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4. Scanning electron microscope images of isolates of small cellular particles Phaeodactylum tricornutum grown in media supplemented with Guillard’s (F/2) marine : marine water enrichment solution, BG11 broth and Lennox LB brothApolonija Bedina Zavec, Darja Božič, Matej Hočevar, Aleš Iglič, Marko Jeran, Veronika Kralj-Iglič, Anna Romolo, 2024, published scientific conference contribution Published in DiRROS: 08.03.2024; Views: 56; Downloads: 30 Full text (13,06 MB) This document has many files! More... |
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7. Mechanisms of premature fracture in modular neck stems made of CoCrMo/Ti6Al4V and Ti6Al4V/Ti6Al4V alloyDrago Dolinar, Miro Gorenšek, Klemen Avsec, Barbara Šetina, Matej Hočevar, Matjaž Godec, Borut Žužek, Mojca Debeljak, Monika Jenko, John T. Grant, Boštjan Kocjančič, 2023, original scientific article Abstract: In this paper, we present the mechanisms of premature fracture of modular neck stems in two case studies: (I) when the neck and stem are both made of the same Ti6Al4V alloy, and (II) when the neck and stem are made from two different alloys, CoCrMo and Ti6Al4V alloy. Our study integrates two orthopedic patients who have undergone primary uncemented THA for usual indications in two orthopedic centers (Community Health Centre and University Medical Centre). Both centers are part of the national public health care system. Both surgeries were performed by two skilled orthopedic surgeons with more than 10 years of experience in THA. The survivorship of the modular neck of cast CoCrMo alloy was 24 months. The survivorship of the modular neck from Ti6Al4V alloy was 84 months. Multivariate analyses were performed to assess the differences in the fretting, corrosion, and fatigue of the two prematurely failed modular neck stems: stereo light microscopy (SLM), scanning electron microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and electron backscatter diffraction (EBSD). Patient demographic information, including sex, age, body mass index, survivorship of implants, and reason for the revision, was collected from medical records. We found that fretting and fatigue occurred on both neck-stem retrievals due to additional galvanic corrosion, but the CoCrMo/Ti6Al4V alloy system suffered more corrosion due to additional galvanic corrosion and fractured earlier than the Ti6Al4V/Ti6Al4V metal alloy system. Both metallic alloy systems used in this application are known to be highly corrosion-resistant, but the bio-tribo-corrosion processes need to be understood in detail and characterized so that appropriate improvements in design and materials can be made. Keywords: total hip arthroplasty, modular neck, Ti6Al4V alloy, CoCrMo alloy, corrosion Published in DiRROS: 07.02.2024; Views: 163; Downloads: 86 Full text (5,12 MB) This document has many files! More... |
8. Small cellular particles from European spruce needle homogenateMarko Jeran, Anna Romolo, Vesna Spasovski, Matej Hočevar, Urban Novak, Roman Štukelj, Vid Šuštar, Matic Kisovec, Apolonija Bedina Zavec, Ksenija Kogej, Aleš Iglič, Polonca Trebše, Veronika Kralj-Iglič, 2023, original scientific article Keywords: Picea abies, spruce needles, extracellular particles, extracellular vesicles, small cellular particles, interferometric light microscopy, exosomes, cell-to-cell communication, drug delivery Published in DiRROS: 07.02.2024; Views: 121; Downloads: 77 Full text (2,49 MB) This document has many files! More... |
9. Inflammatory, oxidative stress and small cellular particle response in HUVEC induced by debris from endoprosthesis processingZala Jan, Matej Hočevar, Veno Kononenko, Sara Michelini, Neža Repar, Maja Caf, Boštjan Kocjančič, Drago Dolinar, Slavko Kralj, Darko Makovec, Aleš Iglič, Damjana Drobne, Monika Jenko, Veronika Kralj-Iglič, 2023, original scientific article Keywords: endoprosthesis failure, corundum, cytokines, reactive oxygen species, lipid droplets Published in DiRROS: 02.02.2024; Views: 118; Downloads: 44 Full text (4,34 MB) This document has many files! More... |
10. Morphological characteristics of young and old murine hematopoietic stem cell niches, as modeled in vitroMojca Justin, Ema Rogac Randl, Veno Kononenko, Matej Hočevar, Damjana Drobne, Primož Rožman, 2023, original scientific article Abstract: The hematopoietic stem cell (HSC) niche undergoes detrimental changes with age. The molecular differences between young and
old niches are well studied and understood; however, young and old niches have not yet been extensively characterized in terms of
morphology. In the present work, a 2D stromal model of young and old HSC niches isolated from bone marrow was investigated
using light and scanning electron microscopy (SEM) to characterize cell density after one, two, or three weeks of culturing, cell
shape, and cell surface morphological features. Our work is aimed at identifying morphological differences between young and
old niche cells that could be used to discriminate between their respective murine HSC niches. The results show several age-
specific morphological characteristics. The old niches differ from the young ones in terms of lower cell proliferating capacity,
increased cell size with a flattened appearance, increased number of adipocytes, and the presence of tunneling nanotubes. In
addition, proliferating cell clusters are present in the young niches but not in the old niches. Together, these characteristics
could be used as a relatively simple and reliable tool to discriminate between young and old murine HSC niches and as a
complementary approach to imaging with specific cellular markers. Keywords: bone marrow, hematoopetic stem cell niche, aging, adipocytes, scanning electron microscopy Published in DiRROS: 26.01.2024; Views: 152; Downloads: 67 Full text (2,47 MB) This document has many files! More... |