| Title: | Transcription factors gene expression in chronic rhinosinusitis with and without nasal polyps |
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| Authors: | ID Soklič, Tanja (Author)
ID Rijavec, Matija, Klinika Golnik, Biotehniška fakulteta UL (Author)
ID Šilar, Mira, Klinika Golnik (Author)
ID Koren, Ana, Klinika Golnik (Author)
ID Kern, Izidor, Klinika Golnik (Author)
ID Hočevar-Boltežar, Irena (Author)
ID Korošec, Peter, Klinika Golnik (Author) |
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| Files: |  PDF - Presentation file, download (698,54 KB)
MD5: D70A5366437474F51F17AE72616F3CCD
 URL - Source URL, visit https://www.degruyter.com/downloadpdf/j/raon.ahead-of-print/raon-2019-0029/raon-2019-0029.pdf
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  UKPBAG - University Clinic of Respiratory and Allergic Diseases Golnik
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| Abstract: | Background. Chronic rhinosinusitis (CRS) current therapeutic approaches still fail in some patients with severe persistent symptoms and recurrences after surgery. We aimed to evaluate the master transcription factors gene expression levels of T cell subtypes in chronic rhinosinusitis with nasal polyps (CRSwNP) and chronic rhinosinusitis without nasal polyps (CRSsNP) that could represent new, up-stream targets for topical DNAzyme treatment. Patients and methods. Twenty-two newly diagnosed CRS patients (14 CRSwNP and 8 CRSsNP) were prospectively biopsied and examined histopathologically. Gene expression levels of T-box transcription factor (T-bet, TBX21), GATA binding protein 3 (GATA3), Retinoic acid-related orphan receptor C (RORC) and Forkhead box P3 (FOXP3) were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). Results. Eosinophilic CRSwNP was characterized by higher level of GATA3 gene expression compared to noneosinophilic CRSwNP, whereas there was no difference in T-bet, RORC and FOXP3 between eosinophilic and noneosinophilic CRSwNP. In CRSsNP, we found simultaneous upregulation of T-bet, GATA3 and RORC gene expression levels in comparison to CRSwNP; meanwhile, there was no difference in FOXP3 gene expression between CRSwNP and CRSsNP. Conclusions. In eosinophilic CRSwNP, we confirmed the type 2 inflammation by elevated GATA3 gene expression level. In CRSsNP, we unexpectedly found simultaneous upregulation of T-bet and GATA3 that is currently unexplained; however, it might originate from activated CD8+ cells, abundant in nasal mucosa of CRSsNP patients. The elevated RORC in CRSsNP could be part of homeostatic nasal immune response that might be better preserved in CRSsNP patients compared to CRSwNP patients. Further data on transcription factors expression rates in CRS phenotypes are needed. |
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| Keywords: | sinusitis, nasal polyps, Th1 cells, Th2 cells, Th17 cells, transcription factors, chronic rhinosinusitis |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Place of publishing: | Slovenija |
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| Publisher: | Association of Radiology and Oncology |
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| Year of publishing: | 2019 |
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| Number of pages: | str. 323-330 |
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| Numbering: | Vol. 53, iss. 3 |
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| PID: | 20.500.12556/DiRROS-12541  |
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| UDC: | 616.21 |
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| ISSN on article: | 1581-3207 |
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| DOI: | 10.2478/raon-2019-0029 |
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| COBISS.SI-ID: | 2048522353 |
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| Copyright: | © 2019 Tanja Kosak Soklic, Matija Rijavec, Mira Silar, Ana Koren, Izidor Kern, Irena Hocevar- Boltezar, Peter Korosec |
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| Note: | Soavtorji: Matija Rijavec, Mira Silar, Ana Koren, Izidor Kern, Irena Hocevar-Boltezar, Peter Korosec;
Nasl. z nasl. zaslona;
Opis vira z dne 26. 7. 2019;
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| Publication date in DiRROS: | 09.10.2020 |
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| Views: | 1474 |
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| Downloads: | 815 |
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