (RORC) and Forkhead box P3 (FOXP3) were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). Results. Eosinophilic CRSwNP was characterized by higher level of GATA3 gene expression compared to noneosinophilic CRSwNP, whereas there was no difference in T-bet, RORC and FOXP3 between eosinophilic and noneosinophilic CRSwNP. In CRSsNP, we found simultaneous upregulation of T-bet, GATA3 and RORC gene expression levels in comparison to CRSwNP; meanwhile, there was no difference in FOXP3 gene expression between CRSwNP and CRSsNP. Conclusions. In eosinophilic CRSwNP, we confirmed the type 2 inflammation by elevated GATA3 gene expression level. In CRSsNP, we unexpectedly found simultaneous upregulation of T-bet and GATA3 that is currently unexplained; however, it might originate from activated CD8+ cells, abundant in nasal mucosa of CRSsNP patients. The elevated RORC in CRSsNP could be part of homeostatic nasal immune response that might be better preserved in CRSsNP patients compared to CRSwNP patients. Further data on transcription factors expression rates in CRS phenotypes are needed.
Keywords:sinusitis, nasal polyps, Th1 cells, Th2 cells, Th17 cells, transcription factors, chronic rhinosinusitis
Year of publishing:2019
Publisher:Association of Radiology and Oncology
COBISS_ID:2048522353 Link is opened in a new window
ISSN on article:1581-3207
OceCobissID:784507 Link is opened in a new window
DOI:10.2478/raon-2019-0029 Link is opened in a new window
Note:Soavtorji: Matija Rijavec, Mira Silar, Ana Koren, Izidor Kern, Irena Hocevar-Boltezar, Peter Korosec; Nasl. z nasl. zaslona; Opis vira z dne 26. 7. 2019;
Files:.pdf PDF - Presentation file, download (698,54 KB)
URL URL - Source URL, visit
Journal:Radiology and oncology
Association of Radiology and Oncology
Rights:© 2019 Tanja Kosak Soklic, Matija Rijavec, Mira Silar, Ana Koren, Izidor Kern, Irena Hocevar- Boltezar, Peter Korosec
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License:CC BY-NC-ND 3.0, Creative Commons Attribution Non-Commercial No Derivatives 3.0
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Licensing start date:17.07.2019