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Title:Določanje klonalnosti limfoidnih proliferacij : uvedba nove metode in primerjava z dosedanjo metodo
Authors:ID Škerl, Petra (Author)
ID Koković, Ira (Author)
ID Kloboves-Prevodnik, Veronika (Author)
ID Novaković, Srdjan (Author)
Files:.pdf PDF - Presentation file, download (1,37 MB)
MD5: 9021BC258658695D5A6AFF52FA5966CB
PID: 20.500.12556/dirros/dce5385a-eb3c-4cec-a9c0-77d070254be9
 
Language:Slovenian
Typology:1.04 - Professional Article
Organization:Logo OI - Institute of Oncology
Abstract:Na Oddelku za molekularno diagnostiko smo na področju diagnostike limfomov uvedli dodatno metodo za določanje klonalnosti limfoidnih proliferacij. Tudi nova metoda temelji na verižni reakciji s polimerazo (PCR) in na uporabi različnih konsenzusnih oligonukleotidnih začetnikov. Metoda je validirana in registrirana za diagnostične potrebe (oznaka CE). Z razširjenim naborom oligonukleotidnih začetnikov in uporabo večjega števila začetnikov smo, po naših izračunih, izboljšali občutljivost določanja klonalnosti limfocitov B za 23,1 %, določanje klonalnosti limfocitov T pa za 14,3 %. Teoretično lahko sedaj z obema metodama določimo monoklonalno populacijo limfocitov pri 84,6 % B- in 85,7 % T-limfomov
Publication status:Published
Publication version:Version of Record
Year of publishing:2009
Number of pages:str. 133-136, 153
Numbering:Letn. 13, št. 2
PID:20.500.12556/DiRROS-8748 New window
UDC:616.15-006.6-07
ISSN on article:1408-1741
URN:URN:NBN:SI:doc-CZWG1MCS
COBISS.SI-ID:847995 New window
Copyright:by Authors
Note:Soavtorji: I. Koković, V. Prevodnik Kloboves in S. Novaković;
Publication date in DiRROS:31.08.2018
Views:4148
Downloads:1025
Metadata:XML DC-XML DC-RDF
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ŠKERL, Petra, KOKOVIĆ, Ira, KLOBOVES-PREVODNIK, Veronika and NOVAKOVIĆ, Srdjan, 2009, Določanje klonalnosti limfoidnih proliferacij : uvedba nove metode in primerjava z dosedanjo metodo. Onkologija [online]. 2009. Vol. 13, no. 2, p. 133–136, 153. [Accessed 22 March 2025]. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=8748
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Record is a part of a journal

Title:Onkologija
Shortened title:Onkologija
Publisher:Onkološki inštitut
ISSN:1408-1741
COBISS.SI-ID:65324032 New window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:31.08.2018

Secondary language

Language:English
Title:Determination of Lymphoid Proliferation Clonality – A New Method in Comparison to the in House Method
Abstract:At the Department of Molecular Diagnostics, a new method for determining lymphoid proliferation clonality has been introduced in lymphoma diagnostics. The method is based on PCR and on the use of various consensus primers. It is validated and registered for the use in clinical diagnostics (CE-marked method). Our measurements confirmed that the use of extended number of primers improved the sensitivity of determining the lymphoid clonality of B-lymphocytes and T-lymphocytes by 23.1% and 14.3%, respectively. Both methods may, theoretically, be used in determining monoclonal population of lymphocytes in 84.6% of B- and 85.8 of T-lymphomas.


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