1. Validation of molecular tests for the detection of tomato brown rugose fruit virus (ToBRFV) in seed of tomato and pepperAnne Giesbers, Annelien J. W. Roenhorst, Martijn Schenk, Ruud Barnhoorn, Laura Tomassoli, Marta Luigi, Kris De Jonghe, Laëtitia Porcher, Pascal Gentit, Heiko Ziebell, Mouhammad Zeidan, Dorit Shargil, Sabine Grausgruber Groeger, Nataša Mehle, 2021, final research report Abstract: Tomato brown rugose fruit virus (ToBRFV) is a relatively recently described tobamovirus (Salem et al., 2016) causing problems in tomato and pepper cultivation worldwide (Luria et al., 2017, Salem et al., 2019). ToBRFV has been reported to cause yellow spots, green spots, necrotic lesions and occasional rugose symptoms on tomato fruits, chlorosis and mosaic symptoms on leaves and occasional leaf narrowing (Cambrón-Crisantos et al., 2018; Luria et al., 2017; Menzel et al., 2019). For pepper, stunting of young plants, puckering and yellow mottling of leaves, and misshapen fruits have been reported (Salem et al., 2019). These symptoms render affected fruits non-marketable.
Published in DiRROS: 04.09.2024; Views: 101; Downloads: 68
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2. TPS reports with description of the method, materials and software used, as well as the data analysis - Round 2, Version 1.0 : grant agreement N. 773139Géraldine Anthoine, Ian Brittain, Aude Chabirand, Tanja Dreo, Francesco Faggioli, Catherine Harrison, Marta Luigi, Tadeja Lukežič, Slavica Matić, Nataša Mehle, Monica Mezzalama, Hanna Mouaziz, Maja Ravnikar, Jean-Philippe Renvoise, Davide Spadaro, Laura Tomassoli, Ana Vučurović, 2021, treatise, preliminary study, study Abstract: The aim of the deliverable 1.4. is to present a summary of the results obtained in the Round 1 of the test performance studies (TPS) organized by WP1 on six prioritized pests. Tests selection for each TPS was conducted following the “Common rules for selection of tests for TPS” and based on the “Weighted criteria for selection of tests for TPS”, both described in deliverable D1.1, while the list of selected tests for each TPS is available and explained in deliverable D1.2. TPS participants were selected following the “Common rules for selection of participants for TPS” and based on the “Criteria for selection of participants of TPS”, also both described in deliverable D1.1. For each of the six TPSs, the methodology used to perform the tests, the results of preliminary studies to select the tests, the results of the TPS and their thorough analysis and interpretation are described in corresponding TPS reports (supplementary information available upon request under confidentiality agreement). The validation data obtained during the six TPSs will be available in the validation section of the EPPO database on the diagnostic expertise. Main outcomes for each of the TPSs are highlighted as well as difficulties noticed during the organization process, which will improve organization of the following studies in the Round 2.
Keywords: test performance study, plant diseases
Published in DiRROS: 03.09.2024; Views: 143; Downloads: 66
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3. Testing of tomato brown rugose fruit virus by real-time RT-PCR (Bernabe-Orts et al. 2021) : validation reportNataša Mehle, Marta Luigi, Antonio Tiberini, Ariana Manglli, Ana Vučurović, Francesco Faggioli, 2023, final research report Abstract: Detection and identification of tomato brown rugose fruit virus.
Keywords: ToBRFV, diagnostics, method validation, EURL-Virology
Published in DiRROS: 02.09.2024; Views: 119; Downloads: 89
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4. What did we achieve with VALITEST an EU project on validation in plant pest diagnostics?Charlotte Trontin, Barbara Agstner, Denise Altenbach, Géraldine Anthoine, Hanna Baginska, Ian Brittain, A Chabirand, Anne-Marie Chappé, P. Dahlin, Tanja Dreo, C. Freye-Minks, Camilo Gianinazzi, Catherine Harrison, Glyn Jones, Marco Stefan Kaiser, Marta Luigi, Sébastien Massart, Nataša Mehle, Monica Mezzalama, Hanna Mouaziz, Maja Ravnikar, Tom Raaymakers, Jean-Philippe Renvoise, Mathieu Rolland, Marta Santos, Sam Seddas, René van der Vlugt, Ana Vučurović, Françoise Petter, 2022, original scientific article Keywords: plant pest diagnostics, validation, test performance study, high-throughput, sequencing, reference material, training
Published in DiRROS: 17.07.2024; Views: 221; Downloads: 98
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5. Development and validation of a one-step reverse transcription real-time PCR assay for simultaneous detection and identification of tomato mottle mosaic virus and tomato brown rugose fruit virusAntonio Tiberini, Ariana Manglli, Anna Taglienti, Ana Vučurović, Jakob Brodarič, Luca Ferretti, Marta Luigi, Andrea Gentili, Nataša Mehle, 2022, original scientific article Abstract: Tobamovirus species represent a threat to solanaceous crops worldwide, due to their extreme stability and because they are seed borne. In particular, recent outbreaks of tomato brown rugose fruit virus in tomato and pepper crops led to the establishment of prompt control measures, and the need for reliable diagnosis was urged. Another member of the genus, tomato mottle mosaic virus, has recently gained attention due to reports in different continents and its common features with tomato brown rugose fruit virus. In this study, a new real-time RT-PCR detection system was developed for tomato brown rugose fruit virus and tomato mottle mosaic virus on tomato leaves and seeds using TaqMan chemistry. This test was designed to detect tomato mottle mosaic virus by amplifying the movement protein gene in a duplex assay with the tomato brown rugose fruit virus target on the CP-3’NTR region, which was previously validated as a single assay. The performance of this test was evaluated, displaying analytical sensitivity 10−5–10−6-fold dilution for seeds and leaves, respectively, and good analytical specificity, repeatability, and reproducibility. Using the newly developed and validated test, tomato brown rugose fruit virus detection was 100% concordant with previously performed analyses on 106 official samples collected in 2021 from different continents.
Keywords: real-time PCR, tomato mottle mosaic virus, tomato brown rugose fruit virus, leaves detection, seeds detections, performance criteria
Published in DiRROS: 16.07.2024; Views: 207; Downloads: 183
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