11. Effect of electroporation on radiosensitization with cisplatin in two cell lines with different chemo- and radiosensitivitySimona Kranjc Brezar, Maja Čemažar, Alenka Grošel, Živa Pipan Tkalec, Gregor Serša, 2003, original scientific article Abstract: Aim. Radiosensitization with cisplatin can be enhanced by electroporation of cells and tumours. The aim of this study was to extend our previous studies ontwo carcinoma tumour models with different chemo-and radiosensitivity in order to evaluate whether this treatment is effective also on less chemo-and radiosensitive tumour cells. Materials and methods. This in vitro study was performed on carcinoma SCK and EAT-E cells. The cytotoxicity of three-modalitytreatment consisting of cisplatin, electroporation and irradiation was determined by the clonogenic assay. Results. The radiosensitizing effect of cisplatin on the two cell lines was greatly enhanced by electroporation. By this combined treatment, less chemo and radiosensitive EAT-E cells were rendered as sensitive as more chemo and radiosensitive SCK cells. Conclusion. The enhancement of cisplatin-induced radiosensitization of cells by electroporation could be beneficially used in the treatment of intrinsically less chemo- and radiosensitive tumours.
Published in DiRROS: 06.02.2024; Views: 143; Downloads: 32
 Full text (144,48 KB) |
12. Effects of 5-Gy irradiation on fertility and mating behaviour of Nezara viridula (Heteroptera: Pentatomidae)Alenka Žunič Kosi, Andrej Čokl, Gregor Serša, 2002, original scientific article Abstract: Background. The polyphagous and cosmopolitan species Nezara viridula is one ofthe most important insect pests. The sterile insect technique (SIT) is a pest control strategy that involves sterilising males by exposing them to ionising radiation. Sterile males, released into wild population, mate with females, but eggs are not fertilised and the population gradually declines. Exposing insects to radiation during their growth stage might require lower sterilising dose. The aim of our study was to test whether 5-Gy irradiation of5th instar nymphs significantly affects: (1) moulting and further development of the irradiated nymphs, (2) the male's and female's reproduetivesystem and (3) the mating competitiveness of treated males, with special focus on vibrational communication. Methods: The 5th instar nymphs were irradiated with 5 Gy using X ray generator and monitored daily. Results: The observed effects of irradiation were: prolonged moulting increased mortality during development and during the first day of adult life, decreasedmales to females ratio, decreased fecundity, egg production, proportion of fertile eggs and progeny survival. The reaction of a male to stimulation with the model female calling song was tested. The irradiated and non-irradiated males responded to stimulation with emission of the eourtship song (MCrS). Temporal parameters of MCrS emitted by non-irradiated males differed when compared with those of irradiated ones. Conclusions: The 5-Gy irradiation of 5th instar nymphs did not affect mating behaviour. However since the irradiation during growth stage decreased the fertility and fecundity of emerged adults, this technique, in combination with certain othersuppression teehniques, could be a successful control strategy for management of Nezara viridula. On the other hand observed effects on moulting and further development of the irradiated nymphs could decrease the efficiencyand application of this strategy.
Published in DiRROS: 31.01.2024; Views: 150; Downloads: 36
Full text (93,75 KB) |
13. Fine particulate matter (PM2.5) exposure assessment among active daily commuters to induce behaviour change to reduce air pollutionAnja Ilenič, Alenka Mauko Pranjić, Nina Zupančič, Radmila Milačič, Janez Ščančar, 2023, original scientific article Abstract: Fine particulate matter (PM2.5), a detrimental urban air pollutant primarily emitted by traffic and biomass burning, poses disproportionately significant health risks at relatively limited exposure during commuting. Previous studies have mainly focused on fixed locations when assessing PM2.5 exposure, while neglecting pedestrians and cyclists, who often experience higher pollution levels. In response, this research aimed to independently validate the effectiveness of bicycle-mounted low-cost sensors (LCS) adopted by citizens, evaluate temporal and spatial PM2.5 exposure, and assess associated health risks in Ljubljana, Slovenia. The LCS quality assurance results, verified by co-location field tests by air quality monitoring stations (AQMS), showed comparable outcomes with an average percentage difference of 21.29 %, attributed to humidity-induced nucleation effects. The colder months exhibited the highest air pollution levels (μ = 32.31 μg/m3) due to frequent thermal inversions and weak wind circulation, hindering vertical air mixing and the adequate dispersion of pollutants. Additionally, PM2.5 levels in all sampling periods were lowest in the afternoon (μ = 12.09 μg/m3) and highest during the night (μ = 61.00 μg/m3) when the planetary boundary layer thins, leading to the trapping of pollutants near the surface, thus significantly affecting diurnal and seasonal patterns. Analysis of exposure factors revealed that cyclists were approximately three times more exposed than pedestrians. However, the toxicological risk assessment indicated a minimal potential risk of PM2.5 exposure. The collaborative integration of data from official AQMS and LCS can enhance evidence-based policy-making processes and facilitates the realignment of effective regulatory frameworks to reduce urban air pollution.
Keywords: air pollution monitoring, PM2.5 exposure, citizen science, bicycle-mounted low-cost sensors
Published in DiRROS: 30.01.2024; Views: 181; Downloads: 73
Full text (2,57 MB)
This document has many files! More... |
14. Cell electropermeabilization to small molecules in vitro : control by pulse parametersAlenka Maček Lebar, Damijan Miklavčič, 2001, original scientific article Abstract: A systematic study concerning the role of the different electric field parameters (pulse number, duration and amplitude) on electropermeabilization of DC3F cells to small molecules (propidium iodide) and on cell viability is presented. Cell permeabilization and viability dependence on the pulse amplitude was determined by twenty different sets of electrical parameters. The number of pulses varied between 1 and 64 and pulse duration between 20 micros and 1 ms. The most important parameter was the pulse amplitude because at triggered the electropermeabilization process and the process of cell death. Either in the case of electropermeabilization as well as in the case ofcell viability experiments, the parameter Uso (the pulse amplitude leading to permeabilization or to the death of 50% of cell population) was not changedif the set of electrical parameters consisted of more than 16 pulses. This was independent of the pulse duration. The efficiency of permeabilizationwas enhanced by using of longer pulses. Such a systematic study of the influence of different electric field parameters on electropermeabilization and cell viability may serve as a base for optimization of the electropermeabilization conditions for different applications.
Published in DiRROS: 25.01.2024; Views: 183; Downloads: 40
Full text (508,35 KB) |
15. Strictures of the male urethra: how to perform and evaluate radiourethrography and sonourethrography to avoid mistakesDarja Babnik-Peskar, Alenka Višnar-Perovič, 2000, original scientific article Abstract: Introduction. Precise evaluation of the uretheral stricture localisation, length, depth and quality of wall changes are necessary for the selection of the optimal treatment to prevent recurrences. Decision for urethral dilatation, internal urethrotomy or open surgery depends on urethroscopic, radiourethrographic-RUG, and recently, according to MC Annich, also sonourethrographic-SUG results. SUG adds information about the depth and density of spongiofibrosis. Previously published findings of RUG length underestimation, compared to SUG and endoscopy, were responsible for inappropriate treatment and recurrences. In our article, we evaluate SUG as a new imaging method in the diagnosis of uretheral strictures and treatment planning, and describe both imaging methods together with usual pitfalls in performance or interpretation, possible reason for incorrect length measurements, and RUG length underestimation. Patients and methods. In the period of 20 months, we evaluated SUG compared to RUG and endoscopy in 51/130 males with suspected urethral strictures. Results. Compared to RUG, sonographywas correct in 92% radiographycally detected strictures. Length measurements by sonography were 22% shorter than radiographic lengths, which could be explained by radiographic magnification, as it was proved with wire measure placed on penis. Considering radiographic magnification, we did not find important differences in any measurement. Opposite to previously published RUG results compared to endoscopy and SUG, radiographic length underestimation was not found. Conclusions. The combination of both imaging methods provides optimal information about urethral stricture anatomy. We conclude that published radiographic length underestimation could be only a misinterpretation. (Abstract truncated at 2000 characters).
Published in DiRROS: 25.01.2024; Views: 163; Downloads: 40
Full text (1,40 MB) |
16. Ultrasound-guided aspiration biopsy of subclinical regional metastases of head and neck carcinomaAlenka Višnar-Perovič, Aleksandar Aničin, Živa Zupančič, Bojana Černelč, Alojz Šmid, Dubravka Vidmar, Milan Gorenc, 2000, original scientific article Abstract: Background. Studies concerned with the estimation of ultrasound (US) combined with ultrasound-guided aspiration biopsy (USGAB) in the detection of subclinical regional metastases from the planocellular carcinoma of head and neck are promising, but in few cases. Recently, the authors have pointed out the role of lymph node size parameters in order to decide about the use of USGAB. The aim of this study was to test the reliability of US-USGAB for the detection of subclinical regional metastases in patients with planocellular head and neck carcinomas, including the evaluation of lymph node size parameters. Patients and methods. 121 neck sides with no palpable metastases were examined by US in 77 patients with planocellular carcinomas of head and neck. Depending on the results obtained, USGAB was performed on 64 neck sides.After surgery, the results of US-USGAB were compared with histological findings of the dissected lymph nodes. Results. The study showed 77% overall sensitivity of US and USGAB, and 100% specificity, while the versal / longiyudinal diameter was the best predictor of metastatic lymph node involvement. Conclusion. The results of this study established that USGAB is an appropriate method for detection the subclinical regional metastases on neck, and should be included into routine diagnostic work up in the evaluationof the extent of head and necks carcinomas.
Published in DiRROS: 24.01.2024; Views: 162; Downloads: 40
Full text (415,17 KB) |
17. Ultrasonographic diagnnosis of obstructive ileus in a patient with Meckel's diverticulumAlenka Višnar-Perovič, Aleš Koren, 1999, original scientific article Abstract: Introduction. Despite the use of modern imaging techniques, the reliable preoperative assessment of Meckel's diverticulurn and related complications with this rare congenital anomaly of the gastrointestinal tract in adults is uncomrnon. Case presentation. This report presents the case of a 25 year old man who presented with a sudden onset of pain in the right lower abdomen and vomiting. On clinical examination the affected area was tender to palpation which revealed an elastic cylindrical formation situated deeply in the abdomen. Blumberg's sign was positive while the laboratory findings were stillwithin normal limits. An ultrasonography of the abdomen revealed an ileocaecal fluid collection containing thicker residue, which was suspicious for Meckel's diverticulum or a duplication cyst and ileus of the small bowel proximally from the formation described. Native radiogram of the abdomen in supine position has confirmed the presence of obstructive ileus at the level of the distal part of the small bowel. Surgery revealed an ileus and compression of the distal part of the small bowel due to the presence of an edematous Meckel's diverticulum. Conclusion. In view of the frequent use of ultrasonography in the evaluation of acute ahdomen, the diagnostic procedures could be rationalized and the time to surgery reduced if possible complicatioris due to Meckel's diverticulum would he considered in the differential diagnosis.
Published in DiRROS: 22.01.2024; Views: 157; Downloads: 40
Full text (651,25 KB) |
18. Influence of UV-B radiation on Norway spruce seedlings (Picea abies (L.) Karst.)Jože Bavcon, Nada Gogala, Alenka Gaberščik, 1998, original scientific article Abstract: On the basis of the hypothesis that the ultraviolet radiation is one of the main causes for damage at higher altitudes, we have monitored the effect of UVradiation on Norway spruce for two and a half years. The influence of UV-B radiation on Picea abies (L). Karst.seedlings cultured in pots in open greenhouses was examined by measuring photochemical efficiency of photosystem II, changes in chlorophyll a, b, and changes in anthocyanins. The seedlings were grown in a mixture of peat and vermiculite (4:1). We used Osram ultravitaluks bulbs as a source of UV-B radiation. In the experiment plants were treated with 21.24+-3.5 kJ/m2 and 31.9+-2.5 KJ/m2. The control plants were grown under ambient conditions in the greenhouse without artificial source of UV-B radiation. The mean yearly values were as high as 11.5+-5.2 kJ/m2. The photochemical efficiency of photosystem II (PS II) in experimental plants did not vary during the experiment. It showed obvious decrease in the winter period, due to low temperatures and physical draught. The decrease in chlorophyll a and b, was already detected after one year of treatment with simultaneous changes in a/b ration. An increase of anthocyanins amount was detected as well.
Published in DiRROS: 19.01.2024; Views: 186; Downloads: 44
Full text (1,67 MB) |
19. |
20. Resections of pelvic bone and sacrum, Ljubljana experienceJanez Novak, Marjana Čuček-Pleničar, Darja Eržen, Franc Srakar, Mojca Senčar, Boštjan Baebler, Jožica Červek, Alenka Vodnik-Cerar, Matej Bračko, Živa Pohar-Marinšek, Jožica Anžič, 1997, original scientific article Published in DiRROS: 16.01.2024; Views: 205; Downloads: 46
Full text (156,79 KB) |
