| Naslov: | Llimited efficacy of nanoparticle-assisted electroporation for membrane permeabilization and gene electrotransfer |
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| Avtorji: | ID Polajžer, Tamara (Avtor)
ID Kranjc, Matej (Avtor)
ID Kralj, Slavko, Institut "Jožef Stefan" (Avtor)
ID Caf, Maja, Institut "Jožef Stefan" (Avtor)
ID Romih, Rok (Avtor)
ID Hudoklin, Samo (Avtor)
ID Rocca, Federica (Avtor)
ID Miklavčič, Damijan (Avtor) |
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| Datoteke: |  PDF - Predstavitvena datoteka, prenos (3,47 MB)
MD5: D9A73E35F5797BB573237A033524E353
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  IJS - Institut Jožef Stefan
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| Povzetek: | Nanoparticles (NPs) were previously explored as enhancers in electroporation due to their potential to locally amplify electric fields near cell mem branes, with gold nanoparticles (AuNPs) in particular showing promise in improving membrane permeability and gene electrotransfer (GET). In this study, we systematically investigated the influence of NP properties—including size, shape, surface functionaliza tion, and material—on electroporation efficacy. Methods: A combined approach using theoretical modeling and experimental validation was employed, encompassing numerical simulations, membrane permeabilization assays, transmission electron microscopy Numerical results revealed that the presence of NPs alters local electric field distributions, but the amplification is highly localized, regardless of NPconductivity or geometry. Experimentally, only two out of six tested NP types produced a statistically significant, yet modest, increase in membrane permeability at one electric f ield intensity. Similarly, GET improvement was observed with only one NP type, with no dependence on concentration or functionalization. Conclusions: Overall, our findings demonstrate that NPs, under tested conditions, do not substantially enhance cell membrane permeability or GET efficacy. These conclusions are supported by both computational modeling and in vitro experiments |
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| Ključne besede: | nanodelci, numerični model, elektro tansfer |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 17.06.2025 |
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| Datum sprejetja članka: | 19.07.2025 |
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| Datum objave: | 25.07.2025 |
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| Založnik: | MDPI |
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| Leto izida: | 2025 |
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| Št. strani: | str. 1-19 |
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| Številčenje: | Vol. 17, Art. 964 |
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| Izvor: | Švica |
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| PID: | 20.500.12556/DiRROS-24502  |
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| UDK: | 536 |
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| ISSN pri članku: | 1999-4923 |
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| DOI: | 10.3390/pharmaceutics17080964 |
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| COBISS.SI-ID: | 244309507 |
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| Avtorske pravice: | © 2025 by the authors. |
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| Datum objave v DiRROS: | 02.12.2025 |
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| Število ogledov: | 129 |
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| Število prenosov: | 52 |
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| Metapodatki: |    |
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