21. Analysis of putative apoplastic effectors from the nematode, Globodera rostochiensis, and identification of an expansin-like protein that can induce and suppress host defensesBarbara Gerič Stare, Shawkat Ali, Maxime Magne, Shiyan Chen, Olivier Côte, Natasa Obradovic, Lubna Jamshaid, Xiaohong Wang, Guy Bélair, Peter Moffett, 2015, original scientific article Keywords: nematode, expansin-like protein, expansin, potato cyst nematode, potato pest, parasitic nematodes, plant celular function
Published in DiRROS: 06.09.2022; Views: 475; Downloads: 205
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22. Uporaba naprednih tehnologij za celostno obvladovanje ogorčic koreninskih šišk (Meloidogyne spp., Nematoda: Meloidogynidae)Nik Susič, 2020, doctoral dissertation Abstract: Tropske vrste ogorčic koreninskih šišk (RKN) so izredno polifagni škodljivci v kmetijstvu, kjer povzročajo velike izgube pridelka. Nekatere vrste, kot so Meloidogyne ethiopica, M. inornata in M. luci (MEG), je zaradi visoke sorodnosti težavno razlikovati. Sekvencirali smo 7 izolatov ogorčic iz skupine MEG ter po zlaganju zaporedij genomov določili filogenetsko umestitev v rod. Zloženi genom ogorčice M. luci SI-Smartno V13 je s 327 kontigi, N50 dolžini kontigov 1,7 Mb in končni dolžini zloženega zaporedja 209,2 Mb, trenutno najpopolnejši javno dostopen genom RKN. Filogenetska analiza je pokazala na umestitev vseh izolatov iz skupine MEG v enoten monofiletski klad, vrsta M. luci se je bistveno razlikovala od vrst M. ethiopica in M. inornata. Preizkušali smo uporabnost hiperspektralnega slikanja za zgodnje razločevanje biotskega stresa (napad RKN) od abiotskega stresa (suša) pri rastlinah paradižnika. Z analizo hiperspektralnih posnetkov v območju spektra 400-2500 nm je bilo mogoče razlikovati med dobro zalitimi ter sušnimi rastlinami s 100 % natančnostjo, z 90-100 % natančnostjo pa je bilo mogoče razlikovati med zdravimi in napadenimi rastlinami. Ovrednotili smo nematicidno aktivnost in analizirali genoma bakterij Bacillus firmus I-1582 in Bacillus sp. ZZV12-4809, v katerih smo našli številne potencialne dejavnike virulence. V lončnih poskusih in na mikroparcelah je sev I-1582 zmanjšal število ogorčic M. luci za 51-53 % v primerjavi s kontrolo. Bakterije I-1582 so delovale nematicidno ter spodbudile rast rastlin, kar smo pokazali z meritvami morfologije rastlin, relativne vsebnosti klorofila, vsebnostjo elementov in analizo hiperspektralnih posnetkov. Z analizo hiperspektralnih posnetkov z nadzorovano klasifikacijo smo uspešno razlikovali med rastlinami tretiranimi z B. firmus in rastlinami brez bakterij – v lončnem poskusu smo dosegli 97,4 % uspešnost, na mikroparcelah pa 96,3 %.
Keywords: ogorčice koreninskih šišk, filogenomika, hiperspektralno slikanje, biotično varstvo rastlin, Meloidogyne spp., Bacillus firmus, doktorske disertacije
Published in DiRROS: 06.09.2022; Views: 689; Downloads: 219
Link to full text |
23. Potential of cover crops to control arthropod pests in organic viticultureAurora Ranca, Anne-Laure Fragnière, Sergiu Ayar Ene, Špela Modic, Vittorio Rossi, Tito Caffi, Jaka Razinger, Eugenia Rodrigo Santamalia, Daniel Wipf, Patrik Kehrli, original scientific article Keywords: conservation biological control, pest control, functional diversity
Published in DiRROS: 06.09.2022; Views: 468; Downloads: 256
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25. Late blight resistance conferred by Rpi-Smira2/R8 in potato genotypes in vitro depends on the genetic backgroundEva Blatnik, Marinka Horvat, Sabina Berne, Miha Humar, Peter Dolničar, Vladimir Meglič, 2022, original scientific article Keywords: potato, potato genotype, potato R8 genotypes, in vitro infection, late blight, resistant cultivars, oomicete, Sárpo Mira
Published in DiRROS: 01.09.2022; Views: 471; Downloads: 177
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26. Histology basics and cell death detection in honeybee tissueMaja Ivana Smodiš Škerl, original scientific article Abstract: Honeybees (Apis mellifera L.) inside the hive (nurse workers and other hive bees)
and outside the hive (foragers) are exposed to climate and weather changes, various
pesticides, pathogens, and malnutrition, mainly entering through the mouth and
primarily affecting the digestive tracts of adult bees. To understand and prevent the
effects of such external and internal stressors on honeybees, one useful research
method is the immunohistochemical method. A basic protocol is described to prepare
the midgut (ventriculus) and hypopharyngeal glands (HPGs) of adult bees for
histological analysis. A detailed methodology is described to assess the level of cell
damage and distinguish necrosis from programmed cell death (apoptosis) as a natural
process of tissue regeneration. The results of adult honeybee treatment with oxalic
acid and pesticides (insecticide and acaricide) and the determination of cell death in
the ventriculus and HPGs are presented. The pros and cons of the methodology are
also discussed.
Keywords: cell death, honeybee, histology, environment
Published in DiRROS: 08.07.2022; Views: 534; Downloads: 301
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29. Microarray-based uncovering of reference genes for quantitative real-time PCR in potato tuber infected with PVYBarbara Gerič Stare, Aleš Sedlar, Vladimir Meglič, 2021, original scientific article Keywords: potato, potato tuber, tuber infection, potato virus Y, real-time PCR, PCR, polymerase chain reaction, reference genes, stored ppotato tubers, pathogens
Published in DiRROS: 31.05.2021; Views: 1322; Downloads: 300
Full text (488,30 KB) |
30. Genes associated with somatic cell count index in Brown Swiss cattleJanez Jeretina, Daša Jevšinek Skok, 2020, original scientific article Keywords: subclinical mastitis, cattle, diseases of cows, contagious pathogens, environmental pathogens, diagnostic tests, somatic cell count (SCC), inflammatory process, somatic cell count index (SCCI)
Published in DiRROS: 27.10.2020; Views: 1717; Downloads: 442
Full text (983,42 KB) |
