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Abstract: Mitigating pollinator declines in agriculturally dominated landscapes to safeguard pollination services requires the involvement of farmers and their willingness to adopt pollinator-friendly management. However, farmer knowledge, perceptions, and actions to support on-farm pollinators and their alignment with science-based knowledge and recommendations are rarely evaluated. To close this knowledge gap, we interviewed 560 farmers from 11 countries around the world, cultivating at least one of four widely grown pollinator-dependent crops (apple, avocado, kiwifruit, oilseed rape). We particularly focused on non-bee crop pollinators which, despite being important pollinators of many crops, received less research attention than bees. We found that farmers perceived bees to be more important pollinators than other flower-visiting insects. However, around 75% of the farmers acknowledged that non-bees contributed to the pollination of their crops, seeing them as additional pollinators rather than substitutes for bees. Despite farmers rating their own observations as being most important in how they perceived the contribution of different crop pollinator taxa, their perception aligned closely with results from available scientific studies across crops and countries. Farmer perceptions were also linked with their pollinator management practices, e.g. farmers who used managed bees for crop pollination services (more than half the farmers) rated these managed bees as particularly important. Interestingly, their willingness to establish wildflower strips or manage hedgerows to enhance pollinator visitation was linked to their ecological knowledge of non-bees or to government subsidies. Farmers adapted practices to enhance pollination services depending on the crop, which indicates an understanding of differences in the pollination ecology of crops. Almost half of the farmers had changed on-farm pollination management in the past 10 years and farm practices differed greatly between countries. This suggests integrated crop pollination measures are being adapted by farmers to reach best pollinator management practices. Our findings highlight the importance of studying local knowledge as a key to co-design locally-adapted measures to facilitate pollinator-integrated food production as ecological intensification tools.
Keywords: conservation, crop pollination, ecological intensification, farmer knowledge, local knowledge, survey
Published in DiRROS: 19.07.2024; Views: 363; Downloads: 235
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Abstract: Our experiences shape our knowledge and understanding of the world around us. The natural vibrational environment (vibroscape) is hidden to human senses but is nevertheless perceived and exploited by the majority of animals. Here, we show that the vibroscape recorded on plants in a temperate hay meadow is a dynamic low-frequency world, rich in species-specific vibrational signals. The overall vibroscape composition changed throughout the season and also depended on the plant species, as well as on the spatial position of individual plants within the meadow. Within the studied community, vibrationally signaling species sharing this communication channel avoided interference primarily by partitioning vibrational space on a fine temporal scale. The vibroscape is a reliable source of information in the environment and expands our understanding of ecological and evolutionary processes.
Published in DiRROS: 19.07.2024; Views: 386; Downloads: 260
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Abstract: Background Developing sustainable autotrophic cell factories depends heavily on the availability of robust and well-characterized biological parts. For cyanobacteria, these still lag behind the more advanced E. coli toolkit. In the course of previous protein expression experiments with cyanobacteria, we encountered inconveniences in working with currently available RSF1010-based shuttle plasmids, particularly due to their low biosafety and low yields of recombinant proteins. We also recognized some drawbacks of the commonly used fluorescent reporters, as quantification can be affected by the intrinsic fluorescence of cyanobacteria. To overcome these drawbacks, we envisioned a new chimeric vector and an alternative reporter that could be used in cyanobacterial synthetic biology and tested them in the model cyanobacterium Synechocystis sp. PCC 6803. Methods We designed the pMJc01 shuttle plasmid based on the broad host range RSFmob-I replicon. Standard cloning techniques were used for vector construction following the RFC10 synthetic biology standard. The behavior of pMJC01 was tested with selected regulatory elements in E. coli and Synechocystis sp. PCC 6803 for the biosynthesis of the established GFP reporter and of a new reporter protein, cystatin. Cystatin activity was assayed using papain as a cognate target. Results With the new vector we observed a significantly higher GFP expression in E. coli and Synechocystis sp. PCC 6803 compared to the commonly used RSF1010-based pPMQAK1. Cystatin, a cysteine protease inhibitor, was successfully expressed with the new vector in both E. coli and Synechocystis sp. PCC 6803. Its expression levels allowed quantification comparable to the standardly used fluorescent reporter GFPmut3b. An important advantage of the new vector is its improved biosafety due to the absence of plasmid regions encoding conjugative transfer components. The broadhost range vector pMJc01 could find application in synthetic biology and biotechnology of cyanobacteria due to its relatively small size, stability and ease of use. In addition, cystatin could be a useful reporter in all cell systems that do not contain papain-type proteases and inhibitors, such as cyanobacteria, and provides an alternative to fluorescent reporters or complements them.
Keywords: bacteria, cyanobacteria, synthetic biology, proteolytic cleavage, cystatin
Published in DiRROS: 19.07.2024; Views: 354; Downloads: 413
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Abstract: Increasing research demonstrates the potential of donor-derived cell-free DNA (dd-cfDNA) as a biomarker for monitoring the health of various solid organ transplants. Several methods have been proposed for cfDNA analysis, including real-time PCR, digital PCR, and next generation sequencing-based approaches. We sought to revise the droplet digital PCR (ddPCR)-based approach to quantify relative dd-cfDNA in plasma from kidney transplant (KTx) patients using a novel pilot set of assays targeting single nucleotide polymorphisms that have a very high potential to distinguish cfDNA from two individuals. The assays are capable of accurate quantification of down to 0.1% minor allele content when analyzing 165 ng of human DNA. We found no significant differences in the yield of extracted cfDNA using the three different commercial kits tested. More cfDNA was extracted from the plasma of KTx patients than from healthy volunteers, especially early after transplantation. The median level of donor-derived minor alleles in KTx samples was 0.35%. We found that ddPCR using the evaluated assays within specific range is suitable for analysis of KTx patientsʼ plasma but recommend prior genotyping of donor DNA and performing reliable preamplification of cfDNA.
Keywords: kidney transplantation, droplet digital PCR, plasma cell-free DNA, minor allele quantification, assay evaluation, graft health monitoring
Published in DiRROS: 19.07.2024; Views: 466; Downloads: 199
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Abstract: To address the decline in biodiversity, international cooperation in monitoring of threatened species is needed. Citizen science can play a crucial role in achieving this challenging goal, but most citizen science projects have been established at national or regional scales. Here we report on the establishment and initial findings of the European Stag Beetle Monitoring Network (ESBMN), an international network of stag beetle (Lucanus cervus) monitoring schemes using the same protocol. The network, started in 2016, currently includes 14 countries (see results) but with a strong variation in output regarding the number of transects (148 successful transects in total) and transect walks (1735). We found differences across European regions in the number of stag beetles recorded, related to phenology and temperature, but not for time of transect start. Furthermore, the initial experiences of the ESBMN regarding international cooperation, citizen science approach, and drop-out of volunteers is discussed. An international standardised protocol that allows some local variation is essential for international collaboration and data management, and analysis is best performed at the international level, whereas recruiting, training, and maintaining volunteers is best organised locally. In conclusion, we appeal for more joint international citizen science-based monitoring initiatives assisting international red-listing and conservation actions.
Published in DiRROS: 19.07.2024; Views: 441; Downloads: 253
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