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322. Editorial : Mechanisms of plant host resistance against virusesRégis L. Corrêa, Marko Petek, Maite F. S. Vaslin, 2024, other scientific articles Keywords: plants, pathogens, plant viruses, resistance, biotechnological tools, CRISPR/Cas9, agriculture, biotechnology
Published in DiRROS: 05.11.2024; Views: 269; Downloads: 81
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323. How a massive open online course ForGEDI can address students' motivations, hesitancies, and obstacles in forestry-related sectorsTodora Rogelja, Ida Wallin, Barbara Öllerer, Theresa Klara Loch, Rattiya S. Lippe, Tara L. Bal, John Boakye-Danquah, Stephen Wyatt, 2024, published scientific conference contribution abstract Keywords: women in forestry, forestry, gender equality, diversity and inclusion, forestry education, future forestry workforce, transformational change, SDGs
Published in DiRROS: 05.11.2024; Views: 185; Downloads: 45
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327. Development of novel digital PCR assays for the rapid quantification of Gram-negative bacteria biomarkers using RUCS algorithmAlexandra Bogožalec Košir, Špela Alič, Viktorija Tomič, Dane Lužnik, Tanja Dreo, Mojca Milavec, 2024, original scientific article Abstract: Rapid and accurate identification of bacterial pathogens is crucial for effective treatment and infection control, particularly in hospital settings. Conventional methods like culture techniques and MALDI-TOF mass spectrometry are often time-consuming and less sensitive. This study addresses the need for faster and more precise diagnostic methods by developing novel digital PCR (dPCR) assays for the rapid quantification of biomarkers from three Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Utilizing publicly available genomes and the rapid identification of PCR primers for unique core sequences or RUCS algorithm, we designed highly specific dPCR assays. These assays were validated using synthetic DNA, bacterial genomic DNA, and DNA extracted from clinical samples. The developed dPCR methods demonstrated wide linearity, a low limit of detection (approx. 30 copies per reaction), and robust analytical performance with measurement uncertainty below 25 %. The assays showed high repeatability and intermediate precision, with no cross-reactivity observed. Comparison with MALDI-TOF mass spectrometry revealed substantial concordance, highlighting the methods’ suitability for clinical diagnostics. This study underscores the potential of dPCR for rapid and precise quantification of Gram-negative bacterial biomarkers. The developed methods offer significant improvements over existing techniques, providing faster, more accurate, and SI-traceable measurements. These advancements could enhance clinical diagnostics and infection control practices.
Keywords: digital PCR (dPCR), Gram-negative bacteria, pathogen detection, respiratory infections, biomarkers, RUCS algorithm
Published in DiRROS: 05.11.2024; Views: 160; Downloads: 345
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328. Combining ultrafast laser texturing and laser hardening to enhance surface durability by improving hardness and wear performanceAbhijit Cholkar, Suman Chatterjee, Sujith Kumar, Marko Sedlaček, Bojan Podgornik, David Kinahan, Dermot Brabazon, 2024, original scientific article Abstract: Aluminum alloy 7075 is utilized widely across marine, aerospace, and automotive sectors. However, its surface wear resistance has hindered its application in certain tribological environments. Addressing this challenge, the current study examines a hybrid laser method to increase surface wear resistance by combining two techniques: ultrafast laser texturing and laser-based surface hardening. Ultrafast laser processing is conducted using 3 W laser power, 100 kHz pulse repetition rate, 4 mm s-1 scanning speed, and three different scan patterns. After the texturing operation, laser-based surface hardening is then performed on these textures using a continuous wave laser. The laser heat treatment is conducted using laser powers of 400 and 500 W with three different scan speeds of 1, 2, and 3 mm s-1 . Microhardness evaluations show a notable increase in hardness, with the hardest sample exhibiting a 17.8% increase compared to the pristine sample. The lasertextured and laser heat-treated samples exhibit a significant reduction in the average coefficient of friction and wear volumes compared to samples that were laser-textured but not laser heat-treated. The investigated laser processing strategy offers a promising approach for surface modification, enhancing both mechanical properties and wear resistance of aluminum alloy 7075 surfaces.
Keywords: hardness, laser hardening, parametric modeling, surface modification, ultrafast laser texturing, wear resistance
Published in DiRROS: 05.11.2024; Views: 150; Downloads: 728
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329. Overlooked intersection of gender and racial/ethnic dimensions in Slovenian academic sexual harassment & violence policiesLucija Dežan, Mateja Sedmak, Zorana Medarić, 2024, original scientific article Abstract: This article addresses the need to adopt an intersectional perspective when discussing sexual harassment and violence in academia. The introduction of an intersectional perspective is important since sexual harassment and violence in relation to racial and ethnic minorities seldom revolve exclusively around gender. Recognising the intersectional nature of sexual assault and harassment means also recognising the coexistence of racism and nationalism, the perpetuation of ethnic/racial stereotypes, the multifaceted manifestations of discrimination, and a matrix of domination that exists in our society and educational and research institutions as well. In this article, we analysed official documents of Slovenian higher education and research institutions (public and private ones) with an aim to examine whether and how they address intersectionality and racial/ethnic minorities in their policies combating sexual harassment and violence. The results revealed a significant ignorance towards intersectionality of gender and ethnicity, but also issues arising from accessibility of these policies.
Keywords: sexual harassment and violence, intersectionality, gender, “race”, ethnicity, university, research institutions
Published in DiRROS: 30.10.2024; Views: 235; Downloads: 125
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330. Massively parallel measurement of protein–protein interactions by sequencing using MP3-seqAlexandr Baryshev, Benjamin Groves, Cirstyn Michel, David Baker, Ajasja Ljubetič, Georg Seelig, 2024, original scientific article Keywords: proteins, proteins interactions, sequencing, MP3-seq
Published in DiRROS: 30.10.2024; Views: 228; Downloads: 917
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