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Title:Rapid loop-mediated isothermal amplification assays for grapevine yellows phytoplasmas on crude leaf-vein homogenate has the same performance as qPCR
Authors:ID Kogovšek, Polona (Author)
ID Mehle, Nataša (Author)
ID Pugelj, Anja (Author)
ID Jakomin, Tjaša (Author)
ID Schroers, Hans-Josef (Author)
ID Ravnikar, Maja (Author)
ID Dermastia, Marina (Author)
Files:.pdf PDF - Presentation file, download (335,75 KB)
MD5: 11C1F9D71E4A5550EF502263A98754A7
 
URL URL - Source URL, visit https://doi.org/10.1007/s10658-016-1070-z
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Logo KIS - Agricultural Institute of Slovenia
Abstract:A fluorescence-based real-time loop-mediated isothermal amplification (LAMP) assay for ‘Candidatus Phytoplasama solani’ (Bois noir phytoplasma; BNp) detection was developed and optimised for rapid laboratory and on-site BNp detection. This assay is highly specific, rapid and as sensitive as qPCR. It was validated according to European and Mediterranean Plant Protection Organisation recommendations. In addition, 286 grapevine leaf samples from the 2015 growing season were tested with this new real-time LAMP assay and an assay previously developed for detection of Flavescence dorée phytoplasma (FDp). These LAMP assays for detection of both BNp and FDp used without any DNA extraction step, which is a required step for qPCR analysis, were comparably effective to qPCR, and positive results were obtained in less than 35 min.
Keywords:real-time LAMP, grapevine yellows phytoplasma, validation
Publication status:Published
Publication version:Version of Record
Publication date:01.01.2017
Year of publishing:2017
Number of pages:str. 75-84
Numbering:Vol. 148, iss. 1
PID:20.500.12556/DiRROS-19701 New window
UDC:581.2
ISSN on article:0929-1873
DOI:10.1007/s10658-016-1070-z New window
COBISS.SI-ID:4088399 New window
Publication date in DiRROS:24.07.2024
Views:646
Downloads:342
Metadata:XML DC-XML DC-RDF
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KOGOVŠEK, Polona, MEHLE, Nataša, PUGELJ, Anja, JAKOMIN, Tjaša, SCHROERS, Hans-Josef, RAVNIKAR, Maja and DERMASTIA, Marina, 2017, Rapid loop-mediated isothermal amplification assays for grapevine yellows phytoplasmas on crude leaf-vein homogenate has the same performance as qPCR. European journal of plant pathology [online]. 2017. Vol. 148, no. 1, p. 75–84. [Accessed 3 April 2025]. DOI 10.1007/s10658-016-1070-z. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19701
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Record is a part of a journal

Title:European journal of plant pathology
Shortened title:Eur. j. plant pathol.
Publisher:Kluwer Academic Publishers
ISSN:0929-1873
COBISS.SI-ID:336759 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:L4-5525-2013
Name:Študij epidemiologije in raznolikosti mikrobnih povzročiteljev bolezni rastlin
Funder:EC - European Commission
Funding programme:FP7 ERA-Net Project
Name:Core Organic II

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

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