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Abstract: This paper presents results of in-plane shear tests carried out at the ZAG laboratory in Ljubljana (Slovenia) on a RC frame with masonry infill made of clay blocks (KEBE OrthoBlock). The frame was loaded with constant vertical loads at the top of the columns and then by gradually increasing horizontal cyclic loads at the top beam level. Acquired forces and measured displacements allowed capturing hysteretic behavior for determination of dissipation energy. In addition, two Digital Image Correlation (DIC) systems, Aramis and the CivEng Vision, were used to visualize the behavior of the tested specimens, with an emphasis on computing locally required information about the behavior of highly deformable interfaces. Three types of specimens were tested in-plane: the reference specimen in form of plain RC frame, the reference specimen with constructed masonry infill without any strengthening and the specimen, previously damaged and then strengthened on both sides using glass mesh bonded to the infill and the RC frame using flexible adhesive made of polyurethane matrix (Glass Fiber Reinforced PolyUrethane - GFRPU system). The strengthening process, allowed the specimen to withstand additional cyclic loads, reaching a maximum drift of 3.6 % without serious damage disqualifying the structure from further exploitation. The GFRPU strengthening system was found to be highly effective in preventing infill collapse of damaged masonry infill wall during in-plane loading. Additionally, the results of extended thermal analysis of PU are presented as polymers are, in general, a material, poorly resistant to heat. However, the analyzed PU manifested stable properties up to 200 degrees Celsius, which makes this material promising in civil engineering applications at elevated temperatures.
Keywords: masonry blocks, damaged infill, fiber Reinforced PolyUrethane, external composite strengthening, in-plane shear, thermal tests, DIC measurements
Published in DiRROS: 14.08.2024; Views: 46; Downloads: 60
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Abstract: Paper presents selected results of the diagnostic load testing of a newly-built footbridge in Novo Mesto, Slovenia. The bridge is significant as the first bridge in Slovenia being built with the stress ribbon technology. The diagnostic load testing took place in March 2023 and comprised of three-stage static load testing, dynamic testing with a light truck driving over artificial obstacles, and measurement of ambient vibrations. The consistency between the actual response of the bridge and the response of the finite element (FE) model, used in the design, was evaluated by comparing vertical displacements from the static load testing, natural frequencies and mode shapes. The comparison of the results indicates a satisfactory agreement between the measurements and the response of the FE model, confirming the appropriateness of the employed FE model.
Keywords: bridge, bridge response measurements, stress ribon, load test
Published in DiRROS: 14.08.2024; Views: 16; Downloads: 56
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Abstract: Background. Various electrofusion parameters have to be adjusted to obtain theoptimal electrofusion efficiency. Based on published data, good electrofusion conditions can be achieved with the hypotonic treatment. However, the duration of the hypotonic treatment before electroporation and buffer hypoosmolarity have to be adjusted in order to cause cell swelling, to avoid regulatory volume decrease and to preserve cell viability. The aims of our study were to determine cell size dynamics and viability of four different cell lines in hypotonic buffer and to study the influence of the electroporation on the selected cell line in hypotonic buffer. Materials and methods. Cell size dynamics of different cell lines exposed to hypotonic buffer and electroporation were analyzed by time-resolvedcell size measurements. The viability of hypotonically treated oržand electroporated cells was determined 24 h after the experiment by a modified crystal violet (CV) viability assay. Results. In our experimental conditions the hypotonic treatment at 100 mOsm was efficient for CHO, V79 and B16-F1 cell lines. The optimal duration of the treatment was between two and five minutes. On the other hand the same hypotonic treatment did not cause cell swelling of NS1 cells. Cell swelling was also observed after electroporation of B16-F1 in isotonic buffer and it was amplified when hypotonic buffer was used. In addition, the regulatory volume decrease was successfully inhibited with electroporation. Conclusions. Cell size dynamicsin hypotonic conditions should be studied for each cell line since they differ in their sensitivity to the hypotonic treatment. The inhibition of cell regulatory volume decrease by electroporation may be beneficial in achieving higher electrofusion efficiency. (Abstract truncated at 2000 characters)
Keywords: hypotonic treatment, cell swelling, regulatory volume decrease, cell size measurements, viability, electrofusion, electroporation
Published in DiRROS: 08.03.2024; Views: 338; Downloads: 102
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Keywords: Al-Ti-B grain-refiners, quality, TiB2 particles, Al3Ti particles, electrical resistance measurements
Published in DiRROS: 01.02.2024; Views: 363; Downloads: 177
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Abstract: When creating a simulation model to assess the performance of buildings, there is usually a lack of feedback information. Only in the case of measurements of a real building is a direct comparison of the measured values and simulated results possible. Parameter data related to users’ behavior or other events can also be obtained. Their evaluated frequency, magnitude and duration, along with boundary conditions, are crucial for the results. It is clear that none of them can be predicted very accurately. Most of them, however, are needed for computer modeling. In this paper we analyzed the well-defined TRNSYS simulation model of offices equipped with radiant ceiling panels for heating and cooling. The model was based on real case offices and was validated based on measurements for 1 year. The analysis included simulations in order to define what effect the parameters related mainly to users have on the energy use and the indoor air temperatures. The study confirmed that specific human activities influence the annual energy use to a relatively small degree and that their effects often counteract. It also confirmed the even more important fact that although small, these activities can influence the thermal comfort of users. It is believed that despite the fact that this research was based on an analysis of offices equipped with radiant ceiling panels, most of the results could be applied generally.
Keywords: measurements, modeling, simulation, validation, analysis, energy use, temperature
Published in DiRROS: 15.09.2023; Views: 515; Downloads: 247
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