1. Fast and accurate multiplex identification and quantification of seven genetically modified soybean lines using six-color digital PCRAlexandra Bogožalec Košir, Sabine Muller, Jana Žel, Mojca Milavec, Allison C. Mallory, David Dobnik, 2023, original scientific article Abstract: The proliferation of genetically modified organisms (GMOs) presents challenges to GMO testing laboratories and policymakers. Traditional methods, like quantitative real-time PCR (qPCR), face limitations in quantifying the increasing number of GMOs in a single sample. Digital PCR (dPCR), specifically multiplexing, offers a solution by enabling simultaneous quantification of multiple GMO targets. This study explores the use of the Naica six-color Crystal dPCR platform for quantifying five GM soybean lines within a single six-plex assay. Two four-color assays were also developed for added flexibility. These assays demonstrated high specificity, sensitivity (limit of detection or LOD < 25 copies per reaction) and precision (bias to an estimated copy number concentration <15%). Additionally, two approaches for the optimization of data analysis were implemented. By applying a limit-of-blank (LOB) correction, the limit of quantification (LOQ) and LOD could be more precisely determined. Pooling of reactions additionally lowered the LOD, with a two- to eight-fold increase in sensitivity. Real-life samples from routine testing were used to confirm the assays’ applicability for quantifying GM soybean lines in complex samples. This study showcases the potential of the six-color Crystal dPCR platform to revolutionize GMO testing, facilitating comprehensive analysis of GMOs in complex samples. Keywords: digital PCR, dPCR, quantification, multiplexing, genetically modified organisms, 6-color system, virus diagnostics, virology Published in DiRROS: 29.03.2024; Views: 163; Downloads: 64 Link to file This document has many files! More... |
2. Percutaneous transluminal renal angioplasty - a multicentre study of the long term results : Belan A.... [et al.]A. Belan, Dušan Pavčnik, A. Petrosijan, L. Horvath, I. Rabkin, A. H. Muller, Miloš Šurlan, Janez Klančar, Jernej Knific, Pavel Berden, D. Vidmar, Rok Cesar, Igor Kocijančič, Staša Kaplan-Pavlovčič, 1990, original scientific article Published in DiRROS: 18.09.2023; Views: 307; Downloads: 97 Full text (524,36 KB) |
3. Gospodarjenje s tujerodnimi drevesnimi vrstami v urbanem prostoru alpskega prostoraAjša Alagić, Aleksander Marinšek, Ana Dolenc, Andrey Krasovskiy, Anica Simčič, Anja M. Bindewald, Anja Müller-Meißner, Bénédicte Baxerres, Claudia Cocozza, Debojyoti Chakraborty, Janez Kermavnar, Lado Kutnar, Simon Zidar, Tina Trampuš, Živa Bobič Červek, 2022, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: tujerodne drevesne vrste, Alpe, invazivne drevesne vrste, gospodarjenje z gozdovi, podnebne spremembe, urbani prostor Published in DiRROS: 12.08.2022; Views: 1188; Downloads: 266 Full text (4,27 MB) |
4. Handbook for non native tree species in the urban spaceAjša Alagić, Aleksander Marinšek, Ana Dolenc, Andrey Krasovskiy, Anica Simčič, Anja M. Bindewald, Anja Müller-Meißner, Bénédicte Baxerres, Claudia Cocozza, Debojyoti Chakraborty, Janez Kermavnar, Lado Kutnar, Simon Zidar, Tina Trampuš, Živa Bobič Červek, 2022, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: non-native tree species, Alps, invasive alien species, forest management, urbanspace, climate changes Published in DiRROS: 12.08.2022; Views: 573; Downloads: 207 Full text (4,25 MB) |
5. Priročnik za prepoznavanje tujerodnih drevesnih vrst v alpskem prostoruAjša Alagić, Bénédicte Baxerres, Frédéric Berger, Anja M. Bindewald, Sylvain Bouquet, Giuseppe Brundu, Debojyoti Chakraborty, Patricia Detry, Freya-Isabel Georges, Quentin Guillory, Janez Kermavnar, Lado Kutnar, Katharina Lapin, Aleksander Marinšek, Eric Mermin, Anja Müller-Meißner, Janine Oettel, Anica Simčič, Simon Zidar, 2022, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: tujerodne drevesne vrste, Alpe, invazivne drevesne vrste Published in DiRROS: 12.08.2022; Views: 1271; Downloads: 401 Full text (19,49 MB) |
6. Identification handbook for non native tree species in the Alpine spaceAjša Alagić, Bénédicte Baxerres, Frédéric Berger, Anja M. Bindewald, Sylvain Bouquet, Giuseppe Brundu, Debojyoti Chakraborty, Patricia Detry, Freya-Isabel Georges, Quentin Guillory, Janez Kermavnar, Lado Kutnar, Katharina Lapin, Aleksander Marinšek, Eric Mermin, Anja Müller-Meißner, Janine Oettel, Anica Simčič, Simon Zidar, 2022, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: non-native tree species, Alps, invasive alien species Published in DiRROS: 12.08.2022; Views: 622; Downloads: 216 Full text (17,57 MB) |