| Title: | Llimited efficacy of nanoparticle-assisted electroporation for membrane permeabilization and gene electrotransfer |
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| Authors: | ID Polajžer, Tamara (Author)
ID Kranjc, Matej (Author)
ID Kralj, Slavko, Institut "Jožef Stefan" (Author)
ID Caf, Maja, Institut "Jožef Stefan" (Author)
ID Romih, Rok (Author)
ID Hudoklin, Samo (Author)
ID Rocca, Federica (Author)
ID Miklavčič, Damijan (Author) |
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| Files: |  PDF - Presentation file, download (3,47 MB)
MD5: D9A73E35F5797BB573237A033524E353
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  IJS - Jožef Stefan Institute
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| Abstract: | Nanoparticles (NPs) were previously explored as enhancers in electroporation due to their potential to locally amplify electric fields near cell mem branes, with gold nanoparticles (AuNPs) in particular showing promise in improving membrane permeability and gene electrotransfer (GET). In this study, we systematically investigated the influence of NP properties—including size, shape, surface functionaliza tion, and material—on electroporation efficacy. Methods: A combined approach using theoretical modeling and experimental validation was employed, encompassing numerical simulations, membrane permeabilization assays, transmission electron microscopy Numerical results revealed that the presence of NPs alters local electric field distributions, but the amplification is highly localized, regardless of NPconductivity or geometry. Experimentally, only two out of six tested NP types produced a statistically significant, yet modest, increase in membrane permeability at one electric f ield intensity. Similarly, GET improvement was observed with only one NP type, with no dependence on concentration or functionalization. Conclusions: Overall, our findings demonstrate that NPs, under tested conditions, do not substantially enhance cell membrane permeability or GET efficacy. These conclusions are supported by both computational modeling and in vitro experiments |
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| Keywords: | nanodelci, numerični model, elektro tansfer |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 17.06.2025 |
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| Article acceptance date: | 19.07.2025 |
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| Publication date: | 25.07.2025 |
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| Publisher: | MDPI |
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| Year of publishing: | 2025 |
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| Number of pages: | str. 1-19 |
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| Numbering: | Vol. 17, Art. 964 |
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| Source: | Švica |
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| PID: | 20.500.12556/DiRROS-24502  |
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| UDC: | 536 |
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| ISSN on article: | 1999-4923 |
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| DOI: | 10.3390/pharmaceutics17080964 |
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| COBISS.SI-ID: | 244309507 |
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| Copyright: | © 2025 by the authors. |
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| Publication date in DiRROS: | 02.12.2025 |
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| Views: | 134 |
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| Downloads: | 54 |
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| Metadata: |    |
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