861. Bimodal dynamics of primary metabolism-related responses in tolerant potato-Potato virus Y interactionNeža Turnšek, Živa Ramšak, Katja Stare, Tjaša Stare, Dominik Vodnik, Andrej Blejec, Kristina Gruden, Wolfram Weckwerth, Stefanie Wienkoop, 2015, original scientific article Abstract: Background
Potato virus Y (PVY) is a major pathogen that causes substantial economic losses in worldwide potato production. Different potato cultivars differ in resistance to PVY, from severe susceptibility, through tolerance, to complete resistance. The aim of this study was to better define the mechanisms underlying tolerant responses of potato to infection by the particularly aggressive PVYNTN strain. We focused on the dynamics of the primary metabolism-related processes during PVYNTN infection.
Results
A comprehensive analysis of the dynamic changes in primary metabolism was performed, which included whole transcriptome analysis, nontargeted proteomics, and photosynthetic activity measurements in potato cv. Désirée and its transgenic counterpart depleted for accumulation of salicylic acid (NahG-Désirée). Faster multiplication of virus occurred in the NahG-Désirée, with these plants developing strong disease symptoms. We show that while the dynamics of responses at the transcriptional level are extensive and bimodal, this is only partially translated to the protein level, and to the final functional outcome. Photosynthesis-related genes are transiently induced before viral multiplication is detected and it is down-regulated later on. This is reflected as a deficiency of the photosynthetic apparatus at the onset of viral multiplication only. Interestingly, specific and constant up-regulation of some RuBisCO transcripts was detected in Désirée plants, which might be important, as these proteins have been shown to interact with viral proteins.
In SA-deficient and more sensitive NahG-Désirée plants, consistent down-regulation of photosynthesis-related genes was detected. A constant reduction in the photochemical efficiency from the onset of viral multiplication was identified; in nontransgenic plants this decrease was only transient. The transient reduction in net photosynthetic rate occurred in both genotypes with the same timing, and coincided with changes in stomatal conductivity.
Conclusions
Down-regulation of photosynthesis-related gene expression and decreased photosynthetic activity is in line with other studies that have reported the effects of biotic stress on photosynthesis. Here, we additionally detected induction of light-reaction components in the early stages of PVYNTN infection of tolerant interaction. As some of these components have already been shown to interact with viral proteins, their overproduction might contribute to the absence of symptoms in cv. Désirée.
Keywords: plant-pathogen interactions, Potato virus Y, potyviridae, salicylic acid, whole transcriptome analysis, shot-gun proteomics, photosynthetic parameters
Published in DiRROS: 29.07.2024; Views: 305; Downloads: 280
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862. oVERFLOw project : Vulnerability assessment of embankments and bridges exposed to flooding hazards : Deliverable 6.4Valentina Bau', Mirko Kosič, Andrej Anžlin, Mario Bacic, 2022, final research report Abstract: This deliverable is a report on the development of a strategy for adaptation measures to flood events for the embankments, riverbanks and bridges, which can afterwards be used for the case study areas considered in the Overflow project. The deliverable presents a comprehensive approach that enables the choice of the best adaptation measures to overcome the vulnerability of critical infrastructure to flooding events. The most suitable countermeasures can therefore be selected based on the outcomes of the vulnerability assessment and risk forecasting tool. Such a strategy will be essential for the relevant authorities and IMs. The strategy proposes optimal adaptation measures depending on areas specificities, incorporating socio-economic and environmental multi-objective optimization.
Keywords: adaptation measures, countermeasures, vulnerability, flooding, resilience, embankments, bridges
Published in DiRROS: 29.07.2024; Views: 273; Downloads: 160
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863. Characterization of bloom iron smelting site remains in Pržanj, SloveniaJaka Burja, Barbara Šetina, Daša Pavlovič, 2024, original scientific article Abstract: This paper gives an overview of findings, connected with metallurgical activity, at the Pržanj archeological site near Ljubljana, Slovenia. More than 230 kg of slag and other remains connected with early medieval (from the 5th to the 12th century AD) metallurgical activities was found at the excavation site. The remains were grouped into four categories, i.e., furnace remains, ore, slag and a ferrous product, and analyzed in detail to obtain their chemical composition, microstructural characteristics, and mineral phase composition. The furnace wall remains, identified by their morphology and chemical composition, revealed an intensive iron processing activity at the site. The iron ore at the site was identified as goethite (FeO(OH)), a surprising find in Slovenia where limonite is typically used, and its presence suggests the potential exploitation of local bog iron ore, given the site’s geological context. Abundant slag remains at the site, identified by their shape, molten microstructure, and mineral components like wuestite, fayalite, and hercynite, indicated sophisticated smelting practices, including the use of CaO-rich materials to lower the melting temperature, a technique likely preserved from antiquity. Findings of ferrous products at ancient metallurgical sites are rare due to their value, but the discovery of a corroded iron bloom conglomerate at this site, initially mistaken for furnace remains, highlights the challenges in identifying small, corroded ferrous fragments that are often misidentified as ore. The results indicate extensive metallurgical activity at the excavation site, marking it as the first documented early medieval iron smelting production site in Slovenia.
Keywords: bloom iron, bloomery slag, archaeometallurgy, microstructure
Published in DiRROS: 29.07.2024; Views: 301; Downloads: 225
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864. Kakšen vpliv bo imela Evropska unija na izvedbo del v gozdovih?Amina Gačo, Matjaž Dovečar, Darja Stare, 2024, professional article Keywords: gozdarstvo, pridobivanje lesa, gozdarska dela, evropska zakonodaja, zakonodaja, Evropa
Published in DiRROS: 27.07.2024; Views: 320; Downloads: 160
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865. Pregled okoljskih, ekonomskih in socialnih meril za obravnavane tehnološke postopke pridobivanja lesaMartin Jež, Janez Krč, Matevž Triplat, 2024, professional article Keywords: gozdarstvo, pridobivanje lesa, okoljska merila, ekonomska merila, socialna merila
Published in DiRROS: 27.07.2024; Views: 338; Downloads: 173
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