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952. Extinction risk alert: evaluating conservation trends for the Golden-dimpled Ground Beetle (Carabus clatratus auraniensis J. Müller, 1903) in Western BalkansAndreja Brigić, Lea Ružanović, Mihaela Kristina Mlinarić, Snježana Vujčić-Karlo, Toni Koren, Al Vrezec, Andrej Kapla, 2025, original scientific article Abstract: Due to extensive habitat loss and specialised ecological niche, the Golden-dimpled Ground Beetle (Carabus clatratus) became a locally extinct species with fragmented distribution throughout Europe. The subspecies C. clatratus auraniensis J. Müller, 1903 was described from the Vrana Lake in the Mediterranean biogeographical region of Croatia. This study aims to: assess conservation status of C. clatratus auraniensis in the Western Balkans through a chronogeonemy analysis; evaluate habitat suitability at the type locality by analyzing historical land use changes; and determine the effectiveness of protected area networks in ensuring the long-term survival of the species in core area of its distribution in Western Balkans. The chronogeonemy analysis reveals the extinction of C. clatratus auraniensis in the Mediterranean biogeographical region, including in its type locality. This is most likely caused by land use changes such as ongoing habitat degradation and wetland drainage, primarily driven by urbanisation and agriculture. However, the gap analysis indicated a good coverage by protected areas, contradicting previous results. The protected areas were established after the subspecies extinction and therefore have no impact on its conservation success. This indicates that gap analysis must be combined with other methods, e.g. chronogeonemy analysis, in order to detect real-time conservation trends. The conservation status of C. clatratus auraniensis in the Continental biogeographical region is stable, with continuous occurrence since the late 19th century and an increase in records in the last three decades, even with long dispersal records (e.g. Mura River in NE Slovenia). Records increase are linked to large well-preserved semi-natural wetlands along the Sava, Drava and Danube rivers, reflecting the subspecies’ good prospects. Reintroduction into protected areas in the Mediterranean region of Croatia could be a viable strategy to support the survival of this subspecies, enhancing the conservation prospects for rare C. clatratus in Europe.
Keywords: chronogeonemy analysis, Croatia, gap analysis, habitat loss, land use change, local extinction, protected areas
Published in DiRROS: 25.09.2025; Views: 231; Downloads: 119
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953. Citizen science for monitoring plastic pollution from source to sea: a systematic review of methodologies, best practices, and challengesCorinne Corbau, Alexandre Lazarou, Oliver Bajt, Vlatka Filipović Marijić, Tatjana Simčič, Massimo Coltorti, Elisa Pignoni, Umberto Simeoni, 2025, review article Abstract: Citizen science provides a valuable approach for tracking plastic pollution; however, its effectiveness is often limited by methodological inconsistencies, concerns about data quality, and a persistent gap between data collection and policy implementation. This systematic review addresses the key question: What constitutes a comprehensive set of best practices for addressing these issues and enhancing the scientific and societal impact of citizen science in monitoring plastic pollution from source to sea? Analyzing 84 studies, from beach cleanups to microplastic sampling, this review synthesizes best practices and identifies remaining gaps. It presents a structured framework designed to enhance data quality and volunteer participation. Key challenges include the ‘microplastic analytical bottleneck,’ the ‘digital divide,’ and notable geographical and demographic disparities that hinder the integration of policies. While citizen science is effective for large-scale data collection, its main challenge is translating data into actionable policies. The main contribution of this review is a series of practical recommendations aimed at improving methodological consistency, ensuring fair volunteer participation, and facilitating the transition from citizen data to evidence-based environmental management, thereby enhancing the effectiveness and impact of citizen science.
Keywords: public engagement, plastic pollution data quality, environmental monitoring, policy integration
Published in DiRROS: 25.09.2025; Views: 259; Downloads: 137
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955. Agents of European beech declineNikica Ogris, Ana Brglez, Andreja Kavčič, Janja Zajc Žunič, Maarten De Groot, Barbara Piškur, 2025, complete scientific database of research data Abstract: Data collected in the project "Diseases, pests and drought stress of European beech at various climate change scenarios (V4-2026), Working package 2: Causes of the decline of European beech in Slovenia". Data was collected on 40 sampling plots, 39 plots on systematic grid 16 × 16 km and one additional plot in the Alps. On each sampling plot two trees were felled, altogether 80 trees: one visually healthy, and one damaged tree. Visual assessment of crown condition and damaging agents was evaluated following methods of ICP Forests. Rhizosphere soil was collected from each sampled tree for the Phytophthora baiting assay. For fungal isolations five parts of a tree were sampled: leaves, twigs (up to 20 mm in diameter), branches (over 20 mm in diameter), trunk, surface roots and root collar (up to 25 cm from the ground). Four samples from each tree part were collected. Finally, 20 samples per tree and 40 samples per plot were collected, yielding 1.600 samples in total. The samples were surface sterilized. Four subsamples were collected from one sample, altogether, 6.400 subsamples/tissue pieces. Four subsamples were plated on potato dextrose agar supplemented with streptomycin. Fungi and Phytophthora species were identified using molecular techniques, while insects were identified based on their morphological characteristics.
Keywords: research data, Fagus sylvatica, decline, damage, complex disease, fungi, insects, Phytophthora, endophytes, stress
Published in DiRROS: 25.09.2025; Views: 329; Downloads: 2
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958. Data from : Physical constraints and environmental factors shape phloem anatomical traits in woody angiosperm speciesYan Wang, Johannes Liesche, Alan Crivellaro, Jiří Doležal, Jan Altman, Donato Chiatante, Anastazija Dimitrova, Ze-Xin Fan, Peili Fu, Félix Forest, Jožica Gričar, 2025, complete scientific database of research data Abstract: We collected main stem or branch cross-sections of 188 angiosperm woody species, which represent a wide range of climates and diverse families. Measurements of xylem vessel and phloem sieve element diameter, density, and lumen fraction were used in phylogenetic structural equation models to disentangle internal and climatic constraints on their morphological and anatomical features.
Keywords: adaptation, allometry, phloemsieve element, xylem vessel
Published in DiRROS: 25.09.2025; Views: 304; Downloads: 134
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959. Physical constraints and environmental factors shape phloem anatomical traits in woody angiosperm speciesYan Wang, Johannes Liesche, Alan Crivellaro, Jiří Doležal, Jan Altman, Donato Chiatante, Anastazija Dimitrova, Ze-Xin Fan, Peili Fu, Félix Forest, Jožica Gričar, 2025, original scientific article Abstract: Xylem trait studies have enhanced our understanding of how plants strategically adapt their morphological and anatomical features to diverse climates. Despite the importance of the phloem in plant functioning, similar studies of phloem traits are lacking. To tackle this knowledge gap, we analyzed phloem anatomical traits of woody angiosperm species in relation to climate and the distance of samples to the stem tip. We collected main stem or branch cross-sections of 188 angiosperm woody species, which represent a wide range of climates and diverse families. Measurements of xylem vessel and phloem sieve element diameter, density, and lumen fraction were used in phylogenetic structural equation models to disentangle internal and climatic constraints on their morphological and anatomical features. Our results showed that distance-to-tip mainly affects sieve element and vessel diameter and density, while climate more strongly influenced conduit lumen fraction. Vessel size was positively correlated with temperature after correcting for the distance-to-tip, while sieve element diameter was correlated with water availability. Our results highlight the need to account for distance-to-tip when accessing anatomical variations linked to the environment, and show that sieve element traits respond to other climatic drivers than vessel traits rather than simply mirroring them.
Keywords: adaptation, allometry, phloemsieve element, tip-to-base conduit widening, xylem vessel
Published in DiRROS: 25.09.2025; Views: 272; Downloads: 147
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960. Digital PCR-based genotyping: a precision approach to HCMV drug resistanceMojca Milavec, Tašja Cvelbar, Alexandra Bogožalec Košir, 2025, independent scientific component part or a chapter in a monograph Abstract: The genotyping workflow described uses digital PCR (dPCR) to detect and quantify drug resistance mutations in human cytomegalovirus (HCMV). The method focuses on the detection and quantification of three common mutations in the UL97 gene at codons 460, 594, and 595, which are responsible for the majority of ganciclovir-resistant clinical isolates. The dPCR approach offers high sensitivity and accuracy, making it suitable for routine testing as well as a reference measurement procedure for external quality assessment schemes. The workflow includes several key steps: DNA isolation, preparation of the dPCR reaction mixture, partitioning, thermocycling, and data analysis. This method improves the detection capabilities of HCMV drug resistance and provides a robust and efficient tool for clinical and research applications.
Keywords: digital PCR, human cytomegalovirus, antimicrobial drug resistance, mutations
Published in DiRROS: 25.09.2025; Views: 239; Downloads: 41
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